A multidimensional concept for mercury neuronal and sensory toxicity in fish - From toxicokinetics and biochemistry to morphometry and behavior

Pereira, Patrícia; Korbas, Małgorzata; Pereira, Vitória; Cappello, Tiziana; Canário, João; Almeida, Armando; Pacheco, Mário

doi:10.1016/j.bbagen.2019.01.020

Cited by 49 publications

(27 citation statements)

References 282 publications

(586 reference statements)

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“…Metalothioneines and low molecular weight thiols are important factors to mediate the transport and distribution of Hg. The mechanisms of transport and accumulation Hg species in brain cells remain discussable (Aschner and Aschner 1990;Pereira et al, 2019). Number data support the opinion that inorganic Hg compounds cannot cross BBB.…”

Section: Introductionmentioning

confidence: 79%

Sublethal Doses of Inorganic Mercury Induce Dose-Depended Upregulation of RPA1 Content and Inhibit p53 Expression in the Brain of Rainbow Trout (Oncorhynchus mykiss)

Taysi¹,

Söğüt²,

Nedzvetsky³

et al. 2019

Türk Tarım Ve Doğa Bilimleri Dergisi

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Mercury (Hg) is one of most toxic and widespread element of aquatic environment. Almost every kind of the fish can accumulate Hg. Hg-induced peculiarities of cellular malfunction could be used as adequate biomarker to estimate the contamination risk in polluted aquatic ecosystems. The brain cells are high susceptible to the Hg compounds cytotoxicity. Various Hg species have different harmful effects on both structure and function of the brain cells. Neurotoxicity of inorganic Hg remains discussable and studied restrictedly. In this study, we have studied the role of RPA1 and p53 proteins in brain cell response to sublehtal (25% LD 50 and 50% LD 50) doses of inorganic Hg in rainbow trout (Oncorhynchus mykiss). LD 50 value of Hg chloride in presented study was determined as 551 µg/L relate to 96 hours exposure. Two sublethal doses were used in the exposure rainbow trout at 2 and 7 days. The treatment with Hg chloride induced in fish brain dose-dependent increase in ROS level as well as time-dependent growth. Moreover, the exposure to both 25% and 50% LD 50 Hg doses have caused significant upregulation of RPA1 expression. In the brain tissue of fish exposed to Hg for 2 days, it stimulated slightly expression of p53. Contrary, 7 days exposure induced significant decrease in p53 expression. The results of presented study evidence that sublethal doses of inorganic Hg are extremely neurotoxic and can induce in the fish brain signaling pathways disturbance through decline of stress sensor protein p53. Besides, the increase in RPA1 expression let to assume that brain cells of the fish can repair ROS-induced DNA breaks and prevent genotoxic effect of inorganic Hg. Overall, current data pointed out that inorganic mercury is high toxic to fish brain cells and this question requires future research.

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Section: Introductionmentioning

confidence: 79%

Sublethal Doses of Inorganic Mercury Induce Dose-Depended Upregulation of RPA1 Content and Inhibit p53 Expression in the Brain of Rainbow Trout (Oncorhynchus mykiss)

Taysi¹,

Söğüt²,

Nedzvetsky³

et al. 2019

Türk Tarım Ve Doğa Bilimleri Dergisi

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“…Casein 1 27.0 Fish meal 2 32.0 Wheat flour 3 18.0 Dextrin 1 4.20 Corn starch 2 3.50 Fish oil 4 6.10 Vitamin premix 5 3.00 Mineral premix 6 3.00 Cellulose 1 3.20 Hg-premix (5000 ppm) 0.00…”

Section: Ingredients % In Dietmentioning

confidence: 99%

“…(2) inorganic mercury compounds, including mercurous chloride (Hg 2 Cl 2 ), mercuric chloride (HgCl 2 ) and mercuric sulfide (HgS); and (3) organic mercury compound, especially methylmercury (MeHg) [3]. All forms of mercury are toxic [4], and organic MeHg is neurotoxic compared to inorganic HgCl 2 in fish [5]. Although mercury is a naturally occurring element, human or anthropogenic activities, particularly through industrial processes, have led to widespread distribution of toxic mercurial derivatives throughout the biosphere [6].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Dietary Organic and Inorganic Mercury Threshold Levels on Induced Mercury Toxicity in a Marine Fish Model

