A monolithic silicon based integrated signal generation and detection system for monitoring DNA hybridisation

Bertolino, Chiara A.; MacSweeney, Marion; Tobin, Jane; O'Neill, B.; Sheehan, Michelle M.; Coluccia, Salvatore; Berney, Helen

doi:10.1016/j.bios.2004.12.007

Cited by 37 publications

(20 citation statements)

References 31 publications

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“…In addition, it shares characteristics of electrochemical methods, such as highly localized reaction and spacial control. However, only a few examples of heterogeneous ECL-based hybridization assays have been reported (Miao and Bard, 2003;Bertolino et al, 2005). Miao and Bard (2003) performed an ECL assay on gold using 23-mer oligonucleotides.…”

Section: Introductionmentioning

confidence: 99%

“…Miao and Bard (2003) performed an ECL assay on gold using 23-mer oligonucleotides. Bertolino et al (2005) fabricated a silicon based ECL chip with interdigitated gold electrodes and integrated photodiode. The system was able to discriminate 25% mismatched strands.…”

Section: Introductionmentioning

confidence: 99%

“…Wong et al, 2005), microgravimetrical (Su et al, 2004), bioluminescence (Kricka, 1999), chemiluminescence (Cheek et al, 2001) and electrochemiluminescence (ECL) (Miao and Bard, 2003;Spehar et al, 2004;Bertolino et al, 2005;Firrao, 2005) methods have been used.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemiluminescent hybridization chip with electric field aided mismatch discrimination

Spehar‐Délèze

Schmidt

Neier

et al. 2006

Biosensors and Bioelectronics

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This paper describes a heterogeneous DNA-hybridization assay based on electrochemiluminescence (ECL) detection on gold electrodes. Short, 15-mer oligonucleotides were conjugated with a synthesized electrochemiluminescent label, bis(2,2 -bipyridine)-5-isothiocyanato-1,10-phenanthroline ruthenium(II) at the amino-modified 5 -end. Gold electrodes were derivatized with 15-mer oligonucleotide probes via 1-(3-(dimethylamino)propyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) cross-linking reaction and hybridized with Ru-labeled strands. Two types of self-assembled-monolayers have been utilized for the immobilization reaction, 3-mercaptopropanoic acid (3-MHA) and 16-mercaptohexadecanoic acid (16-MHA). Longer thiols were more stable at high electrode potentials needed for the ECL generation. The system was sensitive down to 1 fmol of labeled complementary strand, detected in 30 L of buffer. Mismatch discrimination was achieved both passively by washing and actively by application of negative electrode potential on electrodes prior to detection, but active denaturing lead to better results. Two base-pair mismatches were discriminated at room temperature.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrochemiluminescent hybridization chip with electric field aided mismatch discrimination

Spehar‐Délèze

Schmidt

Neier

et al. 2006

Biosensors and Bioelectronics

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show abstract

“…Of particular interest is the detection of DNA strands of specific sequence due to applications in forensic science, food analysis, pharmacology, genomics, etc. Most DNA hybridization assays rely on fluorescence detection, but other methods, such as quartz crystal microbalance [1], electrochemistry [2,3], chemiluminescence [4] and anodic electrochemiluminescence (ECL) [5][6][7][8][9] have also been applied.…”

Section: Introductionmentioning

confidence: 99%

“…In anodic ECL, an electrochemiluminescent luminophore (usually tris(bipyridine)ruthenium, Ru(bpy) 3 2+ ) is oxidized on a surface of a metal or carbon electrode and light is generated upon reaction with a coreactant (typically ntripropylamine) [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence of a rhodamine label at oxide-coated aluminum and silicon electrodes

Spehar‐Délèze

Suomi²,

Jiang³

et al. 2006

Electrochimica Acta

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This paper describes a heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence (HECL) of a rhodamine label. Thin oxide-film coated aluminum and silicon electrodes were modified with an aminosilane layer and derivatized with short, 15-mer oligonucleotides via diisothiocyanate coupling. Target oligonucleotides were conjugated with tetramethylrhodamine (TAMRA) dye at their amino modified 5 end and hybridization was detected using HECL of TAMRA. Preliminary results indicate sensitivity down to picomolar level and low nonspecific adsorption. The sensitivity was better on oxide-coated silicon compared to oxide-coated aluminum electrodes and two-base pair mismatched hybrids were successfully discriminated. The experimental results presented here might be useful for the design of disposable electrochemiluminescent DNA biosensors.

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Electrochemiluminescent Systems as Devices and Sensors

Kapturkiewicz¹

2009

Electrochemistry of Functional Supramolecular Systems

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A monolithic silicon based integrated signal generation and detection system for monitoring DNA hybridisation

Cited by 37 publications

References 31 publications

Electrochemiluminescent hybridization chip with electric field aided mismatch discrimination

Electrochemiluminescent hybridization chip with electric field aided mismatch discrimination

Heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence of a rhodamine label at oxide-coated aluminum and silicon electrodes

Electrochemiluminescent Systems as Devices and Sensors

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