Construing the metaxin-2 mediated simultaneous localization between mitochondria and nucleolus using molecular viscometry

Abstract

Fluorescent probes for specific inter-organelle communication are of massive significance as such communication is essential for a diverse range of cellular events. Here, we present the microviscosity-sensitive fluorescence marker, Quinaldine...

Tracking endoplasmic reticulum viscosity during ferroptosis and autophagy using a molecular rotor probe

“…[11][12][13][14][15] The viscosity alteration in organelles such as the lysosome and mitochondria is widely reported. [16][17][18][19][20][21] However, there are very few reports accounting for the ER viscosity changes, and analyzing ER viscosity fluctuation during different pathological conditions is barely explored. [22][23][24] Herein, we have developed and synthesized a viscosity sensitive molecular rotor to monitor the ER fate during different biological cell death modes.…”

Dual role far red fluorescent molecular rotor for decoding the plasma membrane and mitochondrial viscosity

“…In recent years, groundbreaking research on the contact sites of the different organelles has demonstrated their emerging role in regulating cellular function. [33][34][35] Recent reports have shown the interaction of mitochondria-lysosome, mitochondria-endoplasmic reticulum, and mitochondria-nucleoli utilizing small molecular fluorescent probes illustrated in Scheme 1. [35][36][37][38][39][40][41] In most of these reports, simultaneous dual organelle labeling is demonstrated using different organelle-targeting moieties.…”

“…[33][34][35] Recent reports have shown the interaction of mitochondria-lysosome, mitochondria-endoplasmic reticulum, and mitochondria-nucleoli utilizing small molecular fluorescent probes illustrated in Scheme 1. [35][36][37][38][39][40][41] In most of these reports, simultaneous dual organelle labeling is demonstrated using different organelle-targeting moieties. Solute-mediated chemical interactions between sub-plasmalemmal mitochondria and plasma membrane are known to regulate the mitochondria and plasma membrane functions.…”

Triphenylamine-equipped 1,8-naphthaolactam: a versatile scaffold for the custom design of efficient subcellular imaging agents

“…In contrast, fluorescence imaging is extensively applied in basic biomedical research due to its high sensitivity, simple operation, and real-time models, along with observational objects ranging from micrometer-scale organelles and cells to large-size tissue. [13][14][15][16][17][18] As the source of emission signals, fluorescence bioprobes are of great importance in this imaging system and very recently have made outstanding contributions to various innovative bioimaging applications. [19][20][21][22][23][24] Given the complex and dynamic features of biological systems, an ideal fluorescent probe in a bioimaging system is demanded to meet the following requirements: (1) bright and constant emission, (2) significant Stokes shifts, (3) excellent biocompatibility and photostability, and (4) easy functional modifiability.…”

“…In contrast, fluorescence imaging is extensively applied in basic biomedical research due to its high sensitivity, simple operation, and real-time models, along with observational objects ranging from micrometer-scale organelles and cells to large-size tissue. 13–18 As the source of emission signals, fluorescence bioprobes are of great importance in this imaging system and very recently have made outstanding contributions to various innovative bioimaging applications. 19–24…”