The clinical and neonatal outcomes after stimulation of immotile spermatozoa using SperMagic medium

Gu, Yifan; Zhou, Qiong; Zhang, Shuo-Ping; Lu, Can‐Zhong; Gong, Fei; Shi, Yun; Lu, Guangxiu; Lin, Ge

doi:10.1111/and.13056

Cited by 8 publications

(7 citation statements)

References 27 publications

(29 reference statements)

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“…In one treatment cycle for couple 1 (F1: II-2), two treatment cycles for couple 2 (F2: II-2) and two treatment cycles for couple 3 (F3: II-1), biochemical pregnancies were confirmed following the transfer of embryos, but no fetal heartbeat was detected via ultrasound and the pregnancies ended in abortion. Thus, findings related to the three patients with acrosomal hypoplasia and other patients with completely immotile spermatozoa who displayed a clinical pregnancy rate over 60%21 indicate that CFAP65 mutants may impede ICSI outcomes.…”

Section: Resultsmentioning

confidence: 92%

Biallelic mutations in CFAP65 lead to severe asthenoteratospermia due to acrosome hypoplasia and flagellum malformations

Wang

Nie

et al. 2019

J Med Genet

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BackgroundThe genetic causes for most male infertility due to severe asthenozoospermia remain unclear.ObjectiveOur objective was to identify unknown genetic factors in 47 patients with severe asthenozoospermia from 45 unrelated Chinese families.MethodsWe performed whole exome sequencing of 47 individuals with severe asthenozoospermia from 45 unrelated families. Mutation screening was performed in a control cohort of 637 individuals, including 219 with oligoasthenospermia, 195 with non-obstructive azoospermia and 223 fertile controls. Ultrastructural and immunostaining analyses of patients’ spermatozoa were performed to characterise the effect of variants.ResultsOne homozygous non-sense mutation (NM_194302, c.G5341T:p.E1781X), two compound heterozygous mutations (c.C2284T:p.R762X and c.1751delC:p.P584fs) and two compound heterozygous mutations (c.5714_5721del:p.L1905fs and c.C3021A:p.N1007K) were identified in CFAP65 of three individuals with completely immotile spermatozoa, respectively. No biallelic deleterious variants of CFAP65 were detected in the control cohort of 637 individuals. Ultrastructural and immunostaining analyses of spermatozoa from two patients showed highly aberrant sperm morphology with severe defects such as acrosome hypoplasia, disruption of the mitochondrial sheath and absence of the central pair complex.ConclusionTo the best of our knowledge, we are the first to report that CFAP65 mutations may cause spermatozoa to be completely immotile.

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Section: Resultsmentioning

confidence: 92%

Biallelic mutations in CFAP65 lead to severe asthenoteratospermia due to acrosome hypoplasia and flagellum malformations

Wang

Nie

et al. 2019

J Med Genet

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“…If motile spermatozoa were found, the denuded oocytes were transferred to blank microdrops, and ICSI manipulation was performed according to standard protocol. 15 To ensure minimal manipulation exposure (<15 min per dish), no more than four mature oocytes were placed per ICSI dish during manipulation. The injected oocytes were cultured in G-IVF medium overnight before checking whether they were fertilized.…”

Section: Methodsmentioning

confidence: 99%

Influence of sperm morphology on pregnancy outcome and offspring in in vitro fertilization and intracytoplasmic sperm injection: a matched case-control study

et al. 2021

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Sperm morphology was once believed as one of the most predictive indicators of pregnancy outcome in assisted reproductive technology (ART). However, the impact of teratozoospermia on in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) outcomes and its offspring remains inconclusive. In order to evaluate the influence of teratozoospermia on pregnancy outcome and newborn status after IVF and ICSI, a retrospective study was conducted. This was a matched case-control study that included 2202 IVF cycles and 2574 ICSI cycles and was conducted at the Reproductive and Genetic Hospital of CITIC-Xiangya in Changsha, China, from June 2013 to June 2018. Patients were divided into two groups based on sperm morphology: teratozoospermia and normal sperm group. The pregnancy outcome and newborn outcome were analyzed. The results indicated that couples with teratozoospermia had a significantly lower optimal embryo rate compared to those with normal sperm morphology in IVF ( P = 0.007), while there were no statistically significant differences between the two groups in terms of the fertilization rate, cleavage rate, implantation rate, and pregnancy rate (all P > 0.05). Additionally, teratozoospermia was associated with lower infant birth weight in multiple births after IVF. With regard to ICSI, there was no significant difference in both pregnancy outcome and newborn outcome between the teratozoospermia and normal groups (both P > 0.05). Furthermore, no increase in the risk of birth defects occurred in the teratozoospermia group after IVF/ICSI. Consequently, we believe that teratozoospermia has limited predictive value for pregnancy outcomes in IVF/ICSI, and has little impact on the resulting offspring if multiple pregnancy is avoided.

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“…It exploits the chemical inducers of motility ( Figure 4 ), that belong to the class of phosphodiesterase inhibitors, such as pentoxifylline (PTF), dimethylxanthines and papaverines [ 90 ], allowing the motility reactivation of spermatozoa recovered from the testicular and epididymal level [ 91 ]. Using this motile fraction, it was possible to obtain fertilization, normal pregnancies, and an increase in birth rate through ICSI [ 92 , 93 , 94 ]. Some doubts about the technique concern the intrinsic toxicity of these molecules towards the genetic material, but results from studies suggest that in vitro treatment adequately improves motility of immotile sperm, without leading to acrosome reaction, DNA damage, and viability loss [ 95 , 96 ].…”

Section: Advanced Methodsmentioning

confidence: 99%

Sperm Selection for ICSI: Do We Have a Winner?

Baldini

Ferri

Baldini

et al. 2021

Cells

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In assisted reproductive technology (ART), the aim of sperm cells’ preparation is to select competent spermatozoa with the highest fertilization potential and in this context, the intracytoplasmic sperm injection (ICSI) represents the most applied technique for fertilization. This makes the process of identifying the perfect spermatozoa extremely important. A number of methods have now been developed to mimic some of the natural selection processes that exist in the female reproductive tract. Although many studies have been conducted to identify the election technique, many doubts and disagreements still remain. In this review, we will discuss all the sperm cell selection techniques currently available for ICSI, starting from the most basic methodologies and continuing with those techniques suitable for sperm cells with reduced motility. Furthermore, different techniques that exploit some sperm membrane characteristics and the most advanced strategy for sperm selection based on microfluidics, will be examined. Finally, a new sperm selection method based on a micro swim-up directly on the ICSI dish will be analyzed. Eventually, advantages and disadvantages of each technique will be debated, trying to draw reasonable conclusions on their efficacy in order to establish the gold standard method.

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The clinical and neonatal outcomes after stimulation of immotile spermatozoa using SperMagic medium

Cited by 8 publications

References 27 publications

Biallelic mutations in CFAP65 lead to severe asthenoteratospermia due to acrosome hypoplasia and flagellum malformations

Biallelic mutations in CFAP65 lead to severe asthenoteratospermia due to acrosome hypoplasia and flagellum malformations

Influence of sperm morphology on pregnancy outcome and offspring in in vitro fertilization and intracytoplasmic sperm injection: a matched case-control study

Sperm Selection for ICSI: Do We Have a Winner?

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