A modified plate assay for screening phosphate solubilizing microorganisms.

Gupta, Rani; Singal, Rekha; Shankar, Aparna; Kuhad, Ramesh Chander; Saxena, Rekha

doi:10.2323/jgam.40.255

Cited by 199 publications

(113 citation statements)

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“…The solubilisation zone varied from 0.0 to 8.0 mm on PVK, 1.0 to 8.6 mm on MPVK and 1.3 to 7.6 mm on NBRIP agar in the fungal isolates (Table 5). The halo size of 2.0 to 5.0 mm on PVK agar and 5.0 to 13.0 mm on MPVK agar was reported in bacterial isolates [7]. The halos were distinctively bright around fungal than bacteria.…”

Section: P Solubiliser Population In Different Mediamentioning

confidence: 94%

“…PSM were isolated from soil samples (Table 1) by serial dilution using spread plating on PVK [6], MPVK [7] and NBRIP [8] supplemented with tricalcium phosphate as insoluble inorganic phosphate source. The population of PSM was determined on the bases of ratio of number of phosphate solubilisers to total number of colonies and expressed as percentage.…”

Section: Methodsmentioning

confidence: 99%

“…The organism not forming halo on one media can produce clear zone on other media and can further be tested for P solubilisation in broth. Various workers have used different media for above studies [6,7,8]. It was of interest to screen the available media to show the halos clearly for easy identifi cation of the PSM.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterisation of phosphate solubilising microorganisms from the cold desert habitat of Salix alba Linn. in trans Himalayan region of Himachal Pradesh

2008

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Phosphate solubilising microorganisms (PSM) (bacteria and fungi) associated with Salix alba Linn. from Lahaul and Spiti valleys of Himachal Pradesh were isolated on Pikovskaya (PVK), modifi ed Pikovskaya (MPVK) and National Botanical Research Institute agar (NBRIP) media by spread plating. The viable colony count of P-solubilising bacteria (PSB) and fungi (PSF) was higher in rhizosphere than that of non-rhizosphere. The frequency of PSM was highest on MPVK followed by NBRIP and PVK agar. The maximum proportion of PSM out of total bacterial and fungal count was found in upper Keylong while the least in Rong Tong. The PSB frequently were Gram-positive, endosporeforming, motile rods and belonged to Bacillus sp. The PSF mainly belonged to Penicillium sp., Aspergillus fumigatus, A. niger, A. spp. and non-sporulating sterile. Amongst the isolates with high effi ciency for tricalcium phosphate (TCP) solubilisation, seven bacterial and seven fungal isolates dissolved higher amount of P from North Carolina rock phosphate (NCRP) than Mussoorie rock phosphate (MRP) and Udaipur rock phosphate (URP). However, the organisms solubilised higher-P in NBRIP broth than PVK broth. SBC5 (Bacillus sp.) and SBC7 (Bacillus sp.) bacterial isolates exhibited maximun P solubilisation (40 and 33 μg ml -1 respectively) whereas FC28 (Penicillium sp.) isolate (52.3 μg ml -1 ) amongst fungi while solubilising URP. The amount of P solubilised was positively correlated with the decrease in pH of medium. SBC5 (Bacillus sp.), SBC7 (Bacillus sp.) and SBC4 (Micrococcus) decreased the pH of medium from 6.8 to 6.08 while FC28 (Penicillium sp.) and FC39 (Penicillium sp.) isolates of fungi recorded maximum decrease in pH of medium from 6.8 to 5.96 in NBRIP broth.

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Section: P Solubiliser Population In Different Mediamentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

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Isolation and characterisation of phosphate solubilising microorganisms from the cold desert habitat of Salix alba Linn. in trans Himalayan region of Himachal Pradesh

2008

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“…Within this context, several studies have investigated the capacity of microorganisms for P solubilization and mineralization in different culture media with varying P sources (Pikovskaya 1948, Gupta et al 1994, Nautiyal 1999. The study of lowsolubility inorganic P sources, such as tricalcium phosphate (Ca 3 PO 4 ), aluminum phosphate (Al 3 PO 4 ) and iron phosphate (Fe 3 PO 4 ), and various organic P sources contributes to our understanding of the aforementioned mechanisms and to the selection of strains with high performance in both in vitro and field conditions (Bashan et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Phosphate solubilization by endophytic bacteria isolated from banana trees

Matos

Gomes

Nietsche

et al. 2017

An. Acad. Bras. Ciênc.

