A New Cytotoxicβ-Carboline Alkaloid fromGalianthe thalictroides

Abstract

A cytotoxicity-guided fractionation of the roots of Galianthe thalictroides afforded a new β-carboline alkaloid, 1-(hydroxymethyl)-3-(2-hydroxypropan-2-yl)-2-(5-methoxy-9H- β-carbolin-1-yl)cyclopentanol, which exhibited strong cytotoxic activity against three human cancer cell lines (MCF-7, 786-0, and UACC62). Its structure was established on the basis of 1D- and 2D-NMR spectroscopic techniques supported by HRMS data.

Assessment of the in vitro and in vivo genotoxicity of extracts and indole monoterpene alkaloid from the roots of Galianthe thalictroides (Rubiaceae)

“…These data agree with the results of Figueiredo et al (2011), in which the same CHCl 3 phase exhibited a high cytotoxic activity against MCF-7 Table 5 Frequency of mutant spots in the wings of marked trans-heterozygous descendants (mwh/flr 3 ) of D. melanogaster using the standard crossing (ST) and high bioactivation cross (HB) after chronic treatment with the ethanol extract (EtOH) of G. thalictroides. Frei and Würgler (1988): +, positive; À, negative; i, inconclusive; m: multiplication factor for the assessment of significantly negative results; % R (Recombination).…”

“…This difference in toxicity should be attributed to the presence of other metabolites in the bioactive CHCl 3 phase. In a previous work, differences between the cytotoxic behavior of this phase and alkaloid 1 were also observed against MCF-7 cell lines (Figueiredo et al, 2011). The recombination events induced by the CHCl 3 phase in flies from the cross were however not observed for alkaloid 1, and therefore the effects caused by this compound are shown to be exclusively related to its mutagenic activity, and since they were proportional to the concentrations applied, these results were indicative of a dose-response relationship.…”

“…These extracts were filtered through paper filters and diluted immediately before use in sterile distilled water, to obtain the concentrations of 20 mg/mL, 10 mg/mL, 5 mg/mL and 2.5 mg/mL. The EtOH extract from the roots and the CHCl 3 phase from partition of the EtOH extract, as well as the indole-monoterpene alkaloid 1 were obtained according to Figueiredo et al (2011). These samples were initially diluted in 1% Tween 80, 3% ethanol and sterile distilled water, to obtain the concentrations used in the SMART assay: EtOH extract (0.5, 1.0 and 2.0 mg/mL), CHCl 3 phase (0.2, 0.4, 0.8 and 1.6 mg/ mL), alkaloid 1 (0.2, 0.4, 0.8 and 1.6 mg/mL).…”

“…In a previous work, a cytotoxicity-guided fractionation of the bioactive CHCl 3 phase obtained from partition of the EOH extract from the roots of this plant led to the isolation of the cytotoxic monoterpene indole alkaloid 1 (Fig. 1) as its major component (Figueiredo et al, 2011).…”

Obtenção de farinha de inhame para elaboração de barra de cereal como suplemento alimentar e funcional

“…O valor calórico (kcal/100 g) foi calculado pela soma dos resultados da multiplicação dos fatores gerais de farinha de inhame e o liofilizado do tubérculo foram diluídos em Dimetilsulfóxido (DMSO) na concentração de 0,1 g/mL. Para adicioná-los nas células, foi realizada diluição seriada em meio de cultura, sendo utilizadas as concentrações de 0,25, 2,5, 25 e 250 μg/ml em triplicata(FIGUEIREDO et al, 2011).No presente estudos teores de proteína encontrados na farinha de inhame corroboram com os encontrados por PAULA (2012) em estudo nos inhames da espécie D.alata e inhame Flórida 6,35% e no inhame Caramujo 5,55% de proteína em base seca, o que representa valores semelhantes. CARACTERIZAÇÃO DA BARRA DE CEREAL O rendimento das barras de cereal foi de 95,26%, depois de assado.…”