A Mutation in the eln2 Gene Encoding a Cytochrome P450 of Coprinus cinereus Affects Mushroom Morphogenesis

Muraguchi, Hajime; Kamada, Takashi

doi:10.1006/fgbi.2000.1184

Cited by 73 publications

(42 citation statements)

References 32 publications

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“…6B). It is noteworthy to mention that P450s found in C. versicolor show less than 40% identity with previously reported P450s isolated from other basidiomycetes, CYP63-1A from P. chrysosporium (Kullman and Matsumura 1997) and CYP502 from Coprinus cinerius (Muraguchi and Kamada 2000). These results might be indicative of P450 molecular species of basidiomycetes being developed differently in each fungal species.…”

Section: Differential Display Rt-pcr Analysismentioning

confidence: 53%

Identification and characterization of novel cytochrome P450 genes from the white-rot basidiomycete, Coriolus versicolor

Ichinose¹

2002

Applied Microbiology and Biotechnology

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Using a reverse-transcription polymerase chain reaction (RT-PCR) technique, cytochrome P450 genes were cloned from the lignin-degrading basidiomycete, Coriolus versicolor. One possible P450 gene was identified, which consisted of 1,672 nucleotides and a poly(A) tail and encoded a deduced protein containing 449 amino acids. The deduced amino acid sequence revealed the presence of the P450 heme-binding motif, strongly suggesting that this protein belongs to the P450 superfamily, then designated CYP512A1. The deduced protein showed sequential similarity to other known P450s from several micro-organisms, such as Aspergillus terreus, Gibberella fujikuroi, and Neurospora crassa, with 30-35% identity. Since the identity of the amino id sequence was less than 40% with any other P450s, this protein was suggested to be the first member of a new family of cytochrome P450. In addition, a differential display RT-PCR analysis showed the expression of the other P450 genes, which were up-regulated by the addition of dibenzothiophene and 4-methyldibenzothiophene-5-oxide. Using the 5' rapid amplification of cDNA ends method, a 520-nucleotide sequence, including the P450 motif-coding region, was determined for one clone. The deduced protein showed high similarity to CYP512A1 but less than 40% identity with P450s from other organisms. A chemical stress-responsive expression of P450 is suggested for the first time in basidiomycetes.

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Section: Differential Display Rt-pcr Analysismentioning

confidence: 53%

Identification and characterization of novel cytochrome P450 genes from the white-rot basidiomycete, Coriolus versicolor

Ichinose¹

2002

Applied Microbiology and Biotechnology

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“…It was known that a mutation in the cytochrome P450 gene was the cause of abnormal stipe elongation of C. cinereus (Muraguchi and Kamada, 2000) and in A. bisporus, the cypA gene encoding cytochrome P450 was specifically expressed in the fruit body (De Groot et al, 1997). However, Northern blot analysis in C. cinereus and our EST analysis results showed that the gene product occurred in both mycelia and fruit body cDNA library, although the sequence occurred only one time in mycelial ESTs and four times in the fruiting body.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Sequences Expressed during the Liquid-Cultured Mycelia and Fruit Body Stages of Pleurotus ostreatus

Lee

Kim

et al. 2002

Fungal Genetics and Biology

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“…(53) The eln2 gene was identified by a dominant mutation that affects pattern formation during the development of the fruit-body primordium, causing a dumpy primordium, which culminate in a mature fruit body with a short stipe (Fig. 3E).…”

Section: Mushroom Morphogenesismentioning

confidence: 98%

Molecular genetics of sexual development in the mushroom Coprinus cinereus

Kamada

2002

BioEssays

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101

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Sexual development in the mushroom Coprinus cinereus is under the control of two mating type loci, A and B. When two haploid homokaryons with compatible alleles at both A and B loci are mated, the coordinated activities of A- and B-regulated pathways lead to formation of a mycelium termed the dikaryon, in which the two nuclei from the mating partners pair in each cell without fusing. The dikaryon is a prolonged mycelial stage that can be induced to develop a multicellular structure, the mushroom, under proper environmental conditions. The two nuclei fuse in specialized cells on the mushroom and immediately undergo meiosis to complete the sexual life cycle. It has been established recently that the A genes encode two classes of homeodomain proteins while the B genes encode pheromones and their receptors. More recently, molecular genetics has been used to reveal genes that work downstream of the mating type genes to regulate dikaryon formation, mushroom morphogenesis, and meiosis.

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A Mutation in the eln2 Gene Encoding a Cytochrome P450 of Coprinus cinereus Affects Mushroom Morphogenesis

Cited by 73 publications

References 32 publications

Identification and characterization of novel cytochrome P450 genes from the white-rot basidiomycete, Coriolus versicolor

Identification and characterization of novel cytochrome P450 genes from the white-rot basidiomycete, Coriolus versicolor

Comparative Analysis of Sequences Expressed during the Liquid-Cultured Mycelia and Fruit Body Stages of Pleurotus ostreatus

Molecular genetics of sexual development in the mushroom Coprinus cinereus

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