Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa

Abstract

Increasing concerns about nosocomial infection, food and environmental safety have prompted the development of rapid, accurate, specific and ultrasensitive methods for the early detection of critical pathogens. Pseudomonas aeruginosa is one of the most common pathogens that cause infection. It is ubiquitous in nature, being found in water, soil, and food, and poses a great threat to public health. The conventional detection technologies are either time consuming or readily produce false positive/negative results, which makes them unsuitable for early diagnosis and spot detection of P. aeruginosa. To circumvent these drawbacks, many efforts have been made to develop biosensors using aptamers as bio-recognition elements. Various aptamer-based biosensors for clinical diagnostics, food, and environmental monitoring of P. aeruginosa have been developed in recent years. In this review, we focus on the latest advances in aptamer-based biosensors for detection of P. aeruginosa. Representative biosensors are outlined according to their sensing mechanisms, which include optical, electrochemical and other signal transduction methods. Possible future trends in aptamer biosensors for pathogen detection are also outlined.

Recent Advances in Electrochemical Biosensors for the Detection of Salmonellosis: Current Prospective and Challenges

“…The identification of aptamers, silver signal amplification, nanogold tagging, and a quick, specific, and visible detection approach for Salmonella typhimurium was developed in a detection range of 10–10 6 CFU/mL and LOD of 7 CFU/mL for Salmonella typhimurium with high specificity and affinity ( Figure 2 ) [ 22 ]. They have evolved into a potent target recognition tool with unrivalled intrinsic benefits such as chemical and physical stability, simplicity of synthetization and modification, non-toxicity, structural memory, and enhanced half-life [ 23 ]. When designing several biosensing apparatuses for the detection of Salmonella , aptamers have been employed as an alternative to traditional antibodies.…”

Recent Advancements in Nanosystem-Based Molecular Beacons for RNA Detection and Imaging

“…The essence of aptamers is a unique three-dimensional structure folded from a single-stranded nucleic acid molecule, and they have a great variety of forms such as hairpin, internal loops, pseudoknot, bulge, and G-tetramer (G-tetramer), etc. They can be used to modify various functional groups or materials . Due to its high affinity to the target, aptamers are also called “chemical antibodies”.…”

“…They can be used to modify various functional groups or materials. 72 Due to its high affinity to the target, aptamers are also called "chemical antibodies". Compared with protein antibodies, aptamers have high affinity, simple synthesis, low molecular weight, high stability of the chemical structure, fewer adverse reactions, and low immunogenicity.…”

Aptamers: A prospective tool for infectious diseases diagnosis

“…Pseudomonas aeruginosa ( P. aeruginosa ) is another vital opportunistic pathogen that can induce hospital‐acquired infection, featured by easy colonization, easy mutation and multi‐drug resistance, and it can elicit lung infection, bloodstream infection (BSI) and bacteria sepsis. With approximately 49 million septic patients and 11 million mortalities across the globe, the early diagnosis and on‐site detection of P. aeruginosa can reduce the incidence and death rate due to P. aeruginosa‐ induced BSI 72,73 . In 2017, Jennifer used the CELL‐SELEX method to obtain DNA aptamers specific for live P. aeruginosa and the result demonstrated that the aptamer exhibited a remarkable discrimination ability in the face of P. aeruginosa 74 .…”