Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine

Wu, Jiajing; Shao-en, Zhang; Zhou, Xueping

doi:10.1631/jzus.b0900215

Cited by 41 publications

(32 citation statements)

References 29 publications

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“…To date, different immunochromatographic assays of STR were described only in three papers [23][24][25]. However, the approach described in [23] is based on the use of the alkaline phosphatase enzyme as the label, and, correspondingly, the test strip is accomplished by a developing solution and the assay includes an additional step of the substrate transformation.…”

Section: Introductionmentioning

confidence: 99%

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Pretreatment-free immunochromatographic assay for the detection of streptomycin and its application to the control of milk and dairy products

Byzova

Zvereva

Zherdev

et al. 2011

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 99%

“…The detection limit obtained in [24] is 160 ng mL −1 for STR and 190 ng mL −1 for dihydrostreptomycin in raw milk. The system described in [25] allows the detection down to 20 ng mL −1 of STR in milk and swine urine. The duration of both assays is 10 min.…”

Section: Introductionmentioning

confidence: 99%

Pretreatment-free immunochromatographic assay for the detection of streptomycin and its application to the control of milk and dairy products

Byzova

Zvereva

Zherdev

et al. 2011

Analytica Chimica Acta

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“…The comparable level of cross-reactivity to dihydro-SM was described in polyclonal antibodies to conjugates of SM with albumin synthesized by different methods: for the synthesis through methylamino group with cyanuric chloride -75% (11), for adipic acid dihydrazide -80% (10) and 103% (7), in the case of carboxymethyloxime binding for aldehyde group -85,7% (3), 118% (12), and 149% (4). In this research, the derived antibodies expressed unusually high recognition of dihydro-SM possibly due to peculiarities of their hapten orientation relative to BSA in the immunogen, which resulted from specific conditions of conjugation with diglycidyl ether of 1,4-butanediol.…”

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confidence: 75%

“…In modern practice, the control of SM residues in animal products is often based on enzyme-linked immunosorbent assay (ELISA). This method was implemented in several countries in the form of test systems of various types including biosensor technology (3)(4)(5)(6)(7)(8)(9)(10)(11)(12). Russian researchers also have proposed a test system based on direct ELISA and the evaluation test using immunochromatography for the control of SM in milk and dairy products (13,14).…”

mentioning

confidence: 99%

“…According to the scientific literature, there are several ways to synthesize immunogens necessary for production of antibodies to SM, and each of them has become the basis of test systems for the control of livestock products. In 1993, it was reported about obtaining the antibodies to protein conjugates of SM modified by carboxymethyloxime for aldehyde group (3,4,8,12,13). Later, this was performed using the reaction of SM with glutaraldehyde and carbodiimide condensation (9), binding of methylamino group with cyanuric chloride (11,14), and modification of SM by adipic acid dihydrazide (6,7,10).…”

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Methods for Sanitary Surveillance of Livestock Production. Vii. Enzymoimmunoassay of Streptomycin

Burkin¹,

Galvidis²,

Кононенко³

2012

S-h. biol.

