Midgut de novo transcriptome analysis and gene expression profiling of Spodoptera exigua larvae exposed with sublethal concentrations of Cry1Ca protein

Toxicological and molecular profiling of insecticide resistance in a Brazilian strain of fall armyworm resistant to Bt Cry1 proteins

“… 66 , 71 , 72 In S. exigua , CYP4S9 , CYP6AB31 , CYP6AE47 and CYP9A were upregulated after exposure to Cy1Ca and a similar response was observed in insects exposed to insecticides ( lambda ‐cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb). 66 , 73 A few studies have shown that CE is related to Cry resistance in P. xylostella , O. furnacalis and C. medinalis 74 , 75 , 76 and GST were downregulated in O. furnacalis to Cry1Ab and C. medinalis to Cry1Ac, Cry1Ab and Cry1C. 76 , 77 In our studies, we have not checked the expression pattern after the exposure to Bt proteins.…”

“…The CYPs have been observed to respond to sublethal doses of Cry toxins in different insect species, such as Choristoneura fumiferana , Manduca sexta , Ostrinia nubilalis and also S. exigua 66, 71, 72 . In S. exigua , CYP4S9 , CYP6AB31 , CYP6AE47 and CYP9A were upregulated after exposure to Cy1Ca and a similar response was observed in insects exposed to insecticides ( lambda ‐cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb) 66, 73 . A few studies have shown that CE is related to Cry resistance in P. xylostella , O. furnacalis and C. medinalis 74–76 and GST were downregulated in O. furnacalis to Cry1Ab and C. medinalis to Cry1Ac, Cry1Ab and Cry1C.…”

“…Recently, a midgut transcriptome analysis was performed with S. exigua exposed to sublethal doses of Cry1Ca and among the DEG, some differences in expression of P450, CE and GST genes were observed besides Bt‐related genes, such as ABC transporters 66 . Moreover, cross‐resistance between pyrethroids and Cry1Ac has been reported in the diamondback moth, Plutella xylostella .…”

“…Recently, a midgut transcriptome analysis was performed with S. exigua exposed to sublethal doses of Cry1Ca and among the DEG, some differences in expression of P450, CE and GST genes were observed besides Bt‐related genes, such as ABC transporters. 66 Moreover, cross‐resistance between pyrethroids and Cry1Ac has been reported in the diamondback moth, Plutella xylostella . Genetic studies suggest that possible interactions between esterases and Bt protein and/or indirect triggering of a defense metabolic pathway are involved and genetically linked at a common locus.…”

A Novel Reference for Bt-Resistance Mechanism in Plutella xylostella Based on Analysis of the Midgut Transcriptomes

“…The total economic cost of its damage and management worldwide is more than US $4-5 billion per year [1], primarily due to strong resistance to multifarious Many studies have been conducted to examine transcriptome of P. xylostella, such as tissue-specific transcriptome analysis of a Bt-resistant strain [49]. Comparative studies were also reported on transcriptomic differences between Bt-susceptible and -resistant strains, although the focus is on different Bt toxins [43,[45][46][47]50,51]. Moreover, transcriptome analyses of different insecticides and resistant strains have been reported [52][53][54][55].…”