A Multianalyte Electrochemical Genosensor for the Detection of High-Risk HPV Genotypes in Oral and Cervical Cancers

Chaibun, Thanyarat; Thanasapburachot, Patcharanin; Chatchawal, Patutong; Yin, Lee Su; Jiaranuchart, Sirimanas; Jearanaikoon, Patcharee; Promptmas, Chamras; Buajeeb, Waranun; Lertanantawong, Benchaporn

doi:10.3390/bios12050290

Cited by 9 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Instead, many of them use “spiking” whereby synthetic sequences are inserted into the serum to show the percentage of recovered sequences and thus to evaluate possible matrix effects. Many nanomaterial‐based papers that actually used cervical samples from women tested their assays on a relatively low number of patients 185–190 . For instance, Hong et al employed carboxylic group‐functionalized magnetic nanoparticles for split electrochemiluminesce assay for detection of HPV16 based on gold nanocluster probes.…”

Section: Nanomaterials Enhancing Assay Sensitivitymentioning

confidence: 99%

See 1 more Smart Citation

Advanced technologies towards improved HPV diagnostics

Bartosik,

Moranova,

Izadi

et al. 2024

Journal of Medical Virology

View full text Add to dashboard Cite

Persistent infection with high‐risk types of human papillomaviruses (HPV) is a major cause of cervical cancer, and an important factor in other malignancies, for example, head and neck cancer. Despite recent progress in screening and vaccination, the incidence and mortality are still relatively high, especially in low‐income countries. The mortality and financial burden associated with the treatment could be decreased if a simple, rapid, and inexpensive technology for HPV testing becomes available, targeting individuals for further monitoring with increased risk of developing cancer. Commercial HPV tests available in the market are often relatively expensive, time‐consuming, and require sophisticated instrumentation, which limits their more widespread utilization. To address these challenges, novel technologies are being implemented also for HPV diagnostics that include for example, isothermal amplification techniques, lateral flow assays, CRISPR‐Cas‐based systems, as well as microfluidics, paperfluidics and lab‐on‐a‐chip devices, ideal for point‐of‐care testing in decentralized settings. In this review, we first evaluate current commercial HPV tests, followed by a description of advanced technologies, explanation of their principles, critical evaluation of their strengths and weaknesses, and suggestions for their possible implementation into medical diagnostics.

show abstract

Section: Nanomaterials Enhancing Assay Sensitivitymentioning

confidence: 99%

“…Many nanomaterial-based papers that actually used cervical samples from women tested their assays on a relatively low number of patients. [185][186][187][188][189][190] For instance, Hong et al…”

Section: Sensitivitymentioning

confidence: 99%

Advanced technologies towards improved HPV diagnostics

Bartosik,

Moranova,

Izadi

et al. 2024

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“…Biosensors are being designed and developed by researchers and scientists to identify disease signs, prevent dental decay, periodontal disease, and detect early malignancies. It is possible to successfully identify endemic oral diseases at an early stage using electrochemical biosensors (Chaibun et al, 2022). Studies, for example, have shown that nucleic acid and protein biosensors may detect common oral illnesses, giving significant information for the development of non-invasive detection (Chakraborty et al, 2022).…”

Section: Electrochemical Biosensors Principle and Classificationmentioning

confidence: 99%

“…Infection with high-risk human papillomavirus (HPV) is a major risk factor for oral and cervical cancers (Zheng et al, 2016). Hence, Chaibun et al developed a multianalyte electrochemical DNA biosensor to detect the high-risk HPV genotypes 16 and 18 (Chaibun et al, 2022). The assay involves the sandwich hybridization of the HPV target to the silica-redox dye reporter probe and capture probe, followed by electrochemical detection.…”

Section: Composition Of Microorganismsmentioning

confidence: 99%

Advances in electrochemical biosensors for the detection of common oral diseases

Han

Liu

Guan

et al. 2023

Preprint

View full text Add to dashboard Cite

With the advancement of society and people’s knowledge of oral health, the prevention of oral illnesses such as dental caries, periodontal disease, oral cancer, oral mucosal disease, salivary gland disease, and so on has become the primary focus of current health status and treatment. Although treatment for these diseases has focused on removing lesions or inhibiting progression, early detection has always been the focus of clinical research. And the non-invasive diagnosis is now the primary goal of clinical diagnosis. Meanwhile, the electrochemical approach can satisfy this need while also achieving a speedy and reliable diagnosis, giving patients a more comfortable option. And noninvasive detection of specific diseases can be achieved by detecting markers carried in body fluids. Therefore, in the context of disease, pathophysiological progress and electrochemical-assisted diagnosis form a close relationship, providing a basis for the detection of oral disease related indicators. As a result, this review comprehensively summarizes the detection and diagnosis of common oral diseases using electrochemical sensing technology and provides important insights for the development of electrochemical biosensors for the early detection of common oral diseases.

show abstract

“…Among emerging technologies available, DNA biosensor, an analytical device incorporating a single-stranded oligonucleotide (probe) linked with a physicochemical transducer, offers an interesting alternative test. In recent years, this technology has been studied widely as a potential novel method for the detection of DNA hybridization in various fields such as the diagnosis of diseases including cancer [ 25 , 26 , 27 ], the detection of infectious agents [ 28 , 29 ], drug screening [ 30 , 31 , 32 ], crops screening [ 33 , 34 ], and forensic applications [ 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii

et al. 2022

View full text Add to dashboard Cite

Acinetobacter baumannii (A. baumannii) are phenotypically indistinguishable from the Acinetobacter calcoaceticus–A. baumannii (ACB) complex members using routine laboratory methods. Early diagnosis plays an important role in controlling A. baumannii infections and this could be assisted by the development of a rapid, yet sensitive diagnostic test. In this study, we developed an enzyme-based electrochemical genosensor for asymmetric PCR (aPCR) amplicon detection of the blaOXA-51-like gene in A. baumannii. A. baumannii blaOXA-51-like gene PCR primers were designed, having the reverse primer modified at the 5′ end with FAM. A blaOXA-51-like gene sequence-specific biotin labelled capture probe was designed and immobilized using a synthetic oligomer (FAM-labelled) deposited on the working electrode of a streptavidin-modified, screen-printed carbon electrode (SPCE). The zot gene was used as an internal control with biotin and FAM labelled as forward and reverse primers, respectively. The blaOXA-51-like gene was amplified using asymmetric PCR (aPCR) to generate single-stranded amplicons that were detected using the designed SPCE. The amperometric current response was detected with a peroxidase-conjugated, anti-fluorescein antibody. The assay was tested using reference and clinical A. baumannii strains and other nosocomial bacteria. The analytical sensitivity of the assay at the genomic level and bacterial cell level was 0.5 pg/mL (1.443 µA) and 103 CFU/mL, respectively. The assay was 100% specific and sensitive for A. baumannii. Based on accelerated stability performance, the developed genosensor was stable for 1.6 years when stored at 4 °C and up to 28 days at >25 °C. The developed electrochemical genosensor is specific and sensitive and could be useful for rapid, accurate diagnosis of A. baumannii infections even in temperate regions.

show abstract

A Multianalyte Electrochemical Genosensor for the Detection of High-Risk HPV Genotypes in Oral and Cervical Cancers

Cited by 9 publications

References 31 publications

Advanced technologies towards improved HPV diagnostics

Advanced technologies towards improved HPV diagnostics

Advances in electrochemical biosensors for the detection of common oral diseases

Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii

Contact Info

Product

Resources

About