A nano-sized chitosan particle based electrochemical aptasensor for sensitive detection ofP. aeruginosa

Abstract

In this research, a glassy carbon electrode (GCE) was modified with nano-sized chitosan particles (NCs) by the drop-casting method for ultrasensitive detection of Pseudomonas aeruginosa (P. aeruginosa).

Pathogen detection with electrochemical biosensors: Advantages, challenges and future perspectives

“…An electrochemical aptasensor was developed for the first time for the detection of Pseudomonas aeruginosa from blood serum specimens with EIS [ 50 ]. For GCE modification, nano-sized chitosan particles (NCs) were prepared, and the modified GCEs were treated with glutaraldehyde and plunged into aptamer solution.…”

Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa

“…Impedimetric biosensors have drawn much interest in recent years, owing to their reduced matrix interference, relatively simple electrical measurements, and the possibility of automation (Paniel et al, 2013). Roushani et al (2019) constructed an impedimetric aptasensor using a glassy carbon electrode (GCE) modified with silver nanoparticles (AgNPs) ( Figure 7A). Free amino groups of the aptamer were covalently coupled to the AgNP/GCE surface.…”

“…(C) Schematic illustration of aptasensor fabrication (a) and "signal-off" detection of P. aeruginosa (b) (Shahrokhian and Ranjbar, 2019). FIGURE 7 | (A) Schematic view of the development of a P. aeruginosa/aptamer/silver nanoparticle/glassy carbon electrode complex for P. aeruginosa detection (Roushani et al, 2019). (B) Schematic view of the development of a P. aeruginosa/aptamer/glutaraldehyde (Glu)/nanosized chitosan (NC)/glassy carbon electrode complex for P. aeruginosa detection (Sarabaegi and Roushani, 2019).…”

“…This assay was used to measure P. aeruginosa in spiked serum samples, with a lower LOD of 33 CFU/mL. Sarabaegi and Roushani (2019) designed an electrochemical aptasensor ( Figure 7B ) with a specific aptamer covalently linked to a GCE surface, and the hexacyanoferrate redox system as an electrochemical probe. They used nanosized chitosan (NC) particles to modify the GCE, form a sensitive layer, and improve performance.…”

Recent Advances in Aptamer Sensors

“…Several nanomaterials have been synthesized with different sizes and shapes such as gold nanoparticles (AuNPs), silica nanoparticles, silver nanoparticles (AgNPs), magnetic nanoparticles (MNPs), and carbon quantum dots (CQDs) as unique templates for many applications such as biomaterial assays; the diagnosis, monitoring, and treatment of disease; and drug delivery [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. Interestingly, nanomaterials are being used as potential candidates for immobilization using aptamers to obtain nanosensor probes for several targets, with more amplification and various signals such as colorimetric, fluorometric, electrochemical, or optical [ 31 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ]. Sensor fabrication mainly depends on target recognition and signal transduction.…”