A new HPLC method developed for the analysis of oxygen heterocyclic compounds in<i>Citrus</i>essential oils

Russo, Marina; Torre, Germana; Carnovale, Caterina; Bonaccorsi, Ivana; Mondello, Luigi; Dugo, Paola

doi:10.1080/10412905.2012.659523

Cited by 31 publications

(34 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Under the optimum condition, the LODs and LOQs of coumarin and its derivatives were in the range of 0.5-1.7 µg·kg −1 and 1.7-5.2 µg·kg −1 , respectively. It is worth mentioning that the LODs of coumarins determined by previous published methods, such as GC-MS [34,35] and HPLC [24,25,36] were around 10-100 µg·kg −1 , which is much higher than the LODs determined by the HPLC-MS/MS method developed in this study (0.5 µg·kg −1 ). The sensitivity of this method was highly improved over conventional GC methods and the lower LOD makes it possible to determine trace amounts of coumarin compounds in real samples.…”

Section: Calibration and Sensitivitycontrasting

confidence: 64%

“…So far, a variety of analytical methods based on GC [20,21], GC-MS [22], HPLC [23][24][25][26][27], and LC-MS [28,29] have been developed to analyze coumarins, but the low accuracy, high organic reagent consumption and long analysis times of these conventional methods are primary obstacles because of the complex matrix effects of coumarins. In recent years, tandem mass spectrometry (MS/MS) has been reported to provide a much higher degree of assurance than the single stage mass spectrometry technique when determining analytes in many complex matrix [30].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

et al. 2016

View full text Add to dashboard Cite

Abstract:In this paper an analytical method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) for the determination of coumarin and its derivatives in tobacco products was developed. The MS/MS fragmentation pathways of the eight coumarins were elucidated. The new analytical method was defined based on two main axes, an extraction procedure with acetonitrile and analyte detection performed by HPLC-MS/MS in electron impact mode. The excellent selectivity and sensitivity achieved in multiple reaction monitoring (MRM) mode allowed satisfactory confirmation and quantitation for the coumarin flavor additives. Under the optimized gradient elution conditions, it took only 4.5 min to separate all eight coumarins. Good linearity for all the analytes were confirmed by the correlation coefficient r 2 , ranging from 0.9987 to 0.9996. The limits of detection (LODs) and limits of quantitation (LOQs) of these compounds were in the range of 0.5-1.7 µg/kg and 1.7-5.2 µg/kg, respectively. The average recoveries at three spiked levels (LOQ, 1.5LOQ, 2LOQ) were all in the range of 69.6%-95.1% with RSDs (n = 6) lower than 5.3%. The method of HPLC-MS/MS developed in this study was initially applied to the research of coumarin flavor additives in tobacco products collected from the located market in Beijing from China and proved to be accurate, sensitive, convenient and practical.

show abstract

Section: Calibration and Sensitivitycontrasting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Coumarin was purchased from Fluka (Milan, Italy). Nobiletin and tangeretin have been previously isolated in our laboratory …”

Section: Methodsmentioning

confidence: 99%

“…In order to quantify the PMF content in the cold‐pressed bergamot oils, calibration curves have been constructed by using each single standard. Five different concentrations of each component, in the range between 100 and 0.1 mg/l, were prepared by diluting a stock solution of about 1000 mg/l, using methanol as a solvent, and analysed five consecutive times by HPLC under the same chromatographic conditions optimized for bergamot oil, as previously reported …”

Section: Methodsmentioning

confidence: 99%

Determination of new bioflavonoids in bergamot (Citrus bergamia) peel oil by liquid chromatography coupled to tandem ion trap–time‐of‐flight mass spectrometry

Donato

Bonaccorsi

Russo

et al. 2013

Flavour & Fragrance J

Self Cite

View full text Add to dashboard Cite

A new investigation on manually extracted samples of bergamot (Citrus bergamia) essential oil was performed by high‐performance liquid chromatography coupled with an ion trap–time‐ of‐flight mass spectrometer. The study aimed to define new quality parameters for the evaluation of bergamot essential oils based on the composition of bioflavonoid. Polymethoxylated flavones were identified in manually cold‐pressed bergamot (C. bergamia) essential oil from fruits of three different cultivars (‘Femminello’, ‘Castagnaro’ and ‘Fantastico’) harvested in southern Italy. The separation of the oxygen heterocyclic compounds was optimized by reversed‐phase liquid chromatography on a partially porous C18 column under gradient elution. Afterwards, calibration curves were obtained for the quantitative assay using photodiode array detection, while a hybrid ion trap–time‐of‐flight mass spectrometer was used for structure elucidation, by tandem mass experiments. The methods applied for the identification and the quantitative determination were validated. The results obtained on samples of secure origin prove that the profile of the non‐volatile fraction of bergamot peel oil is characterized, in addition to the well known coumarines and psoralens, also by four polymethoxylated flavones, namely sinensetin, nobiletin, tetra‐O‐methyl‐scutellarein, and tangeretin. In particular, the identification and quantitative determination of nobiletin and tangeretin are hereby reported, for the first time, in cold‐pressed bergamot essential oil. All the essential oil manually extracted from fruits of the three most common cultivars of C. bergamia contain variable amounts of the four polymethoxyflavones, with the cv. ‘Femminello’ being the one with the highest total content. Copyright © 2013 John Wiley & Sons, Ltd.

show abstract

“…High‐performance liquid chromatography with diode array detection (HPLC‐DAD) is a commonly used technique for the analysis of furocoumarins. Chromatographic separation can be performed on both normal and reversed phase but recent works are generally performed on C 18 reversed phases, with detection carried out at 310–315 nm . The substitution pattern of the compounds can lead to different absorbance spectra and can help differentiate the compounds .…”

Section: Introductionmentioning

confidence: 99%

Quantification of furocoumarins in hydroalcoholic fragrances by a liquid chromatography–high resolution/accurate mass method

Corbi¹,

Pérès²,

David³

2014

Flavour & Fragrance J

View full text Add to dashboard Cite

Furocoumarins are secondary metabolites that occur in various plant species used as ingredients in fragrances and cosmetics. Those compounds have received an increased interest in those matrices since they are known to induce photosensitization and to have potential carcinogenic, mutagenic and reprotoxic effects. To date, there are no restricted limits regarding these compounds; however, some committees proposed following seven targeted compounds at low level concentration in finished cosmetic products (≤1 mg/l). This implies the need for a robust and specific analytical method to control and determine their concentrations in essential oils or more complex matrices such as fragrances. An innovative RP‐HPLC high‐resolution/accurate‐mass MS method based on Orbitrap technology was developed to quantify seven furocoumarins in fragrances in the range 0.01–1 mg/l. This method was developed and validated according to accuracy profile methodology. Accurate mass and high resolution provides a robust and highly specific approach to quantify the compounds and minimize tedious sample preparation. The method was finally used to quantify more than 50 perfumes (eaux de toilette, eaux de parfum, extraits). Copyright © 2014 John Wiley & Sons, Ltd.

show abstract

A new HPLC method developed for the analysis of oxygen heterocyclic compounds inCitrusessential oils

Cited by 31 publications

References 26 publications

Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

Determination of new bioflavonoids in bergamot (Citrus bergamia) peel oil by liquid chromatography coupled to tandem ion trap–time‐of‐flight mass spectrometry

Quantification of furocoumarins in hydroalcoholic fragrances by a liquid chromatography–high resolution/accurate mass method

Contact Info

Product

Resources

About