The
early and rapid diagnosis of acute myocardial infarction (AMI)
is of great significance to its treatment. Here, we developed an electrochemiluminescence
biosensor based on an entropy-driven strand displacement reaction
(ETSD) and a tetrahedral DNA nanostructure (TDN) for the detection
of the potential AMI biomarker microRNA-133a. In the presence of the
target, numerous Ru(bpy)3
2+-labeled signal probes
(SP) were released from the preformed three-strand complexes through
the process of ETSD. The ETSD reaction cycle greatly amplified the
input signal of the target. The released SP could be captured by the
TDN-engineered biosensing interface to generate a strong ECL signal.
The rigid structure of TDN could significantly improve the hybridization
efficiency. With the assistant of double amplification of TDN and
ETSD, the developed biosensor has a good linear response ranging from
1 fM to 1 nM for microRNA-133a, and the detection limit is 0.33 fM.
Additionally, the constructed biosensor has excellent repeatability
and selectivity, demonstrating that the biosensor possesses a great
application prospect in clinical diagnosis.
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