A modified radioimmunoassay for antibodies against <i>Brucella abortus</i>

Chappel, R.J.; Hayes, J S; Brain, G. J.; McNaught, D. J.

doi:10.1017/s0022172400069837

Cited by 9 publications

(3 citation statements)

References 9 publications

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“…In spite of the shortcomings, the complement fixation test has been and is a valuable asset as a confirmatory test in control/eradication programs. There are a number of variations of the test, including the indirect haemolysis test [72][73][74][75][76][77][78][79][80], which are not widely used.…”

Section: Complement Fixation Testsmentioning

confidence: 99%

Diagnosis of Brucellosis

Poester¹,

Nielsen²,

Samartino³

et al. 2010

TOVSJ

107

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Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals. Abortion, placentitis, epididymitis, and orchitis are the most common clinical manifestations in animals. In humans, brucellosis is a debilitating and chronic disease, which may affect a variety of organs. Clinical diagnosis of brucellosis is not easily achieved. Laboratory testing is therefore very important for a correct identification of the disease in humans and for the detection and confirmation in animals. Definitive diagnosis is normally done by isolation and identification of the causative agent. While definitive, isolation is time-consuming, must be performed by highly skilled personnel, and it is hazardous. For these reasons, serological tests are normally preferred. Brucellosis serology have advanced considerably in the last decades with very sensitive and specific new tests available. Modern genetic characterization of Brucellae using molecular DNA technology have been developed. Several PCR-based assays have been proposed, from the rapid recognition of genus to differential identification of species and strains. This review describes bacteriological, serological, and molecular methods used for the diagnosis of human and animal brucellosis.

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Section: Complement Fixation Testsmentioning

confidence: 99%

Diagnosis of Brucellosis

Poester¹,

Nielsen²,

Samartino³

et al. 2010

TOVSJ

107

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“…All sera were submitted to the Rose Bengal plate test (RBPT) (Anon. 1980), and RBPT-positive sera were tested by RIA (Chappel et al 1982a), the CFT using 3-2 warm fixation (Anon. 1977) and the IHLT (Plackett, Cottew & Best, 1976).…”

Section: Methodsmentioning

confidence: 99%

“…False negative serological reactions contribute to the existence of problem herds which resist eradication (Cordes & Carter, 1979). The brucellosis radioimmunoassay (RIA) (Chappel et al 1982a) is a sensitive test designed to reduce the number of false negative serological diagnoses.…”

Section: Introductionmentioning

confidence: 99%

Comparison of radioimmunoassay with the complement fixation test and the indirect haemolysis test in the field diagnosis of bovine brucellosis

Chappel

Hayes

1983

J. Hyg.

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Sera were collected from female cattle in 118 commercial herds being subjected to a programme to eradicate brucellosis by test and slaughter, in an area in which vaccination of heifer calves with Brucella abortus strain 19 was compulsory. Of 4583 sera positive by the Rose Bengal plate test, the brucellosis radioimmunoassay was positive for 1524, the complement fixation test for 1363 and the indirect haemolysis test for 1141. These figures, and supporting evidence from the eradication programme, suggest that the radioimmunoassay may be a useful supplementary test in problem herds.

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