A monoclonal antibody against Meningococcus group B polysaccharides used to immunocapture and quantify polysialylated NCAM in tissues and biological fluids

Dubois, C.; Okandze, Antoine; Figarella‐Branger, Dominique; Rampini, C; Rougon, Geneviève

doi:10.1016/0022-1759(94)00336-u

Cited by 16 publications

(6 citation statements)

References 32 publications

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“…The PSA‐NCAM antibody was raised against the polysaccharide of Meningococcus B, a highly polymeric form of α2‐8 sialic acid and recognizing the polysialic acid residues of a minimum length of 12 residues. The specificity of this antibody was determined to only recognize an 180 kDa band by Western blot (Rougon et al, 1986; Szele et al, 1994; Dubois et al, 1995). Preabsorption of the antibody with colominic acid completely abolished the fluorescent signal in cell lines (Rougon et al, 1986).…”

Section: Methodsmentioning

confidence: 99%

Transplantation of multipotent cells extracted from adult skeletal muscles into the subventricular zone of adult rats

et al. 2005

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Stem cells isolated from adult tissues may be useful for autologous cell therapy in the nervous system. In the present study we tested the ability of multipotent stem cells isolated from adult muscle to survive and respond to migratory and differentiating cues when transplanted into the adult subventricular zone (SVZ). Prior to transplantation the cells were grown as spheres that expressed doublecortin, nestin, and betaIII-tubulin, as well as the mRNAs for the receptor EphA4 and the ligands ephrin B1, ephrin B2, but not ephrin B3. Four weeks after transplantation into the anterior part of the SVZ in adult rats, surviving cells were observed along the ventricular wall, in the SVZ, and in the posterior rostral migratory stream (RMS). None of these cells stained for betaIII-tubulin or doublecortin, which are molecules expressed by migrating neuroblasts, and none were present in the more rostral regions of the RMS or the olfactory bulb. However, most surviving transplanted cells were integrated into the wall of the lateral ventricle and expressed vimentin, a marker also expressed by ependymocytes. No tumors were observed 4 weeks posttransplantation. Our results suggest that multipotent stem cells isolated from adult muscle, which can be easily and safely isolated from patients and rapidly expanded ex vivo, may provide autologous vectors for the local delivery of secreted factors to the ventricles or nearby regions.

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Section: Methodsmentioning

confidence: 99%

Transplantation of multipotent cells extracted from adult skeletal muscles into the subventricular zone of adult rats

et al. 2005

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“…The mice were killed as described above. In six injected and three control mice, ascites were collected and stored at 7808C; PSA levels were measured by using an ELISA previously described (Dubois et al, 1995). Primary tumors, lungs, and livers were processed as above.…”

Section: Endo-n Injection and Tumor Monitoringmentioning

confidence: 99%

A nude mice model of human rhabdomyosarcoma lung metastases for evaluating the role of polysialic acids in the metastatic process

Daniel

Durbec²,

Gautherot³

et al. 2001

Oncogene

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PSA is an oncodevelopmental antigen usually expressed in human tumors with high metastatic potential. Here we set up a metastatic model in nude mice by using TE671 cells, which strongly express PSA-NCAM. We observed the formation of lung metastases when TE671 cells were injected intravenously, intramuscularly, and intraperitoneously, but not subcutaneously. Intraperitoneous injections also induced peritoneal carcinosis, ascites, and liver metastases. To evaluate the putative role of PSA in the metastatic process we used a speci®c cleavage of PSA on NCAM by endoneuraminidase-N on intraperitoneous primary tumors. Mice with primary intramuscular tumors were taken as control. Repeated injections of endoneuraminidase-N led to a decrease in PSA expression in primary intraperitoneous nodules and ascites but not in intramuscular primary tumors. Endoneuraminidase-N also increased the delay in ascitic formation and decreased the number of lung or liver metastases in the case of intraperitoneous tumors but not in the case of intramuscular tumors. When metastases occurred in endoneuraminidase-N injected animals, they strongly expressed PSA-NCAM. Therefore, we established a relationship between PSA expression on the surface of primary tumor cells and the metastatic process. Oncogene (2001) 20, 997 ± 1004.

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“…Indirect ELISA has often been used for polySia quantification. This utilises an anti-polySia antibody as the primary (capture) antibody to detect polySia in cells and tissues, coupled with a secondary antibody (detection and quantitation antibody) (Dubois, Okandze, Figarella-Branger, Rampini, & Rougon, 1995;Sato et al, 1995;Hildebrandt et al, 1998;Poongodi et al, 2002;Seidenfaden, Krauter, Schertzinger, Gerardy-Schahn, & Hildebrandt, 2003;Amoureux et al, 2010). ELISA is generally a reproducible and reliable method providing high specificity and sensitivity.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

“…Sandwich ELISAs, which employ two antibodies to recognise two different epitopes of target polysialylated proteins can also be used. (Dubois et al, 1995;Amoureux et al, 2010;Piras et al, 2015). For example, as little as 600 pg of polySia-NCAM per microgram of brain extract was determined using a Sandwich ELISA employing an anti-Meningococcus group B antibody as the capture antibody (Dubois et al, 1995).…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Recent advances in the analysis of polysialic acid from complex biological systems

Guo

Elkashef

Loadman

et al. 2019

Carbohydrate Polymers

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A monoclonal antibody against Meningococcus group B polysaccharides used to immunocapture and quantify polysialylated NCAM in tissues and biological fluids

Cited by 16 publications

References 32 publications

Transplantation of multipotent cells extracted from adult skeletal muscles into the subventricular zone of adult rats

Transplantation of multipotent cells extracted from adult skeletal muscles into the subventricular zone of adult rats

A nude mice model of human rhabdomyosarcoma lung metastases for evaluating the role of polysialic acids in the metastatic process

Recent advances in the analysis of polysialic acid from complex biological systems

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