Bayesian Estimation of Sensitivity and Specificity of Rose Bengal, Complement Fixation, and Indirect ELISA Tests for the Diagnosis of Bovine Brucellosis in Ethiopia

Getachew, Tesfaye; Getachew, G.; Sintayehu, G; Getenet, M.; Fasil, A.

doi:10.1155/2016/8032753

Cited by 32 publications

(32 citation statements)

References 15 publications

(26 reference statements)

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“…Bayesian method provides a stable point and interval estimate without the necessity of large sample size [ 23 ]. The sensitivity of RBPT in the present study (58.3% (26.4–92.7%)) is similar to the finding of Sanogo et al [ 34 ] (54.9% (23.5–95.1)) and lower than the finding of Getachew et al [ 57 ] (89.6% (79.9–95.8)). The specificity of RBPT (92.1% (88.7–95.2) was fairly high and similar to the finding of Getachew et al [ 57 ] (84.5% (68–94.8)) and Sanogo et al [ 34 ] (97.7% (95.3–99.4)).…”

Section: Discussionsupporting

confidence: 89%

Seroprevalence and Associated Risk Factors of Brucellosis among Indigenous Cattle in the Adamawa and North Regions of Cameroon

Awah-Ndukum

Mouiche

Bayang

et al. 2018

Veterinary Medicine International

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A cross-sectional seroprevalence study was conducted on cattle in the North and Adamawa Regions of Cameroon to investigate the status of bovine brucellosis and identify potential risk factors. The diagnosis was carried out using the Rose Bengal Plate test (RBPT) and indirect ELISA (i-ELISA), while questionnaires were used to evaluate risk factors for bovine brucellosis in cattle. The Bayesian approach was used to evaluate the diagnostic tests' sensitivity and specificity. The overall individual level (n = 1031) and herd level (n = 82) seroprevalence were 5.4% (0.4–10.5) and 25.6% (16.2–35.0), respectively. Bayesian analysis revealed sensitivity of 58.3% (26.4–92.7) and 89.6% (80.4–99.4) and specificity of 92.1% (88.7–95.2) and 95.7% (91.1–99.7) for RBPT and i-ELISA, respectively. Management related factors such as region, locality, herd size, and knowledge of brucellosis and animal related factors such as sex and age were significantly associated with seropositivity of brucellosis. Zoonotic brucellosis is a neglected disease in Cameroon. The study highlights the need for control measures and the need to raise public awareness of the zoonotic occurrence and transmission of bovine brucellosis in the country. An integrated disease control strategy mimicking the one health approach involving medical personnel, veterinarians, related stakeholders, and affected communities cannot be overemphasized.

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Section: Discussionsupporting

confidence: 89%

Seroprevalence and Associated Risk Factors of Brucellosis among Indigenous Cattle in the Adamawa and North Regions of Cameroon

Awah-Ndukum

Mouiche

Bayang

et al. 2018

Veterinary Medicine International

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“…However, Cakan et al [51] showed ELISA test for brucellosis is more sensitive only when both IgG and IgM were used, though their titer alone didn't represent disease status. Awah-Ndukum et al [57] , Sanogo et al [58] , and Gatechew et al [59] reported sensitivity and specificity of ELISA IgG were 95.6% &97.1%, 96.1% &95%, and 96.8% &96.3%; respectively.…”

Section: Discussionmentioning

confidence: 98%

Human Brucellosis: Methods of Diagnosis and Risk Factors among Egyptian Patients admitted at Assiut Fever Hospital .

