A new colorimetric method to quantify β-1,3-1,6-glucans in comparison with total β-1,3-glucans in edible mushrooms

In-vitro antimicrobial activity and complement/macrophage stimulating effects of a hot-water extract from mycelium of the oyster mushroom Pleurotus sp.

“…In a previous work, we reported that the 1.5% of Pleurotus mycelial extract dry mass consists of β-1,3-1,6-glucans . This content was lower compared to that of P. ostreatus mycelia (2.97%) but higher than those referred on P. pulmonarius (0.41%) (Nitschke et al, 2011). It has been reported that complement components would recognize the primary as well as ultrastructure of 1,3-β-Dglucans, and it may be complementary serving as the activator of cell defense systems.…”

Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit

“…β-glucan from L. edodes is highly branched and production is high when compared to other mushrooms [2]. …”

“…Quantification of (1,3→1,6)-β-glucan content in Shiitake extracts was performed by the acid/basic fractions method described by Mölleken et al [2] with slight modifications. Briefly, samples were resuspended in citric buffer solution (0.5M NaOH, KOH 1M 6M HCl, 1M HCl, 0.55 M HCl, 6M KOH buffer 0.2M citric acid/NaOH pH = 7.00) and staining solution (0.08 grams of Congo Red in 100ml of citric buffer).…”

“…Among mushrooms Shiitake ( Lentinula edodes ) produces high quantities of branched β (1–3)(1–6)-D-glucan (Lentinan) [2,3]. Interaction of β-glucans with different cellular receptors inducing intracellular signalling may activate different profiles of the immune response [1,4,5].…”

Base-soluble polysaccharides from sclerotia of Inonotus obliquus

“…This indicated that the polymer macromolecules had a twisted configuration [8]. The quantitative content of E-glucans was determined by the method of Nitschke et al [9] and was 3.12 ± 0.09% (of the air-dried raw material mass). .94 (C-6)], which confirmed the D-configuration of the glucopyranose (shift of the C-1 resonance to strong field) and (1o3)-bonds in the main polymer chain (shift of the C-3 resonance to weak field) [10,11].…”