Raihan

Moniruzzaman

Park

et al. 2020

Animals

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Mercury as one of the most toxic elements can be present in organic or inorganic form in marine fishes, which may cause a potential threat to public health. In this study, we investigated to determine the dietary organic (O-Hg) and inorganic (I-Hg) mercury threshold levels on induced mercury toxicity in juvenile olive flounder, Paralichthys olivaceus as a marine fish model. Twenty-eight fish averaging 3.1 ± 0.05 g (mean ± SD) were arbitrarily assigned to each of 27 tanks. Each tank was arbitrarily restricted to triplicates of nine experimental diets for eight weeks. The experimental diets were manufactured to contain 0 (Control), 10 (I-Hg10, O-Hg10), 20 (I-Hg20, O-Hg20), 40 (I-Hg40, O-Hg40) and 160 (I-Hg160, O-Hg160) mg/kg diet in organic form as methylmercury (MeHg) or in inorganic form as mercuric chloride (HgCl2). At the termination of the experimental trial, weight gains (WGs) of fish fed the control and 10 (I-Hg10, O-Hg10) diets were remarkably higher than those of fish fed the 20 (I-Hg20, O-Hg20), 40 (I-Hg40, O-Hg40) and 160 (I-Hg160, O-Hg160) (p < 0.05). Specific growth rate and feed efficiency of fish fed control and 10 (I-Hg10, O-Hg10) diets were significantly higher than those of fish fed 40 (I-Hg40, O-Hg40) and 160 (I-Hg160, O-Hg160) diets. In comparison to the dietary inorganic mercury, dietary MeHg bioaccumulation rates were significantly higher in the tissue levels according to the dietary inclusion levels. MeHg accumulated mostly in kidney, followed by liver and gill tissues. HgCl2 accumulated in tissues, in decreasing order, liver > kidney > gills. A broken-line regression model for percentage of WG indicated that the threshold toxicity level for an Hg-incorporated diet of juvenile olive flounder could be 13.5 mg Hg/kg in the form of HgCl2 and 8.7 mg Hg/kg in the form of MeHg.

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“…The cytoskeleton proteins, reparative protein A (RPA70), transcriptional regulator nuclear factor kappa-B cells (NF-kB) and DNA damage repairing poly-(ADP-ribose)-polymerase (PARP) were reported as valid biomarkers to estimate aluminium, cadmium, mercury and copper neurotoxicity (Novitsky et al, 2013;Nedzvetsky et al, 2018;Kirici et al, 2019). The study of the effect environmental toxicity on the brain is one of the relevant and major areas of research (Legradi et al, 2018;Pereira et al, 2019;Taysi et al, 2019). Besides, brain cells are critically susceptible to most pollutants, which induce irreversible metabolic and structural changes in it (Nedzvetsky et al, 2006, Taysi et al, 2019.…”

Section: Introductionmentioning

confidence: 99%

“…Besides, brain cells are critically susceptible to most pollutants, which induce irreversible metabolic and structural changes in it (Nedzvetsky et al, 2006, Taysi et al, 2019. Thus, the central nervous system represents a potential target for any ecotoxicant impact (Nedzvetsky et al, 2006;Pereira et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Local industrial pollution induces astrocyte cytoskeleton rearrangement in the dice snake brain: GFAP as a biomarker

et al. 2020

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The present study was designed to evaluate the responsiveness of modulation of glial fibrillary acidic protein (GFAP) content and its fragmentation in the snake brain as a biomarker of local industrial pollution of aquatic ecosystems. Despite GFAP being a well known cytoskeleton marker of astrocytes’ reactivity in the brain of vertebrates, its expression in the snake brain remains insufficiently described. The GFAP expression and its fragmentation were detected using the immunoblot method in the snake brain. ROS level was determined with dichlorofluorescein diacetate fluorescence. The content of the glial fibrillary acidic protein (GFAP) of filament (cytoskeleton) and soluble (cytosol) fractions in the brain of dice snake Natrix tessellata from three ecosystems with different rates of industrial pollution were studied (two polluted and one clean control site). Characteristic increase in GFAP fragmentation was noted for the snakes from both the researched polluted sites. Significant increase in the content of the GFAP cleaved polypeptide fragments induced by industrial pollution exposure was confirmed in the snakes’ brains. Meaningful GFAP fragmentation was determined in snake brain astrocytes as an increase in cleaved fragments of 47–35 kDa molecular weight for both soluble and cytoskeletal GFAP fractions. We found significant abnormality in the ratio of the GFAP soluble fraction to the cytoskeletal one in contaminant-exposed dice snakes. It should testify to significant metabolic disturbance in nerve cells of the dice snakes. Furthermore, growth of reactive oxygen species level as the main cause of oxidative stress was determined in brains of the snakes exposed to environmental toxicity. Thus, astrocyte cytoskeleton disorders are associated with pollutant-induced redox imbalance in the snake brain. Despite the limited data on glial cell biology in the reptilian brain, the observed results prove that snake astrocytes can respond to the environmental toxicity using typical astroglial response. The presented results evidence that monitoring of molecular characteristics of glial cytoskeleton in dice snakes could be used as reliable biomarker of neurotoxicity and adverse effects of industrial pollution. Further studies are required to elucidate the role of astrocyte cytoskeleton in the response against neurotoxic contaminants.

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A multidimensional concept for mercury neuronal and sensory toxicity in fish - From toxicokinetics and biochemistry to morphometry and behavior

Cited by 49 publications

References 282 publications

Sublethal Doses of Inorganic Mercury Induce Dose-Depended Upregulation of RPA1 Content and Inhibit p53 Expression in the Brain of Rainbow Trout (Oncorhynchus mykiss)

Sublethal Doses of Inorganic Mercury Induce Dose-Depended Upregulation of RPA1 Content and Inhibit p53 Expression in the Brain of Rainbow Trout (Oncorhynchus mykiss)

Evaluation of Dietary Organic and Inorganic Mercury Threshold Levels on Induced Mercury Toxicity in a Marine Fish Model

Local industrial pollution induces astrocyte cytoskeleton rearrangement in the dice snake brain: GFAP as a biomarker

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