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S u m m a r yThe polyclonal rabbit antibodies against streptomycin conjugated with bovine serum albumin in reaction with diglycidyl ether of 1,4-butanediol presents high specificity to streptomycin and its similar structural analog -dihydrostreptomycin. In the conditions of indirect competitive enzymoimmunoassay with immobilized antigens, heterologous to immunogene on protein carrier and methods of synthesis, the limit of streptomycin detection is 0.1 ng/ml. The authors consider the use of developed immunoenzyme test-systems for control of antibiotic contamination in milk and eggs with the limit of detection of 10 mkg/kg and in meat with the limit of 20 mkg/kg. Keywords: streptomycin, milk, eggs, meat, immunoassay.Streptomycin (SM) is aminoglycoside antibiotic widely used for many years in our country and abroad for the treatment of acute infectious diseases of animals, despite its dangerous side effects such as allergic reactions, disorders of neuromuscular conduction, and ototoxicity (1 2). In modern practice, the control of SM residues in animal products is often based on enzyme-linked immunosorbent assay (ELISA). This method was implemented in several countries in the form of test systems of various types including biosensor technology (3-12). Russian researchers also have proposed a test system based on direct ELISA and the evaluation test using immunochromatography for the control of SM in milk and dairy products (13,14).The purpose of this work was obtaining specific immunoreagents based on streptomycin, optimization of conditions of the indirect competitive solid-phase immunoassay, and assessing the applicability of this test system for the control of SM residues in milk, eggs and meat.Technique. The experiment was performed using the following chemicals: streptomycin sulfate, diglycidyl ether of 1,4-butanediol, adipic acid dihydrazide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI), human transferrin (HT) ("Sigma", USA ), sodium borohydride ("Serva", Germany), dihydro-streptomycin (G.F. Gauze Research Institute of New Antibiotics, Moscow), dimethylformamide ("Fluka", Germany), bovine serum albumin (BSA), gelatin (Gel) and antispecies enzyme conjugate prepared according to the description (15) from horseradish peroxidase (EC 1.11.1.7) and donkey antiserum to rabbit immunoglobulin, sodium periodate of domestic production. ELISA was conducted on high-binding polystyrene plates ("Costar", USA) using a photometer Dynatech MR 5000 ("Dynatech", Germany).The reaction with diglycidyl ether of 1,4-butanediol for the synthesis of conjugates of SM with BSA and Gel -BSA-SM(100)e, Gel-SM(10)e, Gel-SM(30)e, and Gel-SM(100)e: 6,8 mg SM sulfate (10 umol) in 1 ml 1% NaHCO 3 was added with 14 ul (10 umol) diglycidyl ether of 1,4-butanediol (10% solution in dimethylformamide) and stirred at room temperature for 24 hours. Then, 4 mg BSA (0,06 umol) in 0,5 ml of 0,05 M carbonate-bicarbonate buffer (CBB, pH 9,5) was added with 600 ul of the abovementioned mixture (100-fold molar excess of hapten), and thr...

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Fabrication of molecularly imprinted cotton fibers for quantification of streptomycin in honey samples

Zengi̇n

Badak

Bilici

et al. 2023

J of Applied Polymer Sci

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Streptomycin is a type of aminoglycoside used in treatment of several infections and with serious side effects in humans and animals, such as ototoxicity and nephrotoxicity. In the present study, we focused on the preparation of streptomycin‐imprinted cotton fiber for rapid, sensitive and selective quantification of streptomycin in honey samples. The imprinted cotton fibers were characterized by attenuated total reflectance‐Fourier transform spectroscopy, scanning electron microscopy, and x‐ray photoelectron spectroscopy. In addition, the imprinted cotton fibers showed high adsorption capacity (95.4 mg/g) with an imprinting factor of 3.69 and had satisfactory regeneration ability of up to 10 regenerations without significant change in adsorption capacity. The imprinted cotton fibers showed good linearity with a correlation coefficient of 0.9989 and limit of detection was determined to be 0.29 ng/mL for streptomycin in honey samples. The proposed method showed acceptable recovery ranges (98.0%–100.3%) with lower inter‐day and intra‐day precisions. Moreover, the performance of the proposed method was compared to the ELISA method. The results showed that the present method is as sensitive and selective as ELISA. It is concluded that the imprinted cotton fibers could be a potential alternative to antibiotic quantification based on traditional immunoassays or chromatographic techniques.

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Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine

Cited by 41 publications

References 29 publications

Pretreatment-free immunochromatographic assay for the detection of streptomycin and its application to the control of milk and dairy products

Pretreatment-free immunochromatographic assay for the detection of streptomycin and its application to the control of milk and dairy products

Methods for Sanitary Surveillance of Livestock Production. Vii. Enzymoimmunoassay of Streptomycin

Fabrication of molecularly imprinted cotton fibers for quantification of streptomycin in honey samples

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