Solid-phase radioimmunoassay for the detection of immunoglobulins against bovine

Lawman, M. J. P.; Thurmond, Mark; Reis, Kathleen J.; Gauntlett, D. R.; Boyle, M. D.P.

doi:10.1016/0165-2427(84)90055-2

Cited by 20 publications

(6 citation statements)

References 12 publications

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“…In experimentally infected cattle, RIA was most sensitive in cattle that had been vaccinated and gave fewer false-negative reactions than other serological tests (Hayes and Chappel 1982). A solid-phase RIA, utilizing 96-well microtiter plates coated with antigen and 125 I-labeled staphylococcal protein A for detection, was considered sensitive and able to discriminate between false positives and false negatives (Lawman et al 1984). Antibody detection was 16 to 32 times more sensitive than CFT and 4- to 64-fold more sensitive than the standard tube test titer.…”

Section: Introductionmentioning

confidence: 99%

The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

Viljoen

Luckins

2012

Trop Anim Health Prod

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Nuclear and nuclear-related technologies have played an important role in animal health, particularly in relation to disease diagnosis and characterization of pathogenic organisms. This review focuses primarily on how and where nuclear technologies, both non-isotopic and isotopic methods, have made their impact in the past and where it might be expected they could have an impact in the future. The review outlines the extensive use of radiation attenuation in attempts to create vaccines for a multiplicity of pathogenic organisms and how the technology is being re-examined in the light of recent advances in irradiation techniques and cryopreservation/lyophilization that might obviate some of the problems of maintenance of viable, attenuate vaccines and their transport and use in the field. This approach could be used for a number of parasitic diseases where vaccination has been problematic and where investigations into the development of molecular vaccines have still failed to deliver satisfactory candidates for generating protective immune responses. Irradiation of antigens or serum samples also has its uses in diagnosis, especially when the samples need to be transported across international boundaries, or when handling the pathogens in question when carrying out a test presents serious health hazards to laboratory personnel. The present-day extensive use of enzyme immunoassays and molecular methods (e.g., polymerase chain reaction) for diagnosis and characterization of animal pathogens has its origins in the use of isotope-labeled antigens and antibodies. These isotopic techniques that included the use of 75Se, 32P, 125I, and 35S isotopes enabled a level of sensitivity and specificity that was hitherto unrealized, and it is prescient to remind ourselves of just how successful these technologies were, in spite of their infrequent use nowadays. Finally, the review looks at the potential for stable isotope analysis for a variety of applications—in the tracking of animal migrations, where the migrant are potential carriers of transboundary animal diseases, and where it would be useful to determine the origins of the carrier, e.g., Highly Pathogenic Avian Influenza and its dissemination by wild water fowl. Other applications could be in monitoring sequestered microbial culture (e.g., rinderpest virus) where in the case of accidental or deliberate release of infective culture it would be possible to identify the laboratory from which the isolate originated.

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Section: Introductionmentioning

confidence: 99%

The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

Viljoen

Luckins

2012

Trop Anim Health Prod

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“…Most methods currently used for diagnosis of Brucellosis are based on the detection of specific antibodies in the serum or in other body fluids of suspicious animals (BYRD et al, 1978;CHAPPEL et al, 1982;LAWMAN et al, 1984). These methods are hampered by extensive cross-reactions of the antibodies with other gram-negative bacteria (CIOGLIA, 1950;FEELEY, 1969;HURVELL, 1972) which make the serodiagnostic tests erratic and often inconclusive.…”

