A Nanobiosensor Based on 4-Hydroxyphenylpyruvate Dioxygenase Enzyme for Mesotrione Detection

García, Pamela Soto; Moreau, A. L. D.; Ierich, Jéssica Cristiane Magalhães; Vig, Ana Carolina Araujo; Higa, Akemi Martins; Oliveira, Guedmiller Souza de; Abdalla, Fábio Camargo; Hausen, Moema de Alencar; Leite, Fábio L.

doi:10.1109/jsen.2014.2371773

Cited by 25 publications

(15 citation statements)

References 37 publications

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“…The cantilever surface was coated with an APTES layer, as previously described by Garcia et al 8 All cantilevers were¯rst cleaned under UV light and then treated with ozone in a ProCleaner (UV. PC.220, Bioforce Nanosciences, Salt Lake City, UT) for 20 min.…”

Section: Cantilever Functionalizationmentioning

confidence: 99%

“…4 The atomic force microscopy (AFM) cantilever presents a smart platform for the development of highly sensitive and selective sensors that can detect assorted physical, chemical, and biological events. [5][6][7] Its means of detection are based on two AFM techniques: (1) spectroscopy of AFM tips through force curve measurements, 8 and (2) measurement of cantilever de°ection, which analyzes surface stress due to the chemical bindings or reactions occurring on immobilized surfaces. 5,9 Other approaches to de°ection analysis can be accomplished by changing the resonance frequency of the cantilever to the dynamic mode.…”

Section: Introductionmentioning

confidence: 99%

“…5,9 Other approaches to de°ection analysis can be accomplished by changing the resonance frequency of the cantilever to the dynamic mode. 5,9 Both devices (tip or cantilever) can be used in either the liquid or gaseous environment to detect proteins, 5,10,11 DNA, 12,13 RNA, 14 or even pesticides, 8 in real-time and with high sensitivity. Nanomechanical sensors based on cantilever devices can be applied to target molecules for medical purposes, 5 such as in the detection of neurological diseases.…”

Section: Introductionmentioning

confidence: 99%

“…19 Recent studies have demonstrated the interaction of AFM tips functionalized with molecules of environmental interest, such as 4-hydroxyphenylpyruvate dioxygenase (HPPD) and the herbicide mesotrione; these studies showed high e±ciency of detection based on spectroscopy of force curves. 8 Enzymes are molecules that are directly related to metabolism and are responsible for essential biochemical and genetic processes in all organisms. Their actions can be blocked through enzymatic inhibitors that disrupt the molecule's functions; in plants, such enzymatic inhibitors can cause death.…”

Section: Introductionmentioning

confidence: 99%

“…20,21 When this enzyme is de¯cient in humans, the resulting low rate of tyrosine catabolism can cause severe metabolic disorders such as tyrosinemia or hawkinsinuria. 8 Likewise, in plants, the inhibition of HPPD results in low levels of chlorophyll and carotenoids, lead to the whitening of leaves and eventually to plant death. 21 In the last 50 years, with the goal of improving high yields at harvest time, the use of agrochemicals has increased dramatically.…”

Section: Introductionmentioning

confidence: 99%

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Nanomechanical Cantilever-Based Sensor: An Efficient Tool to Measure the Binding Between the Herbicide Mesotrione and 4-Hydroxyphenylpyruvate Dioxygenase

Rodrigues

Ierich

Andrade

et al. 2017

NANO

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Nanomechanical biosensors based on atomic force microscopy (AFM) cantilevers have garnered considerable attention. AFM cantilevers are devices that can detect a target either via a surface functionalization process based on immobilization through molecular adsorption, or through the selective chemical binding of a speci¯c molecule, transforming the device into a speci¯c biosensor. In this study, we demonstrate that functionalized AFM cantilevers could be used, in a process involving self-assembling layers, to create a homogeneous surface layer of the widely used herbicide mesotrione. Controlled experiments to evaluate its detection were performed, and binding between mesotrione and its target molecule, 4-hydroxyphenylpyruvate dioxygenase (HPPD), was evaluated using de°ection curves of functionalized cantilevers interacting with mesotrione. The cantilevers worked as nanomechanical sensors inside a°uid cell device, under di®erent ¶ Corresponding author. 1750079-1NANO: Brief Reports and Reviews Vol. 12, No. 7 (2017) concentrations of HPPD diluted in PBS. After evaluating increasing concentrations of HPPD, the de°ection curves showed a clear, dose-dependent pattern. The homogeneous dispersion of mesotrione on the cantilevers was assessed by confocal microscopy, and this corroborated the functionalization method. Thus, the results obtained by this functionalized cantilever presented a high e±ciency in detecting binding between HPPD and mesotrione molecules at concentrations as low as 17 ng mL À1 . In this way, as a preliminary step for a future environmental contaminants nanosensor development, the described detection method showed a suitable capability for molecular recognition at the nanoscale.

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Section: Cantilever Functionalizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nanomechanical Cantilever-Based Sensor: An Efficient Tool to Measure the Binding Between the Herbicide Mesotrione and 4-Hydroxyphenylpyruvate Dioxygenase

Rodrigues

Ierich

Andrade

et al. 2017

NANO

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In vivo fluorescent screening for HPPD‐targeted herbicide discovery

Zhang

Guo

et al. 2022

Pest Management Science

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Background 4‐Hydroxyphenylpyruvate dioxygenase (HPPD), playing a critical role in vitamin E and plastoquinone biosynthesis in plants, has been recognized as one of the most important targets for herbicide discovery for over 30 years. Structure‐based rational design of HPPD inhibitors has received more and more research interest. However, a critical challenge in the discovery of new HPPD inhibitors is the common inconsistency between molecular‐level HPPD‐based bioevaluation and the weed control efficiency in fields, due to the unpredictable biological processes of absorption, distribution, metabolism, and excretion. Results In this study, we developed a fluorescent‐sensing platform of efficient in vivo screening for HPPD‐targeted herbicide discovery. The refined sensor has good capability of in situ real‐time fluorescence imaging of HPPD in living cells and zebrafish. More importantly, it enabled the direct visible monitoring of HPPD inhibition in plants in a real‐time manner. Conclusion We developed a highly efficient in vivo fluorescent screening method for HPPD‐targeted herbicide discovery. This discovery not only offers a promising tool to advance HPPD‐targeted herbicide discovery, but it also demonstrates a general path to develop the highly efficient, target‐based, in vivo screening for pesticide discovery. © 2022 Society of Chemical Industry.

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