1964
DOI: 10.1017/s0022172400039735
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A new enrichment medium for salmonellae

Abstract: A new enrichment medium for the isolation of salmonella types from faeces is described.The inhibitory substances for coliform organisms and enterococci in this medium are magnesium chloride and sodium hydrogen selenite.The new medium was found in routine diagnostic work to be superior to the Mueller–Kauffmann medium in the isolation ofSalmonella typhiand only a little inferior in the isolation of other salmonella types.When used in addition to the standard media the new medium increases the number of positive … Show more

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Cited by 6 publications
(3 citation statements)
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“…Rappaport & Konforti (1958) noted that the inhibitory action of malachite green could be selectively modified by certain bivalent cations to inhibit the growth of non-pathogenic Gram-negative microorganisms, while allowing the growth of certain salmonellas. More recently, Banic (1964) and Zajc-Satler & Bani6 (1965) used a selective combination of magnesium chloride and sodium hydrogen selenite. This enrichment medium, although efficient, was inferior to tetrathionate infusion broth.…”
Section: Introductionmentioning
confidence: 99%
“…Rappaport & Konforti (1958) noted that the inhibitory action of malachite green could be selectively modified by certain bivalent cations to inhibit the growth of non-pathogenic Gram-negative microorganisms, while allowing the growth of certain salmonellas. More recently, Banic (1964) and Zajc-Satler & Bani6 (1965) used a selective combination of magnesium chloride and sodium hydrogen selenite. This enrichment medium, although efficient, was inferior to tetrathionate infusion broth.…”
Section: Introductionmentioning
confidence: 99%
“…The MS medium, which is composed of nutrient broth Difco + 02% yeast extract Difco + 005% sodium hydrogen selenite (NaHSeO3) (Merck) + 3-4% MgC12, was prepared as described in a previous paper (Banic, 1963). It should be mentioned that the stock solution of 40% MgCl2 used in the preparation of the MM medium was made up according to the method of Rappaport et al (1956) so that 100 g. of MgCI2 (the whole content of a newly-opened bottle) was dissolved in 250 ml.…”
mentioning
confidence: 99%
“…Recently, Vassiliadis et al (1976) demonstrated a slight superiority of enrichment of minced meat in Rappaport broth in comparison to enrichment in tetrathionate broth. Banic (1964) described a magnesium selenite medium which was superior to tetrathionate broth for isolating S. typhi but unsuccessful in comparison to other enrichment broths for detecting other solmonelloe. Two enrichment media, strontium chloride and strontium selenite broths, which also incorporated specific ions to achieve a selective environment were introduced by Iveson and MacKay-Scoilay (1969).…”
Section: Selective Enrichmentmentioning
confidence: 99%