A modification of Van Kampen-Zijlstra's reagent for the hemiglobincyanide method

Matsubara, Takahiro; Hiroshi, Okuzono; Senba, Umeko

doi:10.1016/0009-8981(79)90259-6

Cited by 23 publications

(2 citation statements)

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“…where D i is the result of the integration of the signal over the time window T i . Moreover, it has already been demonstrated that for a time window much longer than the lifetime, T 3 τ, the value of the mean lifetime of an n th -order exponential can be obtained assuming a mono-exponential decay with notional lifetime τ [20]. Thus, the integration of the decay over the time window T 3 results in…”

Section: Room-temperature Phosphorescence Hb Determinationmentioning

confidence: 99%

“…Different procedures have been proposed for Hb determination in clinical diagnosis such as optical [16,17], electrochemical [18], or liquid chromatography [19]. A widely used method is the spectrophotometric procedure based on the Van Kampen-Zijlstra reagent, although it uses the toxic alkaline cyanide as a reagent among other disadvantages [20]. Several carbon dot (CD) fluorescent sensors for Hb determination have been published in recent years [21,22].…”

Section: Introductionmentioning

confidence: 99%

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Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots

et al. 2020

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A portable reconfigurable platform for hemoglobin determination based on inner filter quenching of room-temperature phosphorescent carbon dots (CDs) in the presence of H2O2 is described. The electronic setup consists of a light-emitting diode (LED) as the carbon dot optical exciter and a photodiode as a light-to-current converter integrated in the same instrument. The reconfigurable feature provides adaptability to use the platform as an analytical probe for CDs coming from different batches with some variations in luminescence characteristics. The variables of the reaction were optimized, such as pH, concentration of reagents, and response time; as well as the variables of the portable device, such as LED voltage, photodiode sensitivity, and adjustment of the measuring range by a reconfigurable electronic system. The portable device allowed the determination of hemoglobin with good sensitivity, with a detection limit of 6.2 nM and range up to 125 nM.

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Section: Room-temperature Phosphorescence Hb Determinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots

et al. 2020

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Histopathological changes of rat brain after direct injection of Hb‐vesicles (artificial oxygen carriers) and neurological impact in an intracerebral hemorrhage model

Sakai

Okamoto

Ikeda

et al. 2008

J Biomedical Materials Res

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For use as artificial oxygen carriers during transfusion, the safety and efficacy of Hb-vesicles (HbV, 250 nm), have been investigated extensively. Nevertheless, their neurotoxicity remains unknown. We explored potential adverse effects of HbV in the brain using a rat intracerebral hemorrhage model. Male Wistar rats were anesthetized with sevoflurane and fixed on a stereotaxic frame. Then HbV or homologous RBC suspension ([Hb] = 8.6 g/dL, 20 microL) was injected into the right caudate nucleus. All animals survived, gained weight, and maintained their well-being until the time of sacrifice; except during the first few days after surgery. However, both groups showed slight weakness in hind leg retraction, occasional ataxia/gait, and piloerection. Neutrophils accumulated at the onset of injury in perihematomal tissues in both groups at 1st day, but had disappeared by 3 days. Infiltration of small HbV in the perihematomal tissue was prominent at 1st day; phagocytized HbV were detected in macrophages. Hemeoxygenase-1 and hemosiderin deposition appeared after 3 days, reflecting the degradation of both HbV and RBC. The HbVs were detectable even after 28 days in the HbV group, but no residual RBCs were detected in the RBC group. Both groups showed proliferation of astrocytes, named gliosis, for tissue reconstruction after 3 days. This study revealed no notable differences in adverse effects between the intra-cerebral injection of HbV and the RBC control on behavioral functions and brain tissue responses.

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A new reagent for the rapid determination of total hemoglobin as hemiglobincyanide in blood containing carboxyhemoglobin

Sato

Katsumata

Aoki

et al. 1983

Biochemical Medicine

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A modification of Van Kampen-Zijlstra's reagent for the hemiglobincyanide method

Cited by 23 publications

References 1 publication

Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots

Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots

Histopathological changes of rat brain after direct injection of Hb‐vesicles (artificial oxygen carriers) and neurological impact in an intracerebral hemorrhage model

A new reagent for the rapid determination of total hemoglobin as hemiglobincyanide in blood containing carboxyhemoglobin

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