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Forty isolates of endophytic bacteria isolated from banana tree roots were assessed as to their capacity to solubilize phosphate in a solid culture medium supplemented with different inorganic and one organic source of phosphorus. The amount of phosphorus (P) in each liquid medium was quantified, and an indirect assessment of acid phosphatase activity was performed. All assays had a fully randomized design, with three repetitions. Approximately 67.5% of the 40 isolates assessed in solid medium solubilized phosphorus from tricalcium phosphate and 7.5% of the isolates solubilized phosphorus from soy lecithin; no isolates exhibited P solubilization capacity in medium supplemented with iron phosphate . Acid phosphatase activity was detected in 65% of the isolates; Aneurinibacillus sp. and Lysinibacillus sp. isolates presented with the best solubilization indexes. All of the assessed isolates exhibited a capacity to reduce the potential of hydrogen in liquid medium supplemented with tricalcium phosphate. Isolate EB. 78 (Bacillus sp.) exhibited P solubilization capacity in solid media when Ca 3 (PO 4 ) 2 and soy lecithin were used as P sources; this isolate significantly reduced the pH of the liquid medium and exhibited acid phosphatase activity. The results of the present study highlight isolates that exhibit variations in their capacity to solubilize P. These isolates should be used in future tests to assess their field performance.

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“…An important function of soil microorganisms is to supply nutrients for soil fertility, plant growth, and optimum crop production. Numerous efforts have been made to develop of specific media to isolate microorganisms capable of dissolving fixed or minimally-soluble forms of P (Pikovskaya 1948;Gupta et al 1994;Nautiyal 1999). Many of these efforts successfully resulted in the isolation of several microbial species capable of dissolving sparingly soluble P forms such as Acinetobacter sp., Klebsiella sp., Enterobacter sp., Microbacterium sp., and Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Pantoea Sp. Strain MK1D, and Erwinia Sp. Strain MK2Y as Tricalcium Phosphate Dissolving Bacteria Isolated from Calcareous Soil

K¹,

Il²

2017

Alexandria Science Exchange Journal: An International Quarterly

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This study aimed to isolate and characterize bacterial strains capable of solubilizing tricalcium phosphate (TCP) as the most commonly and insoluble phosphate in soils. Normal and molecular approaches were used to isolate and characterize phosphate dissolving bacteria (PDB) adapted to arid and semi-arid climate conditions. In this study, two strains were successfully isolated and taxonomically classified as Pantoea sp. strain MK1D, and Erwinia sp. strain MK2Y based on sequences of their 16S rRNA gene and were given GenBank accession numbers KU358676 and KU358677, respectively. This was confirmed by the formation of large halo zones on both Pikovskaya (PVK) and National Botanical Research Institute's Phosphate (NBRIP) culture media. The significant bacterial growth on liquid media was associated with the reduction of pH from 7.0 to 2.0 which lead to 83% solubilization of added TCP of 0.75 g/L. This study confirmed that both PVK and NBRIP media were reliable and comparable for the isolation of PDB and measuring their efficiency. Further studies are needed to confirm the effectiveness of these strains under pot and field conditions and their potential for commercial biofertilizers production.

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A modified plate assay for screening phosphate solubilizing microorganisms.

Cited by 199 publications

References 10 publications

Isolation and characterisation of phosphate solubilising microorganisms from the cold desert habitat of Salix alba Linn. in trans Himalayan region of Himachal Pradesh

Isolation and characterisation of phosphate solubilising microorganisms from the cold desert habitat of Salix alba Linn. in trans Himalayan region of Himachal Pradesh

Phosphate solubilization by endophytic bacteria isolated from banana trees

Characterization of Pantoea Sp. Strain MK1D, and Erwinia Sp. Strain MK2Y as Tricalcium Phosphate Dissolving Bacteria Isolated from Calcareous Soil

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