A new collagenase enzyme of the marine sponge pathogen Pseudoalteromonas agarivorans NW4327 is uniquely linked with a TonB dependent receptor

Proteases from the marine bacteria in the genus Pseudoalteromonas: diversity, characteristics, ecological roles, and application potentials

“…These data encourage the potential use of SM9913 extracellular protease in preparing bioactive oligopeptides from fish skin (Chen et al 2017). The collagenases produced Lee et al (2000Lee et al ( , 2002 Two collagenases P. agarivorans NW4327 Rhopaloeides odorabile Degrading skeletal fibers Bhattacharya et al (2018Bhattacharya et al ( , 2019 Zhao et al (2012b) by Pseudoalteromonas sp. SJN2 can hydrolyze marine collagens extracted from fish by-products, Spanish mackerel bone, seabream scale, and octopus flesh.…”

“…Pseudoalteromonas agarivorans NW4327, the primary pathogen of the disease affecting the Great Barrier Reef sponge Rhopaloeides odorabile, secretes two collagenases (both are metalloproteases) that degrade R. odorabile skeletal fibers (Bhattacharya et al 2018(Bhattacharya et al , 2019Choudhury et al 2015). One collagenase, that has a molecular mass of 116.25 kDa and requires Ca 2+ and Zn 2+ as cofactors, is a TonB-dependent receptor, having a carboxypeptidase regulatory-like domain in the N-terminus along with an outer membrane channel superfamily domain (Bhattacharya et al 2018). The other collagenase, a U32 metalloprotease, has a molecular mass of 52.51 kDa, contains two Ca 2+ , and has only 13% identity with known hydrolases.…”

“…SM9913 produces more than ten proteases (Yang et al 2019), three of which (the serine proteases MCP-01 and MCP-03 and the metalloprotease MCP-02), have been characterized (Gao et al 2010;Ran et al 2013;Yan et al 2009). Pseudoalteromonas agarivorans NW4327 contains 30 genes encoding proteases with a putative signal peptide; two of these (both collagenases) have been characterized (Bhattacharya et al 2018(Bhattacharya et al , 2019Choudhury et al 2015). The secreted Pseudoalteromonas proteases include a variety of serine proteases and metalloproteases of different families, and the substrate specificity of these proteases is diverse.…”

“…Pseudoalteromonas agarivorans NW4327 is a pathogen of the Great Barrier Reef Sponge Rhopaloeides odorabile. Two collagenases secreted by strain NW4327 can degrade R. odorabile skeletal fibers (Bhattacharya et al 2018(Bhattacharya et al , 2019. Pseudoalteromonas sp.…”

Genome sequence and transcriptomic profiles of a marine bacterium, Pseudoalteromonas agarivorans Hao 2018

“…Pseudoalteromonas produce a series of chemicals that are active against the target organisms, which may provide benefits for the bacteria regarding nutrient competition and colonization 8 . Pseudoalteromonas also can produce diverse extracellular enzymes that are of potential biotechnological interest, such as protease 16 , phospholipase 17 , agarose 18 , and collagenase 19 . Considering the potential applications and frequent occurrence of members of this genus, Pseudoalteromonas has attracted great interest from many researchers.…”