Improving ICSI: A review from the spermatozoon perspective

Simopoulou, Mara; Gkoles, Laertis; Bakas, Panagiotis; Giannelou, Polina; Kalampokas, Theodoros; Pantos, Konstantinos; Koutsilieris, Michael

doi:10.1080/19396368.2016.1229365

Cited by 48 publications

(36 citation statements)

References 115 publications

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“…Although in these seminal experiments the osmolarity of the nucleus and the egg at the time of injection is unknown, it is well described in the assisted reproduction field that the osmotic environment control is fundamental for successful fertilization. In intracytoplasmic sperm injection (ICSI) it is routine, to select the cells that perform best in a hyposmotic swelling test (HOST) which have been shown to lead to the formation of embryos with higher developmental potential 10 . On the other hand, it can be argued that even cells that have a low HOST score have the same fertility potential when the cell is delivered inside the cytoplasm and therefore HOST should be moot for ICSI cycles 10 .…”

Section: Introductionmentioning

confidence: 99%

Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation

Lima

May

Díaz-Colunga

et al. 2018

Sci Rep

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Chromatin structure is a major regulator of transcription and gene expression. Herein we explore the use of osmotic modulation to modify the chromatin structure and reprogram gene expression. In this study we use the extracellular osmotic pressure as a chromatin structure and transcriptional modulator. Hyposmotic modulation promotes chromatin loosening and induces changes in RNA polymerase II (Pol II) activity. The chromatin decondensation opens space for higher amounts of DNA engaged RNA Pol II. Hyposmotic modulation constitutes an alternative route to manipulate cell fate decisions. This technology was tested in model protocols of induced pluripotency and transdifferentiation in cells growing in suspension and adherent to substrates, CD34+ umbilical-cord-blood (UCB), fibroblasts and B-cells. The efficiency and kinetics of these cell fate modulation processes were improved by transient hyposmotic modulation of the cell environment.

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Section: Introductionmentioning

confidence: 99%

Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation

Lima

May

Díaz-Colunga

et al. 2018

Sci Rep

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“…Currently, the selection of sperm cells for ICSI is performed using the limited capabilities of relatively low‐magnification bright‐field microscopy (BFM), usually with ×200 to ×400 total magnification. This tool enables the embryologist to select motile sperm on the basis of basic morphological characteristics, such as the sperm head size .…”

Section: Introductionmentioning

confidence: 99%

Stain‐free interferometric phase microscopy correlation with DNA fragmentation stain in human spermatozoa

Barnea

Karako

Mirsky

et al. 2018

Journal of Biophotonics

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Acridine orange (AO) staining is used to diagnose DNA fragmentation status in sperm cells. Interferometric phase microscopy (IPM) is an optical imaging method based on digital holographic microscopy that provides quantitative morphological and refractive index imaging of cells in vitro without the need for staining. We have imaged sperm cells using stain-free IPM in order to estimate different cellular parameters, such as acrosome dry mass and size, in addition to an embryologist evaluation according to the World Health Organization (WHO)-2010 criteria. Following this, the same sperm cells were stained by AO, imaged using a fluorescence confocal microscope and assessed by the AO-emitted color, forming five DNA fragmentation groups. These DNA fragmentation groups were correlated with the embryologist-based classification and the IPM-based morphological parameters. Our results indicate on significant differences in the IPM-based parameters between groups with different fragmentation levels. Based on the validation with AO, we conclude that stain-free IPM images analyzed digitally may assist in selecting sperm cells with intact DNA prior to intracytoplasmic sperm injection. This information may potentially increase percentage of successful pregnancies.

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“…Within the in vitro fertilisation (IVF) set‐up and in the light of recent approaches to enhance male factor infertility management (Simopoulou et al., ), information on causes, biomarkers or treatment of infertility is valuable and may offer alternatives regarding the options available. Indeed, the value of predictive markers and factors within the IVF set‐up towards optimal selection and a successful IVF cycle is priceless.…”

Section: Introductionmentioning

confidence: 99%

Association between male Infertility and seminal plasma levels of growth hormone and insulin-like growth factor-1

et al. 2018

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Growth hormone (GH) and insulin-like growth factor 1 (IGF-1) have been proposed to play a pivotal role in male infertility due to their anabolic effects. The aim of this study was to investigate possible associations between seminal plasma levels of GH and IGF-1 and sperm parameters. Fifty men participated in this study. Semen analysis was performed, while cell-free seminal plasma was collected following sperm centrifugation. Seminal plasma concentrations of IGF-1 and GH were determined by enzyme-linked immunosorbent assay (ELISA). Due to the presence of asthenozoospermia in all participants who presented with abnormal sperm parameters, the participants were further subdivided into normal (group A), asthenozoospermic (group B) and asthenozoospermic plus at least one additional abnormal parameter (group C). A marginally nonsignificant statistical difference (p = 0.063) was revealed between the GH levels corresponding to the asthenozoospermic and the normal group with the latter presenting with higher GH levels. A statistically significant positive correlation (p < 0.05) was noted between levels of GH and IGF-1 in group C. The above relationship has also been observed in men with low sperm concentration, vitality, volume and abnormal morphology. These novel findings require further investigation in order for the biological significance of those associations to be clarified.

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Improving ICSI: A review from the spermatozoon perspective

Cited by 48 publications

References 115 publications

Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation

Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation

Stain‐free interferometric phase microscopy correlation with DNA fragmentation stain in human spermatozoa

Association between male Infertility and seminal plasma levels of growth hormone and insulin-like growth factor-1

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