A new Brucella canis species-specific PCR assay for the diagnosis of canine brucellosis

Kang, Sung-Il; Lee, Sang-Eun; Kim, Jiyeon; Lee, Kichan; Kim, Jongwan; Lee, Hyang-Keun; Sung, So-Ra; Heo, Young-Ran; Jung, Suk-Chan; Her, Moon

doi:10.1016/j.cimid.2014.07.003

Cited by 22 publications

(40 citation statements)

References 22 publications

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“…This assay has a high sensitivity for detecting DNA of B. canis . Therefore, BcSS‐PCR is a rapid, low‐cost, accurate, specific and sensitive assay for identification of B. canis strains that may be used to amplify DNA from clinical samples of dogs with brucellosis (Kang et al, ). Overall, 63 B. canis strains were widely distributed among 16 provinces of China, with the majority being distributed in the southern regions and Beijing.…”

Section: Discussionmentioning

confidence: 99%

“…Following collection of all of the strains, DNA was extracted with a Nucleic Acid Automatic Extraction System (LLXBIO China Ltd.) using a single loop of fresh bacterial cells that had been cultured for 48 hr on Brucella agar and inactivated by boiling. Subsequently, BcSS‐PCR was applied to confirm the results of conventional identification methods (Kang et al, ).…”

Section: Methodsmentioning

confidence: 99%

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Investigation of the molecular epizootiological characteristics and tracking of the geographical origins ofBrucella canisstrains in China

Liu

Wang

et al. 2019

Transbound Emerg Dis

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Brucellosis is a global pandemic infectious zoonosis. Brucella canis is a rare source of human brucellosis in China, and its public health significance remains under debate. Moreover, data pertaining to the epizootiological characteristics and geographical origin of B. canis on a nationwide scale are limited, and the risk to public safety posed by B. canis infections is unknown. The MLVA (multilocus variable‐number tandem repeat analysis) assay can be helpful to analyse epidemiological correlations among Brucella isolates and to track their geographic origins. To accomplish this task, MLVA‐16 was used to analyse the epidemiological links of 63 isolates obtained from dogs and humans. Sixty‐three B. canis strains were sorted into three large clusters (A, B and C) and 50 different genotypes (GT1–50), and 43 unique genotypes were represented by single isolates, suggesting that these strains had no obvious epidemiological links and that canine brucellosis is predominantly sporadic in China. The other seven shared genotypes (among a total of 20 isolates) were each represented by two to eight isolates, indicating that strains from each shared genotype were epidemiologically correlated. Five of the shared genotypes were from 16 strains obtained from Beijing, indicating that canine brucellosis in Beijing originates from multipoint outbreaks with multiple sources of infection. Based on comprehensive case analysis of clinical B. canis infection, we preliminarily suggest that human B. canis infections are associated with Mycoplasma pneumoniae infection that results in decreased patient immunity. B. canis may have limited epidemiological significance for the healthy population, but it remains a significant threat to the canine breeding industry and to humans who come into close contact with dogs. Based on MLVA‐11 data, B. canis strains were clustered into 16 genotypes and divided into five evolutionary branches; these data confirm that this population covers an extensive geographic area and exhibits characteristics of the origin and evolution of co‐existing introduced and locally native lineages. We believe this study will contribute to strengthening efforts to prevent and control canine brucellosis and to improve public understanding of the health risks posed by B. canis.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Investigation of the molecular epizootiological characteristics and tracking of the geographical origins ofBrucella canisstrains in China

Liu

Wang

et al. 2019

Transbound Emerg Dis

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“…Low concentration of bacteria may be excreted in saline, milk and nasal secretions. Uninfected dogs living with infected animals of the same sex were found to acquire the infection within 6 months (Wanke, 2004;Makloski, 2011;Kaden et al, 2014;Castillo et al, 2014;Kang et al, 2014). The Microplate Agglutination Test (MAT), Rapid Slide Agglutination Test (RSAT) and the Tube Agglutination Test (TAT) are often used to detect antibodies to B. canis in dogs (Thakur et al, 2003;Tuemmers et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Seroprevalance of Canine Brucellosis and Toxoplasmosis in Female and Male Dogs and Relationship to Various Factors as Parity, Abortion and Pyometra

Ergene

Çelebі

Küçükaslan

2017

IJAR

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The objective of this study was to determine the seroprevalance of canine brucellosis and toxoplasmosis in female and male dogs and also determine the realtionship to various factors as parity, abortion and pyometra. Brucella canis is a disease of the reproductive tract that may cause late abortion, infertility and fail of conception with optimum insemination time in females and infection of the sexual organs in males. Toxoplasma gondii is an important obligate intracellular protozoan parasite which can affect all warm-blooded mammals and humans which may cause fatal diseases with severe problems, such as abortion. As a result, in this study B. canis was determined in low seroprevalence in some cases on the island (North Cyprus), T. gondii was determined as an important contagious parasite. Also reproductive parameters like parity, spaying, cyclicity could be important too and it was presented that extended evaluation of these factors is needed with further studies.

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“…DNA was extracted from the isolate to amplify the Brucella -specific genes ( 16S rRNA , BCSP31 , and Omp2 ) [5]. PCR targeting Omp31 , IS711 , and BCSS confirmed this isolate as B. melitensis [678] (Table 1). Draft whole genome sequence analysis assembled by CLC bio CLC Genomics Workbench 7.5.1 (Waltham, MA, USA) revealed that the isolate was B. melitensis (Fig.…”

mentioning

confidence: 99%

“…We used multiple gene targets to detect the Brucella genus using a common PCR assay [5] and also used Omp31 , IS711 , and BCSS gene targets to distinguish B. melitensis from the other Brucella spp. [678] (Table 1). …”

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confidence: 99%

First Case of Human Brucellosis Caused byBrucella melitensisin Korea

et al. 2016

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A new Brucella canis species-specific PCR assay for the diagnosis of canine brucellosis

Cited by 22 publications

References 22 publications

Investigation of the molecular epizootiological characteristics and tracking of the geographical origins ofBrucella canisstrains in China

Investigation of the molecular epizootiological characteristics and tracking of the geographical origins ofBrucella canisstrains in China

Seroprevalance of Canine Brucellosis and Toxoplasmosis in Female and Male Dogs and Relationship to Various Factors as Parity, Abortion and Pyometra

First Case of Human Brucellosis Caused byBrucella melitensisin Korea

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