Heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence of a rhodamine label at oxide-coated aluminum and silicon electrodes

Spehar‐Délèze, Anna‐Maria; Suomi, Johanna; Jiang, Qinghong; Rooij, N. F. de; Koudelka‐Hep, M.; Kulmala, Sakari

doi:10.1016/j.electacta.2006.02.017

Cited by 12 publications

(6 citation statements)

References 35 publications

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“…Other methods of DNA hybridization detection include luminol−H 2 O 2 -based , and hot electron-induced ECL . A luminol derivative, N -(4-aminobutyl)- N -etylisoluminol (ABEI), anchored on a target ssDNA was used for the ECL detection of the hybridization between a complementary probe ssDNA covalently linked to a polypyrrole support and the target ssDNA .…”

Section: Biorelated Applicationsmentioning

confidence: 99%

“…501 Other methods of DNA hybridization detection include luminol-H 2 O 2 -based 502,503 and hot electron-induced ECL. 504 A luminol derivative, N-(4-aminobutyl)-N-etylisoluminol (ABEI), anchored on a target ssDNA was used for the ECL detection of the hybridization between a complementary probe ssDNA covalently linked to a polypyrrole support and the target ssDNA. 503 In the latter case, thin oxide film coated Al and Si electrodes were modified with an aminosilane layer and derivatized with short, 15-mer DNAs via diisothiocyanate coupling.…”

Section: Dna Detection and Quantificationmentioning

confidence: 99%

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Electrogenerated Chemiluminescence and Its Biorelated Applications

2008

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Wujian Miao received his undergraduate diploma in chemistry from Nantong University (Nantong, China) in 1982, his M.Sc. degree in analytical chemistry from Zhongshan University (Guangzhou, China, with Jinyuan Mo) in 1991, and his Ph.D. degree in electrochemistry from Monash University (Melbourne, Australia, with Alan M. Bond) in 2000. He then served as a Research Scientist in CSIRO (Melbourne, Australia), followed by a postdoctoral fellowship at the University of Texas at Austin with Allen J. Bard in 2001. Since 2004 he has served as an assistant professor of chemistry at the University of Southern Mississippi.

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Section: Biorelated Applicationsmentioning

confidence: 99%

Section: Dna Detection and Quantificationmentioning

confidence: 99%

Electrogenerated Chemiluminescence and Its Biorelated Applications

2008

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“…Luminol-H 2 O 2 -based method [148,149] and hot electron-induced ECL [150] have also been reported. In the former case, immobilization of N-(4-aminobutyl)-N-etylisoluminol (ABEI), a luminol derivative, on a target ssDNA was employed for the ECL detection of the hybridization between a complementary probe ssDNA covalently linked to a polypyrrole support and the target ssDNA [149].…”

Section: Dna Detection and Quantificationmentioning

confidence: 97%

Applications of Electrochemiluminescence

Parveen

Aslam

et al. 2013

SpringerBriefs in Molecular Science

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The development of electrochemiluminescence (ECL) applications is a growing field, having the potential advantages of ECL over conventional chemiluminescence. ECL has found various applications in immunoassays, DNA probe assays, and aptasensors by employing ECL-active species as labels on biological molecules. The recent use of ECL to detect many chemically, biochemically, clinically, and environmentally important analytes is reviewed.

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“…[16][17][18][19][20][21][22][23][24] Many fluorophores have been applied in the biosensors development by optical transduction, such as cyanine, rhodamine, and alexa fluor, [25][26][27] however, quantum dots (QD) are promising substitutes for these fluorophores and recently have received world attention. [28][29][30][31][32][33][34][35] QD are semiconductors materials that exhibit extraordinary optical properties, such as broad absorption spectra, narrow and symmetric emission spectrum, high photostability and decay time. 36 These exceptional photophysical properties can be applied for biosensors assembly, aiming detection of human diseases, for example, botulism, hepatitis B and breast cancer.…”

Section: Introductionmentioning

confidence: 99%

Bioelectrode Applied to Diagnosis of Cardiac Disease

Lp¹,

Ferreira²,

Af³

et al. 2014

j nanosci nanotechnol

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This paper describes the assembly of a bioelectrode based on poly(3-aminophenol) and anti-troponin T antibody for recognition of troponin T, which is a specific biomarker for diagnosis of acute myocardial infarction. This disease causes loss of cellular components, allowing the output of molecules such as troponin T. This proteic component acts as biomarker for diagnosis of acute myocardial infarction due to their high sensitivity and specificity. Poly(3-aminophenol) was electrodeposited onto fluorine doped tin oxide (FTO) coated glass and characterized by spectroscopic methods (UV-Visible, fluorescence, infrared), electrochemical methods (cyclic voltammetry and electrochemical impedance spectroscopy) and morphological methods (laser interferometry, field emission scanning electronic microscopy, and atomic force microscopy). UV/Vis analysis indicated that poly(3-aminophenol) presents extension of conjugation, in according with fluorescence studies. Electrochemical studies indicated that poly(3-aminophenol) electrodeposited in FTO is a material with passivating characteristics for anions and capacity of retaining cationic compounds. Laser interferometry showed that poly(3-aminophenol) covers the FTO surface with a thickness off 375 ± 75 nm. Surface images by FE-SEM and AFM have shown a full coverage on the FTO by the polymer film. The incorporation of anti-troponin T antibody on FTO electrode modified with poly(3-aminophenol) allowed effective and selective detection of cardiac biomarker troponin T, by electrochemical impedance spectroscopy (label free) and by photoluminescence, based on CdSe/ZnS quantum dots. This research shows the step by step assembly of the bioelectrode, used for detection of troponin T by impedimetric and fluorescence methods, opening the opportunity for its use in the diagnosis of others diseases.

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Heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence of a rhodamine label at oxide-coated aluminum and silicon electrodes

Cited by 12 publications

References 35 publications

Electrogenerated Chemiluminescence and Its Biorelated Applications

Electrogenerated Chemiluminescence and Its Biorelated Applications

Applications of Electrochemiluminescence

Bioelectrode Applied to Diagnosis of Cardiac Disease

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