A new enzyme immunoassay system suitable for field use and its application in a snake venom detection kit

Chandler, Howard M.; Hurrell, John G.R.

doi:10.1016/0009-8981(82)90062-6

Cited by 51 publications

(9 citation statements)

References 6 publications

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“…The use of glass capillary tubes and urease-anti body conjugates can enable EIA to be conducted out side well-equipped laboratories by people with little experience in immunoassay techniques [6]. The results presented in this paper confirm the feasibility of a semiquantitative EIA, of potential use in physicians' or allergists' consulting rooms, for the rapid detection of elevated/depressed levels of human IgE.…”

Section: Discussionsupporting

confidence: 63%

“…Urease substrate for use in the capillary tube assays was pre pared as described by Chandler and Hurrell [6],…”

Section: Urease Substratementioning

confidence: 99%

“…We have previously reported the successful devel opment of a rapid EIA procedure, suitable for field use, using glass capillary tubes as a solid support and antibodies labelled with the enzyme urease [6], In that procedure, for the rapid detection and identification of snake venom in specimens from suspected snake bite victims, a quantitative result was not required. In this paper the modification of that technique to en able semiquantitative estimation of total IgE levels in human blood or serum is described.…”

Section: Introductionmentioning

confidence: 99%

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Monoclonal Hybridoma Antibodies against Human IgE and Their Use in a Rapid and Sensitive Enzyme Immunoassay for the Semiquantitative Assessment of Total IgE Levels in Human Blood

Chandler¹,

Coulter²,

Healey³

et al. 1983

Int Arch Allergy Immunol

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A simple semiquantitative enzyme immunoassay (EIA) for the rapid estimation of IgE levels in specimens of human blood, plasma or serum is described. The test requires little labour input and does not require highly trained personnel or instrumentation. By using two monoclonal antibodies of different anti-IgE specificities it is possible, with a single incubation of 20 min at ambient temperature, to detect elevated IgE levels (≥ 33 IU/ml) within a total test time of 25 min, and low levels of IgE (≤ 10 IU/ml) within 35 min. For diagnosis of elevated/normal IgE levels only, a single incubation of 10 min. at ambient temperature may be used with a total test time of less than 20 min. The EIA system utilizes glass capillary tubes and urease-labelled antibodies, a system that has proven satisfactory in other applications.

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Section: Discussionsupporting

confidence: 63%

“…Urease substrate for use in the capillary tube assays was pre pared as described by Chandler and Hurrell [6],…”

Section: Urease Substratementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Monoclonal Hybridoma Antibodies against Human IgE and Their Use in a Rapid and Sensitive Enzyme Immunoassay for the Semiquantitative Assessment of Total IgE Levels in Human Blood

Chandler¹,

Coulter²,

Healey³

et al. 1983

Int Arch Allergy Immunol

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show abstract

“…4 Laboratory diagnostic techniques, notably enzyme immunoassays, have been developed to detect venom antigens in blood, wound swabs, wound aspirate, and other body fluids, [6][7][8][9][10] but commercial venom detection kits are marketed only in Australia. 11 An alternative approach is to identify distinctive clinical syndromes associated with bites by individual species. This type of syndromic classification of snake bites will enable identification of snake bites without relying solely on the live or killed snake or victim's description of the snake.…”

Section: Introductionmentioning

confidence: 99%

Syndromic Approach to Treatment of Snake Bite in Sri Lanka Based on Results of a Prospective National Hospital-Based Survey of Patients Envenomed by Identified Snakes

Ariaratnam¹,

Sheriff²,

Arambepola³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

103

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Abstract. Of 860 snakes brought to 10 hospitals in Sri Lanka with the patients they had bitten, 762 (89%) were venomous. Russell's vipers ( Daboia russelii ) and hump-nosed pit vipers ( Hypnale hypnale ) were the most numerous and H. hypnale was the most widely distributed. Fifty-one (6%) were misidentified by hospital staff, causing inappropriate antivenom treatment of 13 patients. Distinctive clinical syndromes were identified to aid species diagnosis in most cases of snake bite in Sri Lanka where the biting species is unknown. Diagnostic sensitivities and specificities of these syndromes for envenoming were 78% and 96% by Naja naja , 66% and 100% by Bungarus caeruleus , 14% and 100% by Daboia russelii , and 10% and 97% by Hypnale hypnale , respectively. Although only polyspecific antivenoms are used in Sri Lanka, species diagnosis remains important to anticipate life-threatening complications such as local necrosis, hemorrhage and renal and respiratory failure and to identify likely victims of envenoming by H. hypnale who will not benefit from existing antivenoms. The technique of hospital-based collection, labeling and preservation of dead snakes brought by bitten patients is recommended for rapid assessment of a country's medically-important herpetofauna.

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“…Urease as marker enzyme was tested successfully by and Chandler and Hurell (1982). + GOD/POD systems are much more insensitive in short-time measuring than HRP as marker enzyme.…”

Section: Chromogens For Ureasementioning

confidence: 99%

Chromogenic Substrates for Enzyme Immunoassay

Porstmann

1988

Nonisotopic Immunoassay

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A new enzyme immunoassay system suitable for field use and its application in a snake venom detection kit

Cited by 51 publications

References 6 publications

Monoclonal Hybridoma Antibodies against Human IgE and Their Use in a Rapid and Sensitive Enzyme Immunoassay for the Semiquantitative Assessment of Total IgE Levels in Human Blood

Monoclonal Hybridoma Antibodies against Human IgE and Their Use in a Rapid and Sensitive Enzyme Immunoassay for the Semiquantitative Assessment of Total IgE Levels in Human Blood

Syndromic Approach to Treatment of Snake Bite in Sri Lanka Based on Results of a Prospective National Hospital-Based Survey of Patients Envenomed by Identified Snakes

Chromogenic Substrates for Enzyme Immunoassay

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