A modified enzymic browning assay for potato glandular trichomes

Avé, D. A.; Eannetta, Nancy T.; Tingey, Ward M.

doi:10.1007/bf03044055

Cited by 13 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genotypes carrying both attributes are highly desirable in potato breeding for insect resistance because plants bearing both types of trichomes have been reported to be more resistant to green peach aphid than those with type A trichomes alone (Gibson, 1979). It has been shown that several cultivated potatoes have A glandular trichomes and that potato plants are resistant to insects only if their trichome biochemical component resembles that of S. tarijense, S. polyadenium, or S. berthaultii (Avé et al, 1986); therefore, assessing PPO activity should be considered an important component in the development of populations with resistance to insects, since it indicates the amount of oxidases present in glandular trichomes.…”

Section: Resultsmentioning

confidence: 99%

“…The density of A and B trichomes was evaluated using a randomized complete-block design with three replications, fifteen families, ten plants per family, two leaves per plant, and two spots per leaf. PPO activity was assessed following the procedures proposed by Avé et al (1986). Analyses of variance were done using PROC GLM (SAS Institute, 1985) and variance components were estimated using PROC VARCOMP (SAS Institute, 1985).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Genetics and Incorporation of Glandular Trichomes and Polyphenol Oxidase Activity into an Advanced Solanum phureja-S. stenotomum Diploid Potato Population

Vallejo¹,

Collins²,

Schiavone³

1994

jashs

View full text Add to dashboard Cite

Preliminary data on the genetics of glandular trichomes and the feasibility of incorporating A and B glandular trichomes and polyphenol oxidase (PPO) activity from Solanum berthaultii Hawkes (Ber) into an advanced S. phureja Juz. et Buk.-S. stenotomum Juz. (Phu-Stn) diploid potato population are presented. A random sample of four Phu-Stn clones was intercrossed with a sample of three Ber progenies (pollen bulk per progeny) segregating for high density of A and B glandular trichomes. Nine F1 families [(Phu-Stn) × Ber] were evaluated over two experiments in the greenhouse for A and B trichome density (5 mm2 of abaxial surface) and PPO activity 45 days after planting. A completely randomized design in an experimental hierarchical classification arrangement was used to quantify the genetic variability and to estimate broad-sense heritabilities (defined on an individual basis) for the attributes under study. To characterize the inheritance of these attributes further and to estimate narrow-sense heritabilities, fifteen backcross (BC) families were evaluated in a randomized complete-block design with three replications and analyzed by half-sib family analysis. Phenotypic and genotypic correlations between these attributes were also estimated. In the F1 families, broad-sense heritabilities were 0.59 and 0.41 for density of A and B trichomes, respectively. In the BC population, narrow-sense heritabilities were 0.15 and 0.08 for A trichome density and PPO activity, respectively; expression of B trichomes was almost absent in this generation. Genotype × environment interaction was significant for density of A trichomes and highly significant for density of B trichomes in F1 families. There was some level of positive association between density of A and B trichomes and PPO activity. Additive genetic variance of these attributes was very low in this study; therefore, selection schemes based on family selection and progeny testing would be an appropriate breeding strategy for improving these traits.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genetics and Incorporation of Glandular Trichomes and Polyphenol Oxidase Activity into an Advanced Solanum phureja-S. stenotomum Diploid Potato Population

Vallejo¹,

Collins²,

Schiavone³

1994

jashs

View full text Add to dashboard Cite

show abstract

Insect-resistant plants with improved horticultural traits from interspecific potato hybrids grown in vitro

Lentini

Earle

Plaisted

1990

Theoret. Appl. Genetics

View full text Add to dashboard Cite

Plants were regenerated from petiole calli of interspecific hybrids of Solanum tuberosum x S. berthaultii, an insect-resistant wild species. Callus culture was used to generate genetic changes to overcome the restricted recombination between the two genomes. Two plants out of 58 (3.5%) from calli of hybrid J114-1 showed stable and heritable differences from the hybrid over two cycles of evaluations in the field. Replicated trials were conducted in 1987 and 1988, using two populations of plants propagated by nodal cuttings from the original regenerates maintained in vitro. One regenerate showed insect resistance and increased marketable yield (approximately two fold) in the field. The other had higher levels of phenolic exudate in one of the two types of foliar trichomes associated with the insect resistance mechanism. Some desirable changes were discernible only in sexual progeny of regenerates, not in the regenerates themselves. In a backcross to S. tuberosum, 7 of 14 (50%) regenerates from hybrid F743-4 showed more progeny (up to 15-fold) with improved trichome traits and horticultural characteristics than the original hybrid. The variations were not associated with changes in ploidy. Fifteen plants obtained from these crosses are currently being incorporated into breeding lines. These results suggest that a period of callus culture followed by plant regeneration may aid in the introgression of desirable traits from wild species into crop plants.

show abstract

QTL analysis of trichome-mediated insect resistance in potato

Bonierbale

Plaisted

Pineda

et al. 1994

Theoret. Appl. Genetics

158

View full text Add to dashboard Cite

Genetic mapping of several components of a complex type of insect resistance has been undertaken as a means toward more efficient use of the valuable characteristics of a wild relative of potato. RFLP maps constructed on interspecific diploid progenies of Solanum tuberosum × S. berthaultii were used in conjunction with morphological, biochemical and biological phenotyping to identify quantitative trait loci (QTLs) contributing to trichome-mediated insect resistance. By superimposing QTL data for a wide range of phenotypes including biochemical assays, correlative and direct screens for insect resistance, and adaptation to the target environment on the genetic maps, we have addressed the organization, action and interaction of genes controlling the resistance mechanism. The outcome contributes to an understanding of the association between component traits and between desirable and undesirable features of the donor species generated in an applied breeding program. Research is proceeding toward the development of selectable markers for the introgression and transfer of this resistance among potato gene pools.

show abstract

A modified enzymic browning assay for potato glandular trichomes

Cited by 13 publications

References 8 publications

Genetics and Incorporation of Glandular Trichomes and Polyphenol Oxidase Activity into an Advanced Solanum phureja-S. stenotomum Diploid Potato Population

Genetics and Incorporation of Glandular Trichomes and Polyphenol Oxidase Activity into an Advanced Solanum phureja-S. stenotomum Diploid Potato Population

Insect-resistant plants with improved horticultural traits from interspecific potato hybrids grown in vitro

QTL analysis of trichome-mediated insect resistance in potato

Contact Info

Product

Resources

About