A new method to process testicular sperm: combining enzymatic digestion, accumulation of spermatozoa, and stimulation of motility

Wöber, Martina; Ebner, Thomas; Steiner, Sarah L.; Strohmer, Heinz; Oppelt, Peter; Plas, Eugen; Obruca, A.

doi:10.1007/s00404-014-3458-3

Cited by 12 publications

(9 citation statements)

References 34 publications

(45 reference statements)

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“…The above mentioned benefits of theophylline led to higher fertilization rates, developmental potential, and superior blastocyst morphology. These results are in agreement with a later study performed by Wober and colleagues, where the addition of theophylline enhanced motility in 24 out of 28 samples and led to better fertilization rates, blastocyst formation, implantation, and clinical pregnancy rates [Wober et al 2015].…”

Section: Methylxanthines: Pentoxifylline and Theophyllinesupporting

confidence: 93%

Improving ICSI: A review from the spermatozoon perspective

Simopoulou

Gkoles

Bakas

et al. 2016

Systems Biology in Reproductive Medicine

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ICSI: intracytoplasmic sperm injection; IVF: in vitro fertilization; PGD: reimplantation genetic diagnosis; IVM: in vitro maturation; HCV/HIV: hepatitis C virus/human immunodeficiency virus; IMSI: intracytoplasmic morphologically selected sperm injection; DGC: density gradient centrifugations; S-U: swim-up; ART: assisted reproduction technology; IUI: intrauterine insemination; PVP: polyvinylpyrrolidone; HA: hyaluronic acid; MSOME: motile sperm organelle morphology examination; ZP: zona pellucida; MACS: magnetic activation cell sorting; HOST: hypo-osmotic swelling test; TESE: testicular sperm extraction; MMP: mitochondrial membrane potential; OAT: oligoasthenoteratozoospermic.

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Section: Methylxanthines: Pentoxifylline and Theophyllinesupporting

confidence: 93%

Improving ICSI: A review from the spermatozoon perspective

Simopoulou

Gkoles

Bakas

et al. 2016

Systems Biology in Reproductive Medicine

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“…Zohdy et al [23] described crushing of testicular biopsies quickly frozen in liquid nitrogen as an alternative method to improve sperm recovery. In order to avoid the presence of heterogeneous testicular cell types in the final fraction for ICSI, some groups successfully included a density gradient centrifugation step [24, 25]. In NOA patients, Kamal et al [26] showed that in vitro microscopic dissection of the most dilated seminiferous tubules, if present, resulted in superior sperm recovery rates.…”

Section: Where Do We Come From?mentioning

confidence: 99%

“…After initial shredding and mincing, enzymatic digestion of the tissue can be performed followed by a real-time sperm vitality testing whenever appropriate. The combination of enzymatic treatment with density gradient centrifugation and exposure to theophylline in order to maximize sperm retrieval has been applied by Wöber et al [25]. A summary of procedures for processing and selection of surgically retrieved sperm is presented in Table 1.…”

Section: Where Are We Now?mentioning

confidence: 99%

Processing and selection of surgically-retrieved sperm for ICSI: a review

Verheyen¹,

Popović-Todorović²,

Tournaye³

2017

Basic Clin. Androl.

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Although the technique of intracytoplasmic sperm injection (ICSI) has been a revolution in the alleviation of male infertility, the use of testicular sperm for ICSI was a formerly unseen breakthrough in the treatment of the azoospermic man with primary testicular failure. At the clinical level, different procedures of testicular sperm retrieval (conventional TESE, micro-TESE, FNA/TESA, MESA, PESA) are being performed, the choice is mainly based on the cause of azoospermia (obstructive versus non-obstructive) and the surgeon’s skills. At the level of the IVF laboratory, mechanical procedures to harvest the sperm from the tissue may be combined with enzymatic treatment in order to increase the sperm recovery rates. A number of techniques have been developed for viable sperm selection in males with only immotile testicular sperm available. However, large, well-designed studies on the benefit and safety of one over the other technique are lacking. Despite all the available methods and combinations of laboratory procedures which have a common goal to maximize sperm recovery from testicular samples, a large proportion of NOA patients fail to father a genetically own child. Advanced technology application may improve recovery rates by detection of the testicular foci with active spermatogenesis and/or identification of the rare individual sperm in the testicular suspensions. On the other hand, in vitro spermatogenesis or sperm production from embryonic stem cells or induced pluripotent stem cells might be future options. The present review summarizes the available strategies which aim to maximize sperm recovery from surgically retrieved samples.

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“…Chemical reagents could activate viable spermatozoa that are immotile to recover their motion with high efficiency, especially in patients with extreme severe oligoasthenoteratozoospermia. A number of reagents, including pentoxifylline (PTX; Hattori et al., ; Kovacic, Vlaisavljevic, & Reljic, ; de Mendoza et al., ; Yildirim et al., ), theophylline (Ebner et al., , ; Wober et al., ) and papaverine (Terriou et al., ), enhance sperm motility by increasing intracellular cAMP and have been successfully used to select viable spermatozoa from patients with severe male infertility factor and result in healthy infants. Nevertheless, scientists are concerned about the embryotoxicities of these agents.…”

Section: Discussionmentioning

confidence: 99%

The clinical and neonatal outcomes after stimulation of immotile spermatozoa using SperMagic medium

Zhou

Zhang

et al. 2018

Andrologia

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To evaluate the efficiency and safety of SperMagic medium on stimulating the immotile spermatozoa in testicular sperm extraction (TESE) and absolute asthenozoospermia, 96 patients with TESE and 106 patients with absolute asthenozoospermia were enrolled in this study. The motile spermatozoa were detected in 47 TESE patients and 68 absolute asthenozoospermia and these patients were assigned to control group. The immotile spermatozoa in 49 TESE patients and 34 absolute asthenozoospermia were stimulated with SperMagic medium. Patients were treated by standard intracytoplasmic sperm injection (ICSI). There were no significant differences in fertilisation, cleavage, implantation, pregnancy, live birth and neonatal outcomes. SperMagic medium does not increase incidence of adverse neonatal outcomes and is a reliable tool for selection of viable spermatozoa in ICSI.

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A new method to process testicular sperm: combining enzymatic digestion, accumulation of spermatozoa, and stimulation of motility

Abstract: A comparison with a control group suggests that the present approach using standardized ready-to-use products is efficient and reliable. Presumably healthy live births further indicate the safety of the procedure.

Cited by 12 publications

References 34 publications

Improving ICSI: A review from the spermatozoon perspective

Improving ICSI: A review from the spermatozoon perspective

Processing and selection of surgically-retrieved sperm for ICSI: a review

The clinical and neonatal outcomes after stimulation of immotile spermatozoa using SperMagic medium

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