Programmable mismatch-fueled high-efficiency DNA signal converter

Zhang, Xiaolong; Yang, Zhehan; Chang, Yuanyuan; Liu, Di; Li, Yun-Rui; Chai, Yaqin; Zhuo, Ying; Yuan, Ruo

doi:10.1039/c9sc05084a

Cited by 29 publications

(18 citation statements)

References 50 publications

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“…The sensor eliminates the need to immobilize the recognition probe on the electrode surface, dramatically simplifies the electrode preparation step, and exhibits a signal-enhanced output pattern. Interestingly, the DNA tetrahedra-based three-stranded nucleic acid in our designed biosensor has an extreme dependence on pH, making the constructed biosensor reproducible [38] . This is crucial for the effective implementation of SARS-CoV-2 detection in areas where resources are currently relatively scarce.…”

Section: Introductionmentioning

confidence: 99%

A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a trans-activity

Zhang¹,

Fan²,

Ding³

et al. 2022

Chemical Engineering Journal

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In this work, we constructed an exonuclease III cleavage reaction-based isothermal amplification of nucleic acids with CRISPR/Cas12a-mediated pH-induced regenerative Electrochemiluminescence (ECL) biosensor for ultrasensitive and specific detection of SARS-CoV-2 nucleic acids for SARS-CoV-2 diagnosis. The triple-stranded nucleic acid in this biosensor has an extreme dependence on pH, which makes our constructed biosensor reproducible. This is essential for effective large-scale screening of SARS-CoV-2 in areas where resources are currently relatively scarce. Using this pH-induced regenerative biosensor, we detected the SARS-CoV-2 RdRp gene with a detection limit of 43.70 aM. In addition, the detection system has good stability and reproducibility, and we expect that this method may provide a potential platform for the diagnosis of COVID-19.

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Section: Introductionmentioning

confidence: 99%

A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a trans-activity

Zhang¹,

Fan²,

Ding³

et al. 2022

Chemical Engineering Journal

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“…34 Furthermore, the amplification process is driven by the increase of entropy and it has excellent discriminating ability toward single-base mutations according to the difference in the base pair breathing rate. 35,36 Therefore, it has excellent selectivity for the target detection.…”

mentioning

confidence: 99%

“…In addition, the double-stranded waste is generated after the reaction, which makes the reaction irreversible, thereby avoiding the interference of the complex environment and increasing the stability of this reaction . Furthermore, the amplification process is driven by the increase of entropy and it has excellent discriminating ability toward single-base mutations according to the difference in the base pair breathing rate. , Therefore, it has excellent selectivity for the target detection.…”

mentioning

confidence: 99%

Electrochemiluminescence Biosensor Based on Entropy-Driven Amplification and a Tetrahedral DNA Nanostructure for miRNA-133a Detection

Zhu

Yan

et al. 2021

Anal. Chem.

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The early and rapid diagnosis of acute myocardial infarction (AMI) is of great significance to its treatment. Here, we developed an electrochemiluminescence biosensor based on an entropy-driven strand displacement reaction (ETSD) and a tetrahedral DNA nanostructure (TDN) for the detection of the potential AMI biomarker microRNA-133a. In the presence of the target, numerous Ru(bpy)3 2+-labeled signal probes (SP) were released from the preformed three-strand complexes through the process of ETSD. The ETSD reaction cycle greatly amplified the input signal of the target. The released SP could be captured by the TDN-engineered biosensing interface to generate a strong ECL signal. The rigid structure of TDN could significantly improve the hybridization efficiency. With the assistant of double amplification of TDN and ETSD, the developed biosensor has a good linear response ranging from 1 fM to 1 nM for microRNA-133a, and the detection limit is 0.33 fM. Additionally, the constructed biosensor has excellent repeatability and selectivity, demonstrating that the biosensor possesses a great application prospect in clinical diagnosis.

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“…As a proof-of-concept, the BCNO QDs/S 2 O 8 2– ECL system has been employed for ultrasensitive determination of microRNA-21, whose abnormal expression is closely related to the occurrence and development of cancers . To assess the analytical performance of the biosensor, diverse concentrations of microRNA-21 were measured based on this proposed method.…”

Section: Resultsmentioning

confidence: 99%

High-Efficient Electrochemiluminescence of BCNO Quantum Dot-Equipped Boron Active Sites with Unexpected Catalysis for Ultrasensitive Detection of MicroRNA

et al. 2020

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Herein, the boron radical active sites of boron carbon oxynitride quantum dots (BCNO QDs) are electrically excited to produce boron radicals (B•) for catalyzing peroxydisulfate (S2O8 2–) as a coreactant to accelerate the generation of abundant sulfate radicals (SO4 •–) for significant enhancement in the electrochemiluminescence (ECL) efficiency of BCNO QDs, which overcome the defect of traditional carbon-based QDs with low ECL efficiency. Impressively, under extremely low concentration of S2O8 2– solution, the BCNO QDs/S2O8 2– system could exhibit high ECL emission, realizing environmental friendliness and excellent biocompatibility for sensitive bioanalysis. As a proof-of-concept, BCNO QDs, a new generation of ECL emitters with high ECL efficiency, were successfully used in the ultrasensitive determination of microRNA-21, which pushes the exploration of new ECL emitters and broadens the application in the field of clinical diagnosis, ECL imaging, and molecular devices.

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Programmable mismatch-fueled high-efficiency DNA signal converter

Abstract: Herein, by directly introducing mismatched reactant DNA, high-reactivity and high-threshold enzyme-free target recycling amplification (EFTRA) is explored.

Cited by 29 publications

References 50 publications

A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a trans-activity

A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a trans-activity

Electrochemiluminescence Biosensor Based on Entropy-Driven Amplification and a Tetrahedral DNA Nanostructure for miRNA-133a Detection

High-Efficient Electrochemiluminescence of BCNO Quantum Dot-Equipped Boron Active Sites with Unexpected Catalysis for Ultrasensitive Detection of MicroRNA

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