A multiple signal amplification sandwich-type SERS biosensor for femtomolar detection of miRNA

Programmable mismatch-fueled high-efficiency DNA signal converter

“…Moreover, the detection of tumour-specic circulating miRNA at ultrahigh sensitivity is of utmost signicance for the early diagnosis and monitoring of cancer; 34 thus, there is a great need to develop new approaches or sensing media with improved miRNA detection limits owing to its low abundance in total RNA samples and the susceptibility to degradation. [35][36][37] In view of this, we indeed explored a practically valuable HEEFTRA.…”

Gold nanomaterials for optical biosensing and bioimaging

“…108 Thus, numerous attempts have been made to fabricate AuNP monolayers with desired density and precisely controlled nanogaps. [109][110][111] For example, Shao et al fabricated a multiple signal amplication sandwich type SERS biosensor for the detection of miRNA with a detection limit of 3 fM. A triple signal amplication was achieved by using AuNPs of selective morphology, hybridization chain reaction amplication and using silver nanoparticles in combination with AuNPs.…”

Nucleic Acids Analysis

“…Owing to the high stability, low cost, and convenient labeling, nanoparticles can also serve as signal probes for DNA detection to further improve the performance of sandwich assays [106][107][108][109][110][111][112][113][114][115]. For example, Zhang et al [116] reported a nanoparticle based "sandwich" detection strategy by immobilizing gold nanoparticles (AuNPs) with reporter probes that flank the target nucleic acids.…”