Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

Abstract

Abstract:In this paper an analytical method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) for the determination of coumarin and its derivatives in tobacco products was developed. The MS/MS fragmentation pathways of the eight coumarins were elucidated. The new analytical method was defined based on two main axes, an extraction procedure with acetonitrile and analyte detection performed by HPLC-MS/MS in electron impact mode. The excellent selectivity and sensitivity achieved in multiple reaction monitoring (MRM) mode allowed satisfactory confirmation and quantitation for the coumarin flavor additives. Under the optimized gradient elution conditions, it took only 4.5 min to separate all eight coumarins. Good linearity for all the analytes were confirmed by the correlation coefficient r 2 , ranging from 0.9987 to 0.9996. The limits of detection (LODs) and limits of quantitation (LOQs) of these compounds were in the range of 0.5-1.7 µg/kg and 1.7-5.2 µg/kg, respectively. The average recoveries at three spiked levels (LOQ, 1.5LOQ, 2LOQ) were all in the range of 69.6%-95.1% with RSDs (n = 6) lower than 5.3%. The method of HPLC-MS/MS developed in this study was initially applied to the research of coumarin flavor additives in tobacco products collected from the located market in Beijing from China and proved to be accurate, sensitive, convenient and practical.

Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors

“…UV-Vis spectra were recorded using a photodiode array detector (PDA) (Shimadzu, Kyoto, Japan). The LC-MS protocol applied was developed based on previous studies [49,50,51,52]. The low aqueous solubility of all compounds tested necessitated the application of a relatively strong gradient.…”

LC-ESI-QTOF-MS/MS Characterisation of Phenolics in Herbal Tea Infusion and Their Antioxidant Potential

“…A total of 9 phenolic compounds classified as other phenolic compounds were found in the samples. [94]. Previously coumarin and its derivatives have been reported to be associated with beneficial health effects such as reducing inflammation and risk of cancer, which is thought to be due to their antioxidant properties [95].…”

Development of a colloidal gold immunoassay for the detection of four eugenol compounds in water

“…At present, the detection of eugenol compounds is mainly based on instrumental analysis, such as gas chromatography-mass spectrometer (Huang et al, 2017;Liu, Ai, Li, Li, & Yang, 2018), high-performance liquid chromatography (Thyagaraj et al, 2013), liquid chromatography-tandem mass spectrometry (Ren et al, 2016), ultra-high-performance liquid chromatography-tandem mass spectrometry (Sun, Gao, & Lian, 2017;Zhao et al, 2017), solidphase extraction (Ke et al, 2016) and various instruments (Li, Liu, Wang, Wu, & Liu, 2016;Li et al, 2015;Liang et al, 2018). Although these methods for determining eugenol residue levels are highly sensitive, the procedure for sample pretreatment is complicated, the equipment is expensive, a professional operator is required, and samples must be analyzed in the laboratory.…”