Specific genes related to nucleopolyhedrovirus in Bombyx mori susceptible and near‐isogenic resistant strains through comparative transcriptome analysis

Abstract

Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the primary pathogens that causes severe economic losses to sericulture. Comparative transcriptomics analysis has been widely applied to explore the antiviral mechanism in resistant strains. Here, to identify genes involved in BmNPV infection, we identified differentially expressed genes (DEGs) and performed weighted gene co‐expression network analysis (WGCNA) between two Bombyx mori strains: strain 871 (susceptible to BmNPV infection) and the near‐isogenic strain 871C (resistant to BmNPV). Our results showed that 400 genes were associated with resistance in strain 871C, and 76 genes were related to susceptibility in strain 871. In addition, the correlation analysis of DEGs and WGCNA showed that 40 genes related to resistance were highly expressed in the resistant strain. Among them, gene BGIBMGA004291 was the most noticeable. We further identified the effect of gene BGIBMGA004291, which encoded a multiprotein bridge factor 2 (MBF2) family member (MBF2‐10), on viral infection in cells. Our data suggested that MBF2‐10 inhibited viral infection. Taken together, this study showed specific module trait correlations related to viral infection in strains 871 and 871C, and we identified a resistance‐related gene. These findings suggested promising candidate genes with antiviral activity, aiding in the analysis of the antiviral molecular mechanisms in resistant strains.

Characterization of Dendrolimus houi Lajonquiere (Lepidoptera: Lasiocampidae) Transcriptome across All Life Stages

“…Transcriptomics and de novo transcriptome reconstruction is a robust method to characterize these mechanisms across developmental stages of D. houi. Technically, the high-throughput next generation sequencing technology (NGS) has greatly promoted the application of insect transcriptomics [9][10][11][12][13][14], mostly focusing on their growth and development, classification, toxicology, interaction between insects and host plants and even non-coding RNA. For instance, transcriptomic data of Adelphocoris suturalis and D. punctatus across different stages was obtained using NGS techniques to define the gene expression related to the development of insects [15][16][17] and that of Spodoptera frugiperda was sequenced to reveal the mechanism of antivirus resistance [18].…”

Proteomics Analysis to Explore the Resistance Genes of Silkworm to Bombyx mori Nuclear Polyhedrosis Virus

“…Recently, Huakang series [6][7][8] bred by the Sericulture Research Institute, Chinese Academy of Agriculture Science, Yunkang No. 1 [9,10] bred by the Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, and Guican N2 [11] bred by Guangxi Sericulture Science Research Institute have a remarkable effect on resistance to BmNPV. Among them, Huakang and Yunkang 1 were bred by hybridization, backcrossing, selfing, and other breeding methods, using the highly BmNPV-resistant silkworm variety N with high tolerance to BmNPV as the female parent.…”

Comparison of the signaling pathways of wing dimorphism regulated by biotic and abiotic stress in the brown planthopper

“…, 2021), and antiviral activity (Chen et al. , 2019a). In this study, early 4th instar nymphs of ‘laboratory population’ and ‘field population’ under BS and AS were collected.…”

“…However, comparative studies on gene expression in BPH under 2 types of stress, BS and AS, are scarce. Weighted gene coexpression network analysis (WGCNA, Langfelder & Horvath, 2008) can link gene expression patterns to biological phenotypes and has become a powerful strategy for the identification of key candidate genes associated with specific phenotypic traits, such as cold resistance Zhang et al, 2021a), insecticide resistance (Tian et al, 2021), aggressive behavior (Rao et al, 2021), and antiviral activity (Chen et al, 2019a). In this study, early 4th instar nymphs of 'laboratory population' and 'field population' under BS and AS were collected.…”