Available Lepidopteran Insect Cell Lines

Lynn, Dwight E.; Harrison, Robert L.

doi:10.1007/978-1-4939-3043-2_6

Cited by 19 publications

(4 citation statements)

References 90 publications

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“…Subsequently, other uses emerged for members of Baculoviridae owing to (i) the facilities offered by genetic engineering through viral-DNA manipulation (Luckow et al 1993), (ii) the natural condition that the viral genome is infective per se in host cells (Rohrmann 2019), and (iii) the availability of more than 320 insect cell lines capable of multiplying baculoviruses under in vitro conditions (Lynn and Harrison 2016). Unlike the circumstance in the agricultural sector, most of these new applications derive from a single alphabaculovirus, AcMNPV.…”

Section: Baculoviruses In Biotechnologymentioning

confidence: 99%

Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies

Targovnik

Simonin

Callum

et al. 2021

Appl Microbiol Biotechnol

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Baculoviruses are insect pathogens widely used as biotechnological tools in different fields of life sciences and technologies. The particular biology of these entities (biosafety viruses 1; large circular double-stranded DNA genomes, infective per se; generally of narrow host range on insect larvae; many of the latter being pests in agriculture) and the availability of molecular-biology procedures (e.g., genetic engineering to edit their genomes) and cellular resources (availability of cell lines that grow under in vitro culture conditions) have enabled the application of baculoviruses as active ingredients in pest control, as systems for the expression of recombinant proteins (Baculovirus Expression Vector Systems-BEVS) and as viral vectors for gene delivery in mammals or to display antigenic proteins (Baculoviruses applied on mammals-BacMam). Accordingly, BEVS and BacMam technologies have been introduced in academia because of their availability as commercial systems and ease of use and have also reached the human pharmaceutical industry, as incomparable tools in the development of biological products such as diagnostic kits, vaccines, protein therapies, and-though still in the conceptual stage involving animal models-gene therapies. Among all the baculovirus species, the Autographa californica multiple nucleopolyhedrovirus has been the most highly exploited in the above utilities for the human-biotechnology field. This review highlights the main achievements (in their different stages of development) of the use of BEVS and BacMam technologies for the generation of products for infectious and noninfectious human diseases. Key points• Baculoviruses can assist as biotechnological tools in human health problems.• Vaccines and diagnosis reagents produced in the baculovirus platform are described.• The use of recombinant baculovirus for gene therapy-based treatment is reviewed.

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Section: Baculoviruses In Biotechnologymentioning

confidence: 99%

Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies

Targovnik

Simonin

Callum

et al. 2021

Appl Microbiol Biotechnol

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“…At 24 h post transfection, the medium was replaced with fresh medium containing 10 μg/mL puromycin and incubated for 2-3 weeks with occasional replacements. Clones of puromycin-resistant cells were isolated using the terminal dilution method (Lynn and Harrison, 2016). Wells containing cells were examined by fluorescence microscopy using a Nikon eclipse ti with a GFP filter.…”

Section: Stably Cell Lines Expressing Gfp and Gfp::pepagmentioning

confidence: 99%

A simplified strategy to package foreign proteins into baculovirus occlusion bodies without engineering the viral genome

Fabre

Masson

Haase

et al. 2020

Journal of Biotechnology

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“…The silkworm ( Bombyx mori ) is a model lepidopteran insect with many available research tools, including genome information, transgenic methods, and cell lines 15 – 17 . In this study, we aimed to develop a practical JHSI by establishing a JH screening system for HTS using a B. mori cell line, in which the JH signaling pathway had been characterized 18 – 22 .…”

Section: Introductionmentioning

confidence: 99%

Identification of a juvenile-hormone signaling inhibitor via high-throughput screening of a chemical library

Kayukawa

Furuta

Nagamine

et al. 2020

Sci Rep

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Insecticide resistance has recently become a serious problem in the agricultural field. Development of insecticides with new mechanisms of action is essential to overcome this limitation. Juvenile hormone (JH) is an insect-specific hormone that plays key roles in maintaining the larval stage of insects. Hence, JH signaling pathway is considered a suitable target in the development of novel insecticides; however, only a few JH signaling inhibitors (JHSIs) have been reported, and no practical JHSIs have been developed. Here, we established a high-throughput screening (HTS) system for exploration of novel JHSIs using a Bombyx mori cell line (BmN_JF&AR cells) and carried out a large-scale screening in this cell line using a chemical library. The four-step HTS yielded 69 compounds as candidate JHSIs. Topical application of JHSI48 to B. mori larvae caused precocious metamorphosis. In ex vivo culture of the epidermis, JHSI48 suppressed the expression of the Krüppel homolog 1 gene, which is directly activated by JH-liganded receptor. Moreover, JHSI48 caused a parallel rightward shift in the JH response curve, suggesting that JHSI48 possesses a competitive antagonist-like activity. Thus, large-scale HTS using chemical libraries may have applications in development of future insecticides targeting the JH signaling pathway.

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Available Lepidopteran Insect Cell Lines

Cited by 19 publications

References 90 publications

Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies

Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies

A simplified strategy to package foreign proteins into baculovirus occlusion bodies without engineering the viral genome

Identification of a juvenile-hormone signaling inhibitor via high-throughput screening of a chemical library

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