El-Moselhy

Soliman

El-Rahman

et al. 2020

Journal of Recent Advances in Medicine

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Background: Human brucellosis, a common zoonotic disease, is major public health problem in many countries worldwide including Egypt. Objectives: To define brucellosis patients' risk-factors and to assess diagnostic lab methods of brucellosis at Assiut Fever Hospital. Patients and Methods: The study recruited 98 patients with brucellosis and an equal number of controls. All participants were subjected to interview, clinical examination, and lab investigations. Results: Older age, males, rural residence, low socioeconomic status were significant risk-factors (OR=3.76, 2.04, 2.86, 2.72; respectively). Occupations had animals' contact were significant risk-factor (OR=4.7); the most risky were butchers/ slaughter workers (OR=8.0) and farmers/dairy workers (OR=3.59). Longer occupational exposure was risk-factor (OR=15.57). The main significant presenting symptoms were fever and musucloskeletal affections. The main significant signs were high temperature and hepato-and spleno-megaly. Standard agglutination test (SAT) titer 1/320 was the cutoff point for diagnosis and significantly lies in area under the ROC curve, sensitivity=96.4% and specificity=100.0%. Blood culture was positive in 58.2% of cases with no significant differences between SAT titer and blood culture positivity. ELISA IgM and IgG results were positive in 69.4% and 65.3% of the cases with no significant differences between SAT titer and IgM and IgG results. Conclusions: Human brucellosis has many preventable risk-factors; its diagnosis depends mainly on presence of riskfactors, clinically suspected, and SAT titer ≥1/320.

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“…The overall sero-prevalence of Brucella antibodies determined with RBPT and I-ELISA in Becho district, Oromia regional state were 3.39% and 1.04%, respectively. Since I-ELISA is the recommended diagnostic test for brucellosis with higher sensitivity and specificity (Getachew et al, 2016;Uzal et al, 1995), the overall seroprevalence of bovine brucellosis in the study area is 1.04%. This low sero-prevalence is in agreement with the earlier report of (Meles and Kibeb, 2018) with the same prevalence in the Chencha district, GamoGofa zone, South-Eastern Ethiopia, (Adugna et al 2013;Kang'ethe et al, 2007) In this study breed was supposed to be one of the risk factors, consequently sero-prevalence was found to be higher in cross-breed animals (3.13%) than local breeds (0.35%).…”

Section: Discussionmentioning

confidence: 99%

Sero-Prevalence of Bovine Brucellosis and its Associated Risk Factors in Becho District, South West Shewa, Oromia Regional State, Ethiopia

2019

ARC Journal of Animal and Veterinary Sciences

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A cross-sectional study was conducted in selected areas of Becho district, Oromia regional state from December, 2018 to May, 2019 to estimate the sero-prevalence of bovine brucellosis in the study area and identify the associated risk factors for the seropositivity. Simple random sampling method was used for sampling each herd and animal during serum collection, and risk factors accessed through interviewee of the animal owners. A total of 384 serum samples were collected from cattle of above 6 months of age and sera were initially screened with Rose Bengal Plate Test (RBPT) and those samples found positive by RBPT were further tested by Indirect Enzyme-linked Immuno Sorbent Assay (I-ELISA) for confirmation. Based on the result, 13(3.39%) were positive using RBPT and the overall sero-prevalence of bovine brucellosis at animal-level estimated to be 4(1.04%) based on I-ELISA result. At herds' level, an overall sero-prevalence of 5.48% was obtained. Pearson's Chi-square revealed that the presence of abortion history, retained fetal membrane and breed type were significantly associated with the seropositivity of bovine brucellosis (p<0.05) with Chi-square value of 38.2, 5.76 and 3.99 and P-value of 0.000, 0.016 and 0.046, respectively. The odds of having brucellosis increased by 12.4 times in cow with history of abortion compared to cow without the history, increased by 4.5 times in the animal suffered from history of retained placenta compared to cow with no history of retained placenta and 3.1 times in crossbreed than indigenous breeds. In conclusion, the study demonstrate that the prevalence of bovine brucellosis was low in Becho district. However, poor understanding of brucellosis among communities and a high level of risky practice might result in an increased transmission between animals and zoonoses in risky group personnels. Thus, awareness creation about impacts of the disease, zoonotic importance, risk factors and method of prevention were recommended.

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Bayesian Estimation of Sensitivity and Specificity of Rose Bengal, Complement Fixation, and Indirect ELISA Tests for the Diagnosis of Bovine Brucellosis in Ethiopia

Cited by 32 publications

References 15 publications

Seroprevalence and Associated Risk Factors of Brucellosis among Indigenous Cattle in the Adamawa and North Regions of Cameroon

Seroprevalence and Associated Risk Factors of Brucellosis among Indigenous Cattle in the Adamawa and North Regions of Cameroon

Human Brucellosis: Methods of Diagnosis and Risk Factors among Egyptian Patients admitted at Assiut Fever Hospital .

Sero-Prevalence of Bovine Brucellosis and its Associated Risk Factors in Becho District, South West Shewa, Oromia Regional State, Ethiopia

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