QTL analysis of trichome-mediated insect resistance in potato

Abstract

Genetic mapping of several components of a complex type of insect resistance has been undertaken as a means toward more efficient use of the valuable characteristics of a wild relative of potato. RFLP maps constructed on interspecific diploid progenies of Solanum tuberosum × S. berthaultii were used in conjunction with morphological, biochemical and biological phenotyping to identify quantitative trait loci (QTLs) contributing to trichome-mediated insect resistance. By superimposing QTL data for a wide range of phenotypes including biochemical assays, correlative and direct screens for insect resistance, and adaptation to the target environment on the genetic maps, we have addressed the organization, action and interaction of genes controlling the resistance mechanism. The outcome contributes to an understanding of the association between component traits and between desirable and undesirable features of the donor species generated in an applied breeding program. Research is proceeding toward the development of selectable markers for the introgression and transfer of this resistance among potato gene pools.

QTL analysis of morphological traits in eggplant and implications for conservation of gene function during evolution of solanaceous species

“…A cluster of eggplant hairiness loci on linkage group 10 also has potential counterparts in both tomato and potato. The map position of this cluster of apex, leaf and stem hairiness QTL corresponds to that of a type B trichome density QTL on chromosome 5 in potato (Bonierbale et al 1994) and a type IV trichome density QTL on the same chromosome in tomato (Maliepaard et al 1995). The densities of both type B and type IV trichomes have been associated with insect resistance in their respective crops.…”

“…A previous study had examined the genetic control of domestication traits in the same population (Doganlar et al 2002b). The use of interspecific F 2 and advanced backcross populations has proven to be an effective and efficient way of mapping agronomic and horticultural traits in eggplant's relatives: tomato, potato and pepper (e.g., Bonierbale et al 1994;Tanksley et al 1996;Ben Chaim et al 2001). In addition to providing adequate phenotypic and DNA polymorphism which may be limiting in intraspecific eggplant populations (Nunome et al 2001), interspecific populations can supply the starting material for the introgression of valuable traits from wild species into eggplant cultivars.…”

QTL hotspots in eggplant (Solanum melongena) detected with a high resolution map and CIM analysis

“…On chromosome 10, sh10.1 and lh10.1 mapped 25 cM apart suggesting the Jong et al (2004) existence of two hairiness genes in fairly close proximity on this chromosome. Trichome QTL in tomato (TriIV-1, type IV trichome production; Maliepaard et al 1995) and potato (type B trichome density; Bonierbale et al 1994) are possible orthologs of sh10.1 and lh10.1. Additional QTL influencing leaf and apex hairiness mapped in independent locations on chromosomes 2 (ah2.1), 3 (ah3.2, ah3.3), 7 (ah7.1), and 9 (lh9.1) indicating that additional unrelated loci of slightly lesser effect are controlling these traits.…”

Evidence from Polygene Mapping for a Causal Relationship between Potato Tuber Dormancy and Abscisic Acid Content

“…We have examined two populations obtained by backcrossing to the respective parent species a hybrid of haploid potato by a wild species (Solanum berthaultii). RFLP mapping of these populations has been carried out (Bonierbale et al, 1994). We have used the mapping information to identify 11 QTLs associated with the ability to tuberize under long days (Van den Berg et al, 1996a) and 9 QTLs for tuber dormancy (Van den Berg et al, 199613).…”