Diagnosis of Brucellosis

Poester, Fernando Padilla; Nielsen, Klaus; Samartino, Luis; Wang, Yu

doi:10.2174/1874318801004010046

Cited by 106 publications

(48 citation statements)

References 184 publications

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“…abortion and strong seropositive results finally led to the diagnosis of brucellosis. Serological diagnosis from freshly aborted animals may fail because antibody titers against B. abortus rise only 1–2 weeks after infection [16], however circulating Brucella DNA may be detected with molecular techniques. These facts can explain the absences of antibody titres in some animals.…”

Section: Discussionmentioning

confidence: 99%

Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR

et al. 2015

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BackgroundBrucellosis is a major zoonoses affects wide range of domesticated as well as wild animals. Despite the eradication program of brucellosis in Egypt, the disease is still endemic among cattle, buffaloes, sheep, goats, and camels.ResultsIn the present study, abortion occurred naturally among 25 animals (10 cows, 5 buffaloes, 9 Egyptian Baladi goats and 1 ewe) shared the same pasture were investigated by real-time polymerase chain reaction (RT-PCR). DNA of Brucella (B.) abortus was detected in serum of goats and sheep which has aborted recently by species-specific RT-PCR. The results suggest cross-species infection of B. abortus from cattle to non-preferred hosts raised in close contact.ConclusionThis article will renew our knowledge about the Brucella agent causing abortion in small ruminants in Egypt. Information provided in this study is important for surveillance program, because eradication programs and vaccination strategies may have to be adapted accordingly.

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Section: Discussionmentioning

confidence: 99%

Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR

et al. 2015

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“…Milk samples were centrifuged to concentrate bacteria, at 6000–7000 g for 15 min in sealed tubes to avoid potential for aerosolization [7, 44]. A mixture of cream and deposit was streaked both on petri dishes with Farrell’s medium ( Brucella medium base, CM0169; antibiotic supplement, SR0083, Oxoid™) and with CITA medium (blood agar base number 2, CM0271, Oxoid™; and antibiotic supplements vancomycin, colistin, nystatin, nitrofurantoin, amphotericin B, Sigma™, as well as containing 5–10%, inactivated horse serum, SR0035, Oxoid™).…”

Section: Methodsmentioning

confidence: 99%

Risk factors of brucellosis seropositivity in Bactrian camels of Mongolia

Bayasgalan

Chultemdorj

Roth³

et al. 2018

BMC Vet Res

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BackgroundMore information on brucellosis epidemiology in Bactrian camels is needed due to their growing economic and livelihood importance for herders and renewed efforts in Mongolia to eliminate brucellosis through mass vaccination of ruminants excluding camels. Brucellosis prevalence in camels increased over the past two decades. Random multi-stage cluster surveys were done in the Eastern provinces of Dornod and Sukhbaatar in 2013 and 2014 and in the Southern & Western provinces of Dornogobi, Umnogobi and Khovd in 2014 and 2015. A total of 1822 camels, 1155 cattle, and 3023 small ruminant sera were collected and tested with the Rose Bengal Test. In addition, 195 vaginal swabs and 250 milk samples for bacteriological culture were taken from livestock with history of abortion.ResultsThe overall apparent seroprevalence in camels was 2.3% (95% confidence interval 1.6–3.3). The main risk factor for camel seropositivity was being in an Eastern province when compared to Southern & Western provinces (odds ratio 13.2, 95% CI 5.3–32.4). Camel seroprevalences were stable over the two consecutive survey years, despite introduction of ruminant vaccination: 5.7% (95% CI 3.1–10.2%) and 5.8% (3.3–10.1%) in Eastern provinces and 0.4% (0.2–1.2%) and 0.5% (0.1–2.0%) in Southern & Western provinces. We isolated Brucella abortus from camels and cattle. Camel seropositivity was associated to keeping cattle together with camels. Monitoring of vaccination campaigns showed that coverage in cattle was insufficient because animals could not be adequately restrained.ConclusionsThe present study reveals that brucellosis is present with important seroprevalence in Mongolian camels and was endemic in Eastern provinces. Camel herd seropositivity was most closely associated to infection in cattle.Longer term monitoring is needed to assess whether camel seroprevalance decreases with ongoing vaccination in Mongolia. This should be coupled with further confirmation on Brucella spp. isolates. To date, only Brucella abortus was isolated, but camels are also susceptible to Brucella melitensis. Clear verbal and written information on disease prevention in livestock and household members is important, particularly for remote camel herders who had only moderate knowledge on brucellosis epidemiology and preventive measures.Electronic supplementary materialThe online version of this article (10.1186/s12917-018-1664-0) contains supplementary material, which is available to authorized users.

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“…Serological tests are widely used to conduct several epidemiological studies and diagnostic purposes, but there is no perfect serological test [14, 15]. However, the diagnostic performance and discriminative ability of a test could be evaluated by comparing the sensitivity and specificity of several tests analytically [14, 16].…”

Section: Introductionmentioning

confidence: 99%

Bayesian Estimation of Sensitivity and Specificity of Rose Bengal, Complement Fixation, and Indirect ELISA Tests for the Diagnosis of Bovine Brucellosis in Ethiopia

Getachew¹,

Getachew²,

Sintayehu³

et al. 2016

Veterinary Medicine International

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Test evaluation in the absence of a gold standard test was conducted for the diagnosis and screening of bovine brucellosis using three commercially available tests including RBPT, CFT, and I-ELISA in National Animal Health Diagnostic and Investigation Center (NAHDIC) Ethiopia. A total of 278 sera samples from five dairy herds were collected and tested. Each serum sample was subjected to the three tests and the results obtained were recorded and the test outcomes were cross-classified to estimate the sensitivity and specificity of the tests using Bayesian model. Prior information generated on the sensitivity and specificity of bovine brucellosis from published data was used in the model. The three test-one population Bayesian model was modified and applied using WinBug software with the assumption that the dairy herds have similar management system and unknown disease status. The Bayesian posterior estimate for sensitivity was 89.6 (95% PI: 79.9–95.8), 96.8 (95% PI: 92.3–99.1), and 94 (95% PI: 87.8–97.5) and for specificity was 84.5 (95% PI: 68–94.98), 96.3 (95% PI: 91.7–98.8), and 88.5 (95% PI: 81–93.8) for RBT, I-ELISA, and CFT, respectively. In this study I-ELISA was found with the best sensitivity and specificity estimates 96.8 (95% PI: 92.3–99.1) and 96.3 (95% PI: 91.7–98.8), compared to both CFT and RBPT.

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Diagnosis of Brucellosis

Cited by 106 publications

References 184 publications

Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR

Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR

Risk factors of brucellosis seropositivity in Bactrian camels of Mongolia

Bayesian Estimation of Sensitivity and Specificity of Rose Bengal, Complement Fixation, and Indirect ELISA Tests for the Diagnosis of Bovine Brucellosis in Ethiopia

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