A near-infrared fluorescent probe for monitoring viscosity in living cells, zebrafish and mice

Zhan, Jingting; Chen, Geng; Hao, Xinya; Song, Wenhui; Lin, Weiying

doi:10.1039/d0nj05957a

Cited by 6 publications

(2 citation statements)

References 40 publications

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“…In order to explore if QR could detect the microviscosity changes, we used a well-known ionophore monensin that facilitates the transport of Na + and K + ions between intracellular and extracellular spaces causing viscosity enhancement by swelling of mitochondria through the disruption of ionic balance. [27][28][29] The living U-87 MG cells were treated with 10 mM monensin and incubated for 30 min, 1 h, and 4 h before staining with 5 mM QR (Fig. 3A-D, F-I).…”

Section: Mapping Intracellular Microviscositymentioning

confidence: 99%

Construing the metaxin-2 mediated simultaneous localization between mitochondria and nucleolus using molecular viscometry

et al. 2022

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Section: Mapping Intracellular Microviscositymentioning

confidence: 99%

Construing the metaxin-2 mediated simultaneous localization between mitochondria and nucleolus using molecular viscometry

et al. 2022

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“…In order to explore if QR could detect the microviscosity changes, we used a well-known ionophore monensin that facilitates the transport of Na + and K + ions between intracellular and extracellular spaces causing viscosity enhancement by swelling of mitochondria through the disruption of ionic balance. [44][45][46] The living U-87 MG cells were treated with 10 µM monensin and incubated for 30 min, 1 h, and 4 h before staining with 5 µM QR. (Figure 3A-D, F-I).…”

Section: Mapping Intracellular Microviscositymentioning

confidence: 99%

Construing the communication between powerhouse and ribosomal factory of cell: A small molecular viscometer comes to the rescue

Dutta

Das

Gupta

et al. 2022

Preprint

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Fluorescent probes for specific inter-organelle communication are of massive significance as such communication is essential for a diverse range of cellular events. Here, we present the microviscosity-sensitive fluorescence marker, Quinaldine red (QR), and its dual organelle targeting light-up response in live cells. This biocompatible probe was able to localize in mitochondria and nucleolus simultaneously. While QR was able to sense the viscosity change inside these compartments under the induced effect of an ionophore and ROS-rich microenvironment, the probe’s ability to stain mitochondria remained unperturbed even after protonophore-induced depolarization. Consequently, a systematic quantification was performed to understand the alteration of microviscosity. Similar behavior in two distinct organelles implied that QR binds to Metaxin-2 protein, common to mitochondrial and nucleolar proteomes. We believe this is the first of its kind investigation that identifies the inter-organelle communications marker and opens up a new dimension in this field.

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Hemicyanine‐Based Near‐Infrared Activatable Probes for Imaging and Diagnosis of Diseases

et al. 2021

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Molecular activatable probes with near‐infrared (NIR) fluorescence play a critical role in in vivo imaging of biomarkers for drug screening and disease diagnosis. With structural diversity and high fluorescence quantum yields, hemicyanine dyes have emerged as a versatile scaffold for the construction of activatable optical probes. This Review presents a survey of hemicyanine‐based NIR activatable probes (HNAPs) for in vivo imaging and early diagnosis of diseases. The molecular design principles of HNAPs towards activatable optical signaling against various biomarkers are discussed with a focus on their broad applications in the detection of diseases including inflammation, acute organ failure, skin diseases, intestinal diseases, and cancer. This progress not only proves the unique value of HNAPs in preclinical research but also highlights their high translational potential in clinical diagnosis.

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A near-infrared fluorescent probe for monitoring viscosity in living cells, zebrafish and mice

Abstract: ZM-V was designed by the interior imidazole and benzopyrene moieties serving as rotators, which could spin around multiple C-C bonds in the conjugated skeleton. The fluorescence intensity of ZM-V changed...

Cited by 6 publications

References 40 publications

Construing the metaxin-2 mediated simultaneous localization between mitochondria and nucleolus using molecular viscometry

Construing the metaxin-2 mediated simultaneous localization between mitochondria and nucleolus using molecular viscometry

Construing the communication between powerhouse and ribosomal factory of cell: A small molecular viscometer comes to the rescue

Hemicyanine‐Based Near‐Infrared Activatable Probes for Imaging and Diagnosis of Diseases

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