Efficacy of different selective media for the isolation of salmonellae from faeces

Abstract

SYNOPSIS Comparative results obtained in isolating salmonellae from faeces by the use of tetrathionate broth, MS, and MM enrichment medium are given. The best results were obtained by tetrathionate broth prepared from infusion broth. The MS as well as the MM medium increased the total number of positive results.There are many efficient selective media for the isolation of salmonellae, but none of them is as efficient as desired. To obtain the best results many authors recommend the use of two or three different enrichment media in addition to the direct plates (Sen, 1964; Guinde and Kampelmacher, 1962;Taylor, Buttiaux, and Catsaras, 1963). The purpose of this study was to compare the efficacy of the MM (Rappaport, Konforti, and Navon, 1956) and MS (Banic, 1963) The MS medium, which is composed of nutrient broth Difco + 02% yeast extract Difco + 005% sodium hydrogen selenite (NaHSeO3) (Merck) + 3-4% MgC12, was prepared as described in a previous paper (Banic, 1963). It

A comparative trial of Rappaport enrichment medium for the isolation of Salmonellae from faeces

“…Iveson, Kovacs, and Laurie (1964), working with infected desiccated coconut, and Anderson and Kennedy (1965), using pure cultures, also reported the superiority of Rappaport's medium. The results of other workers differ: Sen (1964) obtained a higher Salmonellae yield in selenite broth, and Zajc-Satler and Banic (1965) found increased isolations in tetrathionate medium prepared from infusion broth. However, both authors commented on Rappaport as being an efficient isolation medium.…”