A monoclonal antibody-based sensitive enzyme-linked immunosorbent assay (ELISA) for the analysis of the organophosphorous pesticides chlorpyrifos-methyl in real samples

Qian, Guoliang; Wang, Limin; Wu, Yunru; Zhang, Qi; Sun, Qinwei; Liu, Yang; Liu, Fengquan

doi:10.1016/j.foodchem.2009.03.097

Cited by 135 publications

(75 citation statements)

References 20 publications

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“…Trisodium citrate and methanol are analytical grade. Anti-chlorpyrifos-methyl mAb cell line (3G7/F5) and hapten-BSA (H1-BSA) were prepared and stored in our laboratory (Qian et al, 2009). Rabbit anti-mouse IgG was obtained from Henan Provincial Key Laboratory for Animal Immunology (Henan Academy of Agriculture Sciences, China).…”

Section: Reagents and Equipmentsmentioning

confidence: 99%

“…The preparation of H1 (chlorpyrifos-methyl hapten) and H1-BSA conjugate was described in a previous study (Qian et al, 2009). The anti-chlorpyrifos-methyl mAb cell line (3G7/F5) was efficiently cultivated by CELLine CL 350 according to the manufacturer's protocol.…”

Section: Preparation Of Antigen and Antibodymentioning

confidence: 99%

“…H1-BSA (Qian et al, 2009) and rabbit anti-mouse IgG were dispensed on the NC membrane by using XYZ-3000 dispensing platform. H1-BSA was dispensed around the bottom of the NC membrane, used as the test line (T line), and rabbit anti-mouse IgG was dispensed at the upper position of the NC membrane, used as the control line (C line).…”

Section: Immobilization Of Reagentsmentioning

confidence: 99%

“…In a previous work, a sensitive monoclonal antibody (3G7/F5)-based ELISA for determination chlorpyrifos-methyl in real samples has been successfully developed (Qian et al, 2009). In this study, we developed a rapid (8-10 min) and sensitive colloidal gold immunochromatographic assay, resulting in realizing qualitative and semi-quantitative detection for chlorpyrifos-methyl in water samples.…”

Section: Introductionmentioning

confidence: 99%

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Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples

Hua

Qian

Yang

et al. 2010

Biosensors and Bioelectronics

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Section: Reagents and Equipmentsmentioning

confidence: 99%

Section: Preparation Of Antigen and Antibodymentioning

confidence: 99%

Section: Immobilization Of Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples

Hua

Qian

Yang

et al. 2010

Biosensors and Bioelectronics

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“…Immunoassay has been an alternative to the instrument and microbiological methods for accurate measurements of antibiotic residues in complex matrices, because it is highly sensitive and specific, can be conducted on a large scale, is of low cost, and is rapid and simple to conduct. Unlike the instrument methods, immunoassays do not require sample pre-concentration and extraction (Abuknesha and Luk, 2005), so they can be used extensively in detecting trace amounts of chemicals such as antibiotics and pesticides (Aga et al, 2003;Abuknesha and Luk, 2005;Jin et al, 2008;Qian et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine

Shao-en

Zhou

2010

J. Zhejiang Univ. Sci. B

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Abstract:A protein conjugate of streptomycin (streptomycin-bovine serum albumin (BSA) conjugate) was prepared and used as immunogen to produce monoclonal antibodies (MAb). One hybridoma secreting anti-streptomycin MAb was obtained and then used to produce MAb. The MAb named 13H5 showed the 50% maximal inhibitory concentration (IC 50 ) value of 4.65 ng/ml and the IC 20 value of 0.21 ng/ml in phosphate buffered saline (PBS). At optimum conditions, an indirect competitive enzyme-linked immunosorbent assay (ELISA) and a colloidal gold-based immunochromatographic assay (CGIA) were developed and applied to detect streptomycin residues in milk and swine urine samples. The developed ELISA showed that the minimum detection limit was 2.0 and 1.9 ng/ml for milk and swine urine samples, respectively, without obvious cross-reactivity to other tested antibiotics except dihydrostreptomycin which gave a 118.32% cross reaction value. Milk and swine urine samples spiked with streptomycin at 10, 50, 100 and 200 ng/ml were analyzed by the established ELISA. The mean recovery of streptomycin was from 81.9% to 105.5% and from 84.3% to 92.2% for milk and swine urine, respectively. The optimized CGIA showed that the minimum detection limit was 20.0 ng/ml for milk and swine urine samples. The results of spiked analysis and specific analysis demonstrate that the CGIA could be applicable for screening milk and swine urine samples for the presence of streptomycin residues on-site. The established ELISA and CGIA allow the rapid, low-cost, and sensitive determination of streptomycin residues in food samples.

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Engineered Nanoenzymes with Multifunctional Properties for Next‐Generation Biological and Environmental Applications

Mishra

Lee

Kumar

et al. 2021

Adv Funct Materials

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As a powerful tool, nanoenzyme electrocatalyst broadens the ways to explore bioinspired solutions to the world's energy and environmental concerns. Efforts of fashioning novel nanoenzymes for effective electrode functionalization is generating innovative viable catalysts with high catalytic activity, low cost, high stability and versatility, and ease of production. High chemo‐selectivity and broad functional group tolerance of nanoenzyme with an intrinsic enzyme like activity make them an excellent environmental tool. The catalytic activities and kinetics of nanoenzymes that benefit the development of nanoenzyme‐based energy and environmental technologies by effectual electrode functionalization are discussed in this article. Further, a deep‐insight on recent developments in the state‐of‐art of nanoenzymes either in terms of electrocatalytic redox reactions (viz. oxygen evolution reaction, oxygen reduction reaction, nitrogen reduction reaction and hydrogen evolution reaction) or environmental remediation /treatment of wastewater/or monitoring of a variety of pollutants. The complex interdependence of the physicochemical properties and catalytic characteristics of nanoenzymes are discussed along with the exciting opportunities presented by nanomaterial‐based core structures adorned with nanoparticle active‐sites shell for enhanced catalytic processes. Thus, such modular architecture with multi‐enzymatic potential introduces an immense scope of making its economical scale‐up for multielectron‐fuel or product recovery and multi‐pollutant or pesticide remediation as reality.

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A monoclonal antibody-based sensitive enzyme-linked immunosorbent assay (ELISA) for the analysis of the organophosphorous pesticides chlorpyrifos-methyl in real samples

Cited by 135 publications

References 20 publications

Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples

Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples

Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine

Engineered Nanoenzymes with Multifunctional Properties for Next‐Generation Biological and Environmental Applications

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