A new reagent for the rapid determination of total hemoglobin as hemiglobincyanide in blood containing carboxyhemoglobin

Sato, Keizo; Katsumata, Yoshinao; Aoki, Minoru; Сузукі, Осаму; Kido, Akira; Oya, Masakazu; Yada, S

doi:10.1016/0006-2944(83)90010-8

Cited by 11 publications

(4 citation statements)

References 12 publications

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“…The following values were used for to quantify porphyrins, their derivatives, and hemoglobin forms: hemin, 350 ) 90 mM (21); and CoHbO 2 , 422 ) 120 mM -1 cm -1 in 0.1 M sodium phosphate (NaPO 4 ) (22). Hb + Met, HbO 2 , and HbCO were all converted to Hb + CN derivatives and quantified using an 540 of 44.0 mM -1 cm -1 and a molecular weight for Hb of 64 458 (23).…”

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confidence: 99%

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Structural Requirements for Hemoglobin To Induce Fibronectin Receptor Expression in Candida albicans

2000

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Hemoglobin (Hb) is a host factor that induces expression of a promiscuous receptor on Candida albicans for fibronectin (FN) and several other extracellular matrix proteins. FN receptor expression was induced by ferric (Hb(+)Met and Hb(+)CN), ferrous (HbCO and HbO(2)), and cobalt-protoporphyrin derivatives of Hb, whereas globin was inactive. The Hb derivatives all exhibited saturable, dose-dependent kinetics of FN receptor induction, suggesting that Hb may be acting as a receptor ligand. Soluble Hb bound saturably to a low-affinity binding site [K(d) = (1.1+/-0.2) x 10(-6) M] on C. albicans blastospores. However, uptake of (55)FeHb revealed that heme or iron transport into the cell is not required for induction, since internalization of (55)Fe from Hb did not occur until after induction of FN binding. The serum Hb-binding protein, haptoglobin, specifically abrogated this response, indicating that protein structure rather than the heme ligand or iron is necessary for induction of this signaling pathway. C. albicans also adhered to immobilized Hb, which was sufficient to induce FN receptor expression, and to Hb polymers that formed in defined Hb liquid media in the presence of cells. Formation of Hb polymers in solution required metabolic energy, since the aggregation process was halted with azide addition. Collectively, these data demonstrate that C. albicans recognizes polymerized Hb through multivalent low-affinity interactions, and this may be a host environmental cue that triggers extracellular matrix receptor expression at a septic site.

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confidence: 99%

“…Hb + CN was prepared from HbO 2 by oxidation with potassium ferricyanide in the presence of potassium cyanide (23). The ferrocyanide resulting from the oxidation was removed by gel chromatography using Sephadex G-25 (PD-10, Amersham Pharmacia Biotech).…”

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confidence: 99%

Structural Requirements for Hemoglobin To Induce Fibronectin Receptor Expression in Candida albicans

2000

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“…Correction for the hematocrit bias in dried blood spot was detailed before [ 35 ] and the hematocrit levels can be easily obtain from the hemoglobin levels using formula hematocrit (HCT)=2.941*hemoglobin concentration g/dl [ 36 ]. Hemoglobin is routinely measured using the Darbkin reagent and UV detection, using only 10 μl of frozen WB [ 37 ]. Therefore, pairing WB lipidomics with hemoglobin measurements will allow a simple calculation of the sphingolipids levels in erythrocytes, as was shown before for other metabolites from RBC [ 38 ].…”

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Analytical Considerations for Reducing the Matrix Effect for the Sphingolipidome Quantification in Whole Blood

Wang

Mesaros

2021

Bioanalysis

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Aim: Plasma and serum are widely used blood-derived biofluids for metabolomics and lipidomics assays, but analytes that are present in high concentrations in blood cells cannot be evaluated in those samples and isolating serum or plasma could introduce additional variability in the data. Materials & methods: In this study, we provide a comprehensive method for quantification of the whole blood (WB) sphingolipidome, combining a single-phase extraction method with LC–high-resolution mass spectrometry. Results: We were able to quantify more than 150 sphingolipids, and when compared with paired plasma, WB contained higher concentration of most sphingolipids and individual variations were lower. These findings suggest that WB could be a better alternative to plasma, and potentially guide the evaluation of the sphingolipidome for biomarker discovery.

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“…Correction for the hematocrit bias in dried blood spot was detailed before [28] and the hematocrit levels can be easily obtain from the hemoglobin levels using formula HCT=2.941*hemoglobin conc g/dL [29]. Hemoglobin can be easily measured using the Darbkin reagent and UV detection, using only 10 µL of frozen whole blood [30]. Using the hemoglobin measurements would be easy to calculate the sphingolipids levels in erythrocytes as was shown before for folates [31].…”

Section: Whole Blood Has a Richer Abundance Of Sphingolipids Compared With Paired Plasmamentioning

confidence: 99%

Sphingolipidome Quantification by Liquid Chromatography- High Resolution Mass Spectrometry: Whole Blood vs. Plasma

Wang¹,

Xu²,

Mesaros³

2021

Preprint

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Plasma and serum are the most widely used blood-derived biofluids for metabolomics and lipidomics assays, but the isolation of these products from blood may introduce additional bias as indicated by the fact that many analytes that are present at high concentrations in blood cells cannot be measured and evaluated in those samples. Of particular concern, variable hemolysis during the pre-processing of blood products could compromise accurate and reproducible quantification. Compared with plasma or serum, whole blood may be a better alternative due to simplicity of processing. In this study, we provide a comprehensive method for quantification of the whole blood sphingolipidome and the concentrations were compared with those from plasma. Combining a single-phase extraction method with liquid-chromatography high resolution mass spectrometry (R=120, 000), assisted by alkaline hydrolysis, we were able to identify and simultaneously quantify more than 150 sphingolipids. Furthermore, most of sphingolipids remained stable after a freeze/thaw cycle. Whole blood contained a higher concentration of most sphingolipids than corresponding plasma. Moreover, individual variations in the levels of sphingolipids were lower for whole blood than plasma. These findings demonstrate that whole blood could be a better alternative to plasma, and potentially guide the evaluation of sphinglipidome for biomarker discovery.

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A new reagent for the rapid determination of total hemoglobin as hemiglobincyanide in blood containing carboxyhemoglobin

Cited by 11 publications

References 12 publications

Structural Requirements for Hemoglobin To Induce Fibronectin Receptor Expression in Candida albicans

Structural Requirements for Hemoglobin To Induce Fibronectin Receptor Expression in Candida albicans

Analytical Considerations for Reducing the Matrix Effect for the Sphingolipidome Quantification in Whole Blood

Sphingolipidome Quantification by Liquid Chromatography- High Resolution Mass Spectrometry: Whole Blood vs. Plasma

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