A monoclonal antibody-enzyme immunoassay for serum carcinoembryonic antigen with increased specificity for carcinomas.

Hedin, A.; Carlsson, Leif; Berglund, Asta; Hammarström, Sten

doi:10.1073/pnas.80.11.3470

Cited by 49 publications

(12 citation statements)

References 15 publications

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“…Antibodies, bought or self-established, should prior to utilization be properly examined with respect to sensitivity and specificity for the application planned. The anti-CEA total antibody has been extensively investigated in collaborative epitope screening projects [97,229], and has been subject to studies of differential CEA antibody specificity, and cross-reactivity with other antigens like NCA, and NCA-2 has also been extensively examined [115,230,231]. The T84.66 antibody was shown to be specific for full-length CEA, not recognizing the shorter, C-terminally truncated versions [229].…”

Section: B) Antibody Specificitymentioning

confidence: 99%

“…NCA-2 and CEA are identical in the amino terminal sequence [112] and both are highly glycosylated, but they differ in glycosylation patterns [113,114] which might influence conformational stability and protease resistance [113,114]. Studies on the cross reactivity of NCA-2 has resulted in improved CEA assays that do not recognize NCA-2 [115] but still little is known about the specificity of NCA-2 antibodies in different experimental settings, and it has as far as we know not been explored for clinical associations in a large patient cohort [116][117][118]. [119,120].…”

Section: G) Predictive Biomarkersmentioning

confidence: 99%

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Investigation of nonspecific cross-reacting antigen 2 as a prognostic biomarker in bone marrow plasma from colorectal cancer patients

et al. 2011

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Section: B) Antibody Specificitymentioning

confidence: 99%

Section: G) Predictive Biomarkersmentioning

confidence: 99%

Investigation of nonspecific cross-reacting antigen 2 as a prognostic biomarker in bone marrow plasma from colorectal cancer patients

et al. 2011

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“…Application of the monoclonal antibody technique to the quantitative measurement of serum markers in patients with several diseases has been described (Wands et al, 1982;Hedin et al, 1983). Bellet et al (1984) recently reported the specific radioimmunoassay method for hepatocellular carcinoma with use of monoclonal anti-AFP antibodies which were supposed to recognize certain unique epitopes of AFP.…”

Section: Discussionmentioning

confidence: 99%

Close topographical relationship in alpha foetoprotein (AFP) between a lens culinaris binding glycan and the epitope recognized by AFP-reactive monoclonal antibody, 18H4

Suzuki¹,

Aoyagi²,

Muramatsu³

et al. 1987

Br J Cancer

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Summary Monoclonal antibodies 18H4 and 19F12 against alpha-foetoprotein (AFP) were examined by enzyme immunoassay for binding to two forms of AFP that were separated on the basis of the reactivity with lentil lectin (LCA). LCA-binding and LCA non-binding AFP, coated on a solid phase, reacted with 18H4 but reactivity with the LCA-binding species was inhibited by 60% following pretreatment of the AFP with LCA. The lectin was a very poor inhibitor of binding of 18H4 to the AFP which did not interact with LCA. In an alternative binding assay, a polyclonal anti-AFP coated solid phase was reacted with P-galactosidase-labelled 18H4. Pre-treatment with LCA of the LCA-reactive AFP gave 56% inhibition of binding of conjugated 18H4 while little inhibition was achieved with the LCA-nonreactive AFP component. These findings show that the epitope recognised by 18H4 is distinct from the glycan sequence that is reactive with LCA. However, the LCA-binding oligosaccharides occur in close proximity to the 18H4 epitope in the native AFP.

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“…Currently, most of the reported methods for the protein-based biomarkers detection have employed colorimetric enzyme immunoassays, 13 electrochemical immunoassays, 14 radioimmunoassays, 15 or fluoroimmunoassays. 16 However, these assays have technical limitations, including sample preparation complexity and specific detection instrumentation requirements.…”

Section: Introductionmentioning

confidence: 99%

Direct detection of cancer biomarkers in blood using a “place n play” modular polydimethylsiloxane pump

Zhang

Liao

et al. 2013

Biomicrofluidics

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Cancer biomarkers have significant potential as reliable tools for the early detection of the disease and for monitoring its recurrence. However, most current methods for biomarker detection have technical difficulties (such as sample preparation and specific detector requirements) which limit their application in point of care diagnostics. We developed an extremely simple, power-free microfluidic system for direct detection of cancer biomarkers in microliter volumes of whole blood. CEA and CYFRA21-1 were chosen as model cancer biomarkers. The system automatically extracted blood plasma from less than 3 ll of whole blood and performed a multiplex sample-to-answer assay (nano-ELISA (enzyme-linked immunosorbent assay) technique) without the use of external power or extra components. By taking advantage of the nano-ELISA technique, this microfluidic system detected CEA at a concentration of 50 pg/ml and CYFRA21-1 at a concentration of 60 pg/ml within 60 min. The combination of PnP polydimethylsiloxane (PDMS) pump and nano-ELISA technique in a single microchip system shows great promise for the detection of cancer biomarkers in a drop of blood. V C 2013 AIP Publishing LLC. [http://dx

show abstract

A monoclonal antibody-enzyme immunoassay for serum carcinoembryonic antigen with increased specificity for carcinomas.

Cited by 49 publications

References 15 publications

Investigation of nonspecific cross-reacting antigen 2 as a prognostic biomarker in bone marrow plasma from colorectal cancer patients

Investigation of nonspecific cross-reacting antigen 2 as a prognostic biomarker in bone marrow plasma from colorectal cancer patients

Close topographical relationship in alpha foetoprotein (AFP) between a lens culinaris binding glycan and the epitope recognized by AFP-reactive monoclonal antibody, 18H4

Direct detection of cancer biomarkers in blood using a “place n play” modular polydimethylsiloxane pump

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