A new gene superfamily of pathogen-response (repat) genes in Lepidoptera: Classification and expression analysis

Comprehensive Analysis of Gene Expression Profiles of the Beet Armyworm Spodoptera exigua Larvae Challenged with Bacillus thuringiensis Vip3Aa Toxin

“…The general up-regulation of the repat unigenes from class α together with repat45 , and the down-regulation of class ß repat members 42 and 43, coincide with the transcriptional profiles reported in S. exigua treated with Cry1C by Navarro-Cerrillo et al [54]. Although the role of REPAT proteins remains unclear, the large number of repat members regulated, their homology to transcriptional activators in other species of Lepidoptera, and their ability to form heterodimers and translocate into the nucleus [78] seems to point to a possible role in the transcriptional activation of several sets of genes in response to physiological changes in the midgut produced by Vip3A or Cry1C intoxication.…”

“…Recently, up to 46 members of this family have been reported in S. exigua , and homologous sequences have been found in other species [54]. After Vip3Aa feeding, a broad response of repat genes was detected.…”

“…Almost all repat members that were significantly regulated belonged to REPAT class α (groups I and II), and only five of them belonged to REPAT class ß (to groups III, IV, and VI) [54]. The general up-regulation of the repat unigenes from class α together with repat45 , and the down-regulation of class ß repat members 42 and 43, coincide with the transcriptional profiles reported in S. exigua treated with Cry1C by Navarro-Cerrillo et al [54].…”

“…The suitability of the primers was further assessed in the qRT-PCR working conditions. The ATP synthase subunit C house-keeping gene was used as an internal control for normalization of the samples [29,33,44,54]. The cDNA was synthesized from 1 µg of RNA treated with DNase I (Invitrogen, Life Technologies Corporation, CA, USA) by reverse transcription using oligo-d(T) primer and SuperScript II Reverse Transcriptase (Invitrogen) according to manufacturer protocols.…”

Specific genes related to nucleopolyhedrovirus in Bombyx mori susceptible and near‐isogenic resistant strains through comparative transcriptome analysis

“…In this study, MBF2‐10 was specifically expressed in the midgut, and its expression level was significantly upregulated in 871C upon viral infection. The MBF2 family activates transcription in combination with transcription factor IIA, and has a closer relationship with the REsponse to PAThogens (REPAT) gene, which has been reported to be involved in the immune response against the Bacillus thuringiensis virus or baculovirus in Spodoptera exigua (Li et al ., ; Liu et al ., ; Navarro‐Cerrillo et al ., ; Zhou et al ., ). Moreover, the amino acid sequence and protein structure of MBF2‐10 differed between 871 and 871C, which affected the inhibition of viral infection.…”