Section: Discussionmentioning

confidence: 99%

Characterization of monoclonal antibodies against Brucella melitensis

Greiser‐Wilke

Moennig

Thon

et al. 1985

Zentralblatt für Veterinärmedizin Reihe B

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Summary Eight specific monoclonal antibodies were isolated from fusions of myeloma cells with spleen‐cells from mice immunized with Brucella melitensis 16 M. An Enzyme‐linked immunosorbent assay was used to screen the hybridoma supernatants and to determine the cross‐reactivity of the monoclonal antibodies with other bacteria. Five monoclonal antibodies reacted with smooth and rough Brucella strains and with all gram‐negative bacteria tested. One monoclonal antibody reacted with smooth Brucella strains and with Yersinia enterocolitica 0:9, and two monoclonal antibodies were specific for smooth Brucella strains. The latter two antibodies belonged to the antibody subclasses IgG 2 a (BM/38) and IgG 1 (BM/40). Both cultures were grown in serum‐free medium and were subsequently concentrated by AMICON ultrafiltration. The protein concentrations ranged between 1 and 3.5 mg/ml, the main contamination being residues of bovine serum albumin (40—60%). Samples of the concentrated antibodies were used for peroxidase‐labelling and were found to have high titres as determined by ELISA. A double antibody sandwich ELISA with both monoclonal antibodies was devised which was highly specific for Brucella melitensis 16 M. The applicability of such a test for diagnostic purposes is discussed. Zusammenfassung Brucella melitensis 16 M wurde zur Immunisierung von Mäusen verwendet. Durch die Fusion von Lymphozyten immuner Tiere mit Myelomzellen wurden acht Zellinien etabliert, die spezifische Antikörper produzieren. Ein „Enzyme‐linked Immunsorbent Assay” (ELISA) wurde zum Nachweis der Antikörper in den Hybridoma Überständen und zum Nachweis von Kreuzreaktionen mit anderen Bakterien verwendet. Fünf monoklonale Antikörper reagierten mit glatten und rauhen Brucella sp. und mit allen untersuchten gramnegativen Bakterien. Ein monoklonaler Antikörper reagierte mit glatten Brucella sp. und mit Yersinia enterocolitica 0 : 9, während zwei monoklonale Antikörper spezifisch für glatte Brucella‐Stämme waren. Die beiden letzteren gehörten zur IgG Subklasse 2 a (BM/38) bzw. zur Subklasse 1 (BM/40). Beide Antikörper wurden in serumfreiem Kulturmedium produziert und durch Amicon Ultrafiltration konzentriert. Die Proteinkonzentrationen lagen zwischen 1 und 3.5 mg/ml. Die Hauptkontamination bestand aus Resten von fetalem Kälberserum (40—60%). Ein Teil der konzentrierten Antikörper wurde mit Peroxidase markiert. Diese Antikörper zeigten einen hohen Titer im ELISA. Mit markiertem BM/40 und nicht‐markiertem BM/38 wurde ein ELISA zum Antigennachweis etabliert. Der Test war spezifisch für Brucella melitensis 16 M. Die mögliche Anwendung einer solchen Nachweismethode für diagnostische Zwecke wird erörtert. Résumé Caractérisation d'anticorps monoclonaux contre Brucella melitensis Brucella melitensis 16 M a été utilisée pour immuniser des souris. Par la fusion de lymphocytes des animaux immunisés avec des cellules de myélome, on a établi huit lignées cellulaires qui produisaient des anticorps. Un test ELISA (Enzyme‐linked‐Immunosorbent Assay) fut utilisé pour la m...

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“…Complement Fixation test (CFT) are among the most widely adopted procedures for the sero-diagnosis of brucellosis. There are certain limitations associated with these tests such as anticomplementary activity, prozone phenomenon and inability to detect IgGz antibody by CFT ( PLACKETT et al, 1976;SUTHERLAND, 1980); low sensitivity of SAT and RBPT in culture proven cases (ALTON et al, 1975 b;CARGILL et al, 1985) and over sensitiveness of (ALTON et al, 1975 a) but some of these have been largely overcome with the development of solid phase enzyme or radio-immuno assays (STEMSHORN et al, 1980;CARGILL et al, 1985;SUTHERLAND and HOLLANDER, 1986;LAWMAN et al, 1984;LAWMAN et al, 1986). These solid phase immunoassays are most commonly performed using polystyrene plate as the adsorbent matrix (VOLLER et al, 1976).…”

Section: Serum Agglutination Test (Sat) Rose Bengal Plate Agglutinatmentioning

confidence: 99%

Comparison of Dot Enzyme‐linked Immunosorbent Assay (Dot‐ELISA) with other Serological Tests for the Detection of Brucella Antibodies in Bovine Sera

Chand

Sadana

Batra

et al. 1989

Journal of Veterinary Medicine, Series B

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A dot Enzyme-linked Immunosorbent Assay (dot-ELISA), using whole cell Erucelka abortus antigen dotted on the nitrocellulose membrane bound to a plastic strip (dipstick) was employed for the detection of Erucella antibodies in bovine sera. The results were compared with that of serum agglutination (SAT), Rose Bengal plate agglutination (RBPT) and Complement Fixation test (CFT). All the four tests gave negative reaction in 127 sera obtained from a brucellosis free herd. Testing of 549 sera from a chronically infected herd revealed 57 positive and 447 negative animals in all the four assays. Of the remaining 45 sera, 34 were positive in dot-ELISA. Six of these cases were independently detected by dot-ELISA while 28 showed positive reactions in combination with other tests. When serum samples from 158 aborted cases were subjected to dot-ELISA, 79 were found positive. Of these dot-ELISA positive cases, 71 gave positive reaction in SAT, 72 in RBPT and 78 in CFT. E. abortus biotype 3 was isolated from 34 of the 98 aborted fetuses examined.

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Solid-phase radioimmunoassay for the detection of immunoglobulins against bovine

Cited by 20 publications

References 12 publications

The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

Characterization of monoclonal antibodies against Brucella melitensis

Comparison of Dot Enzyme‐linked Immunosorbent Assay (Dot‐ELISA) with other Serological Tests for the Detection of Brucella Antibodies in Bovine Sera

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