Phosphate solubilization by endophytic bacteria isolated from banana trees

Abstract

Forty isolates of endophytic bacteria isolated from banana tree roots were assessed as to their capacity to solubilize phosphate in a solid culture medium supplemented with different inorganic and one organic source of phosphorus. The amount of phosphorus (P) in each liquid medium was quantified, and an indirect assessment of acid phosphatase activity was performed. All assays had a fully randomized design, with three repetitions. Approximately 67.5% of the 40 isolates assessed in solid medium solubilized phosphorus from tricalcium phosphate and 7.5% of the isolates solubilized phosphorus from soy lecithin; no isolates exhibited P solubilization capacity in medium supplemented with iron phosphate . Acid phosphatase activity was detected in 65% of the isolates; Aneurinibacillus sp. and Lysinibacillus sp. isolates presented with the best solubilization indexes. All of the assessed isolates exhibited a capacity to reduce the potential of hydrogen in liquid medium supplemented with tricalcium phosphate. Isolate EB. 78 (Bacillus sp.) exhibited P solubilization capacity in solid media when Ca 3 (PO 4 ) 2 and soy lecithin were used as P sources; this isolate significantly reduced the pH of the liquid medium and exhibited acid phosphatase activity. The results of the present study highlight isolates that exhibit variations in their capacity to solubilize P. These isolates should be used in future tests to assess their field performance.

Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials

“…TNC-1, the highest amount of soluble P was associated with a decrease in the pH to 4.93. These data indicate that the solubilization process of P is consistent with the bacterial excretion of substances that acidified the culture broth [61]. Several reports indicate that organic acid (OA) and siderophores production are among the mechanisms underlying inorganic P solubilization [62,63].…”

Endophytic Bacillus megaterium BM18-2 mutated for cadmium accumulation and improving plant growth in Hybrid Pennisetum

“…Additionally, Phosphorus is essential element for plants growth and development, but its present in agriculture soils in low amount [44,45]. Endophytes are known to improve plant growth by the phosphate solubilization [46,47]. In the study conducted by Ullah et al [43] all tested endophytic bacterial strains were found to have the potential to solubilize phosphorous.…”

Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo

“…There was also a greater potential for solubilization of AP by endophytic bacteria compared to rhizosphere bacteria ( Figure 1A, p < 0.05). This effect of the origin site of bacteria of maize microbiome on P solubilization still needs to be further investigated, since numerous studies report this potential for bacteria isolated both from internal maize tissues (Matos et al, 2017;de Sousa et al, 2020) and the plant rhizosphere (Gomes et al, 2014;Li et al, 2017). It was expected that this greater potential was more related to rhizospheric bacteria that live in soil containing P insoluble forms.…”