Molecular genetics of sexual development in the mushroom <i>Coprinus cinereus</i>

Kamada, Takashi

doi:10.1002/bies.10083

Cited by 101 publications

(18 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The NanoLuc system, in contrast, performed well in our system. NanoLuc will be useful in analyzing cis -elements in the many genes that have been cloned in the long history of studying the genetics of mushroom-forming basidiomycetes 27, 28 . For instance, cis - analyses of PriA 29 , a marker gene for early development of the fruiting body, whose UTR elements has been analyzed 30 , will be informative in helping to unravel some aspects of the mechanism of the onset of fruiting body development.…”

Section: Discussionmentioning

confidence: 99%

Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system

Sugano

Suzuki

Shimokita

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Mushroom-forming basidiomycetes produce a wide range of metabolites and have great value not only as food but also as an important global natural resource. Here, we demonstrate CRISPR/Cas9-based genome editing in the model species Coprinopsis cinerea. Using a high-throughput reporter assay with cryopreserved protoplasts, we identified a novel promoter, CcDED1 pro, with seven times stronger activity in this assay than the conventional promoter GPD2. To develop highly efficient genome editing using CRISPR/Cas9 in C. cinerea, we used the CcDED1 pro to express Cas9 and a U6-snRNA promoter from C. cinerea to express gRNA. Finally, CRISPR/Cas9-mediated GFP mutagenesis was performed in a stable GFP expression line. Individual genome-edited lines were isolated, and loss of GFP function was detected in hyphae and fruiting body primordia. This novel method of high-throughput CRISPR/Cas9-based genome editing using cryopreserved protoplasts should be a powerful tool in the study of edible mushrooms.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system

Sugano

Suzuki

Shimokita

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In C. cinerea and other Agaricomycetes, each encounter of two monokaryotic hyphae results in promiscuous cell-cell fusion irrespective of whether or not they are compatible at MAT (18, 89). However, for the development and maintenance of the dikaryon, nuclei of interacting partners must harbor different allelic versions of genes in at least one sublocus of both P/R and HD loci (18, 89–91).…”

Section: Breeding Systems In the Basidiomycotamentioning

confidence: 99%

“…However, for the development and maintenance of the dikaryon, nuclei of interacting partners must harbor different allelic versions of genes in at least one sublocus of both P/R and HD loci (18, 89–91). Classical genetic analyses of these semi-compatible interactions in C. cinerea and S. commune have shown that the P/R system is involved in events that follow hyphal fusion, namely in the reciprocal exchange and migration of nuclei and clamp cell fusion (Fig.…”

Section: Breeding Systems In the Basidiomycotamentioning

confidence: 99%

Fungal Sex: The Basidiomycota

et al. 2017

View full text Add to dashboard Cite

SUMMARY Fungi of the Basidiomycota, representing major pathogen lineages and mushroom-forming species, exhibit diverse means to achieve sexual reproduction, with particularly varied mechanisms to determine compatibilities of haploid mating partners. For species that require mating between distinct genotypes, discrimination is usually based on both the reciprocal exchange of diffusible mating pheromones, rather than sexes, and the interactions of homeodomain protein signals after cell fusion. Both compatibility factors must be heterozygous in the product of mating, and genetic linkage relationships of the mating pheromone/receptor and homeodomain genes largely determine the complex patterns of mating-type variation. Independent segregation of the two compatibility factors can create four haploid mating genotypes from meiosis, referred to as tetrapolarity. This condition is thought to be ancestral to the basidiomycetes. Alternatively, co-segregation by linkage of the two mating factors, or in some cases the absence of the pheromone-based discrimination, yields only two mating types from meiosis, referred to as bipolarity. Several species are now known to have large and highly rearranged chromosomal regions linked to mating-type genes. At the population level, polymorphism of the mating-type genes is an exceptional aspect of some basidiomycete fungi, where selection under outcrossing for rare, intercompatible allelic variants is thought to be responsible for mating types that may number several thousand. Advances in genome sequencing and assembly are yielding new insights by comparative approaches among and within basidiomycete species, with the promise to resolve the evolutionary origins and dynamics of mating compatibility genetics in this major eukaryotic lineage.

show abstract

“…This system is only found in basidiomycetes because, unlike all other fungi studied so far, they possess two independent molecular determinants of mating type [17]. Sexual compatibility is usually determined at a first level by lipopetide pheromones and plasma membrane pheromone receptors that mediate cell-cell recognition, with the exception of some homobasidiomycetes (the lineage that includes the mushrooms) where fusion of homokaryotic hyphae is not restrained by pheromone-mediated interactions [2], [18]. After cell fusion, progression through the sexual cycle requires overcoming a second compatibility hurdle that relies on a heterodimeric homeodomain transcription factor (HD1/HD2), encoded by a pair of divergently transcribed, closely linked genes [3], [19]–[21].…”

Section: Introductionmentioning

confidence: 99%

A Deviation from the Bipolar-Tetrapolar Mating Paradigm in an Early Diverged Basidiomycete

2010

View full text Add to dashboard Cite

In fungi, sexual identity is determined by specialized genomic regions called MAT loci which are the equivalent to sex chromosomes in some animals and plants. Usually, only two sexes or mating types exist, which are determined by two alternate sets of genes (or alleles) at the MAT locus (bipolar system). However, in the phylum Basidiomycota, a unique tetrapolar system emerged in which four different mating types are generated per meiosis. This occurs because two functionally distinct molecular recognition systems, each encoded by one MAT region, constrain the selection of sexual partners. Heterozygosity at both MAT regions is a pre-requisite for mating in both bipolar and tetrapolar basidiomycetes. Tetrapolar mating behaviour results from the absence of genetic linkage between the two regions bringing forth up to thousands of mating types. The subphylum Pucciniomycotina, an early diverged lineage of basidiomycetes encompassing important plant pathogens such as the rusts and saprobes like Rhodosporidium and Sporidiobolus, has been so far poorly explored concerning the content and organization of MAT loci. Here we show that the red yeast Sporidiobolus salmonicolor has a mating system unlike any previously described because occasional disruptions of the genetic cohesion of the bipolar MAT locus originate new mating types. We confirmed that mating is normally bipolar and that heterozygosity at both MAT regions is required for mating. However, a laboratory cross showed that meiotic recombination may occur within the bipolar MAT locus, explaining tetrapolar features like increased allele number and evolution rates of some MAT genes. This pseudo-bipolar system deviates from the classical bipolar–tetrapolar paradigm and, to our knowledge, has never been observed before. We propose a model for MAT evolution in the Basidiomycota in which the pseudo-bipolar system may represent a hitherto unforeseen gradual form of transition from an ancestral tetrapolar system to bipolarity.

show abstract

Molecular genetics of sexual development in the mushroom Coprinus cinereus

Cited by 101 publications

References 90 publications

Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system

Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system

Fungal Sex: The Basidiomycota

A Deviation from the Bipolar-Tetrapolar Mating Paradigm in an Early Diverged Basidiomycete

Contact Info

Product

Resources

About