Comparative Analysis of Sequences Expressed during the Liquid-Cultured Mycelia and Fruit Body Stages of Pleurotus ostreatus

Lee, Seung-Ho; Kim, Beom-Gi; Kim, Kyungjin; Lee, Jin Sung; Yun, Doh-Won; Hahn, Jang-Ho; Kim, Gyu-Hyun; Lee, Kang-Hyo; Suh, Dong-Sang; Kwon, Suk‐Tae; Lee, Chang‐Soo; Yoo, Young-Bok

doi:10.1006/fgbi.2001.1310

Cited by 59 publications

(52 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The other 21% encoded protein sequences with higher similarity to other organisms, including bacteria, viruses, insects and other invertebrates. These percentages are similar to results obtained by other research groups studying filamentous fungi (Lee et al, 2002a;Trail et al, 2003). Twenty-nine of the 40 unisequences presented in Table 1 are novel P. pachyhrizi sequences.…”

Section: Resultssupporting

confidence: 89%

Soybean Rust: Five Years of Research

Tremblay¹

2011

Soybean - Molecular Aspects of Breeding

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 89%

Soybean Rust: Five Years of Research

Tremblay¹

2011

Soybean - Molecular Aspects of Breeding

View full text Add to dashboard Cite

“…The remaining TDFs (70%) did not have significant matches within the GenBank databases and may represent undescribed fungal genes, although this could also be partly due to the fragments being derived from either the 5′ or the 3′ untranslated messenger RNA (mRNA) regions. This relatively high proportion of TDFs showing no homology to GenBank entries is compatible with the range of 50-65% reported for expressed sequence tags (ESTs) in other filamentous fungi (Lee et al 2002;Ospina-Giraldo et al 2000;Skinner et al 2001) and might reflect the low number of database entries describing sequences of fungal origin.…”

Section: Discussionsupporting

confidence: 80%

Gene expression profiling of a Zn-tolerant and a Zn-sensitive Suillus luteus isolate exposed to increased external zinc concentrations

et al. 2007

View full text Add to dashboard Cite

Complementary DNA (cDNA)-amplified fragmentlength polymorphism (AFLP) was applied to analyze transcript profiles of a Zn-tolerant and a Zn-sensitive isolate of the ectomycorrhizal basidiomycete Suillus luteus, both cultured with and without increased external zinc concentrations. From the obtained transcript profiles that covered approximately 2% of the total expected complement of genes in S. luteus, 144 nonredundant, differentially expressed transcript-derived fragments (TDFs), falling in different classes of expression pattern, were isolated and sequenced. Thirty-six of the represented genes showed homology to function-known genes, whereas 6 matched unknown protein coding sequences, and 102 were possibly novel. Although relatively few TDFs were found to be responsive to the different zinc treatments, their modulated expression levels may suggest a different transcriptional response to zinc treatments in both isolates. Among the identified genes that could be related to heavymetal detoxification or the tolerance trait were genes encoding for homologues of a heat-shock protein, a putative metal transporter, a hydrophobin, and several proteins involved in ubiquitin-dependent proteolysis.

show abstract

“…An EST database containing more that 2,000 clones was recently set up for the widely cultivated edible mushroom Pleurotus ostreatus with the aim of providing information on differential gene expression during the transition of vegetative mycelium to spore-bearing structures (17). In other ascomycetes and basidiomycetes, the transition has already been demonstrated to be coupled to transcriptional changes in many genes which regulate reproductive development after induction (1,8,10,34,41,44).…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Differentially Expressed Genes in the Mycelium and Fruit Body of Tuber borchii

Lacourt¹,

Duplessis

Abbà³

et al. 2002

Appl Environ Microbiol

View full text Add to dashboard Cite

The transition from vegetative mycelium to fruit body in truffles requires differentiation processes which lead to edible fruit bodies (ascomata) consisting of different cell and tissue types. The identification of genes differentially expressed during these developmental processes can contribute greatly to a better understanding of truffle morphogenesis. A cDNA library was constructed from vegetative mycelium RNAs of the white truffle Tuber borchii, and 214 cDNAs were sequenced. Up to 58% of the expressed sequence tags corresponded to known genes. The majority of the identified sequences represented housekeeping proteins, i.e., proteins involved in gene or protein expression, cell wall formation, primary and secondary metabolism, and signaling pathways. We screened 171 arrayed cDNAs by using cDNA probes constructed from mRNAs of vegetative mycelium and ascomata to identify fruit body-regulated genes. Comparisons of signals from vegetative mycelium and fruit bodies bearing 15 or 70% mature spores revealed significant differences in the expression levels for up to 33% of the investigated genes. The expression levels for six highly regulated genes were confirmed by RNA blot analyses. The expression of glutamine synthetase, 5-aminolevulinic acid synthetase, isocitrate lyase, thioredoxin, glucan 1,3-␤-glucosidase, and UDP-glucose:sterol glucosyl transferase was highly up-regulated, suggesting that amino acid biosynthesis, the glyoxylate cycle pathway, and cell wall synthesis are strikingly altered during morphogenesis.Several truffle species are harvested all over the world in significant quantities, as the organoleptic properties (i.e., taste and flavor) of their edible ascomata are highly appreciated. The fruiting of ectomycorrhizal Tuber depends on a complex set of variables, including metabolites and signals produced by the host plant, the nutritional status of the substrate, and unknown environmental cues (e.g., humidity and temperature). The different types of cells and tissues of fruit bodies of ascomycetes (ascomata) are the result of a differentiation process leading to the production of asci containing meiospores (30). The molecular bases of such events are largely unknown, with the exception of those in some model fungi, such as Aspergillus nidulans (1) and Neurospora crassa (26), in which an interactive cascade of developmentally regulated genes regulates sporulation.Morphological descriptions of ascoma development in truffles are scarce and illustrate only advanced developmental stages (27). This situation is due to the hypogeous habitat of truffles, which leads to erratic sampling. In addition, symbiotic relationships are required for the development of the truffle fruit body (36), and fruit bodies cannot be produced in vitro. These features have hampered systematic studies of the molecular bases underlying fruit body development. Truffles, however, are not obligate symbionts, and some of them, including Tuber borchii, can be grown in pure mycelial cultures by exploitation of their limited saprotrophic...

show abstract

Comparative Analysis of Sequences Expressed during the Liquid-Cultured Mycelia and Fruit Body Stages of Pleurotus ostreatus

Cited by 59 publications

References 26 publications

Soybean Rust: Five Years of Research

Soybean Rust: Five Years of Research

Gene expression profiling of a Zn-tolerant and a Zn-sensitive Suillus luteus isolate exposed to increased external zinc concentrations

Isolation and Characterization of Differentially Expressed Genes in the Mycelium and Fruit Body of Tuber borchii

Contact Info

Product

Resources

About