A new cell line from larval ovaries of Spodoptera Litura (F.) (Lepidoptera: Noctuidae)

Pant, Usha; Athawale, S S; Sudeep, A. B.; Banerjee, Kalyan

doi:10.1007/s11626-997-0136-9

Cited by 11 publications

(2 citation statements)

References 6 publications

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“…4c). This result implies that the cell line may be applied to the research of SpltNPV, for which a relative limited cell lines are able to be available (Jen and Lin 1995;Mitsuhashi 1995;Shih et al 1997;Pant et al 1998Pant et al , 2000Paul and Ramakrishnan 2000). Further work to clone the IOZCAS-Spex XI cell line should be performed to attempt to gener-…”

Section: Characterizationmentioning

confidence: 99%

A new cell line from Spodoptera exigua (Lepidoptera: Noctuidae) and its differentially expressed genes

Zhang

et al. 2011

J Applied Entomology

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A new cell line, designated IOZCAS‐Spex XI, was established from the pupal ovaries of Spodoptera exigua (Lepidoptera: Noctuidae) in TNM‐FH medium containing 10% foetal bovine serum. The spherical cells were predominant among the various cell types. The population‐doubling time during the logarithmic phase of growth was 81.7 h. It was confirmed that the cell line originated from S. exigua by DAF‐PCR technique. Analysis of susceptibility to baculovirus showed that the new cell line was susceptible to S. exigua nucleopolyhedrovirus (SeNPV), Autographa californica multiple NPV (AcMNPV) and slightly susceptible to S. litura NPV (SpltNPV), while not permissive to Helicoverpa armigera NPV and Hyphantria cunea NPV (HcNPV). Real‐Time PCR analysis was carried out to compare some differentially expressed genes between the cell line and the primary culture. The result showed that marked significant differences were observed in the expression of the genes of SUMO‐1 activating enzyme, BCCIP‐like protein, 10 kDa HSP, CypA, receptor for activated PKC, PDI‐like protein ERp57, ALDH, DEAD box ATP‐dependent RNA helicase‐like protein (P < 0.01), while a significant difference was obtained in the expression of GST gene between the cell line and the primary culture (P < 0.05).

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Section: Characterizationmentioning

confidence: 99%

A new cell line from Spodoptera exigua (Lepidoptera: Noctuidae) and its differentially expressed genes

Zhang

et al. 2011

J Applied Entomology

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“…The rRNA molecule has species-specific variable sequences that are flanked by highly conserved sequences. The cell line from larval and pupal ovaries were established from the tobacco cut worm (Spodoptera litura; Lepidoptera: Noctuidae) and termed as NIV-SU-992 and NIV-SU-893 respectively (9,10). The hypervariable amplified product can be used for obtaining sequence information by direct DNA sequencing, and its comparison can be used to identify, classify, and characterize the species.…”

Section: Dear Editormentioning

confidence: 99%