A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection

Abstract

A mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MPVQ) for biological zinc ions detection was developed based on quinolone platform. Mito-MPVQ showed large red shifts (68nm) and selective ratiometric signal upon Zn2+ binding. The ratio of emission intensity (I488 nm/I420 nm) increases dramatically from 0.45 to 3.79 (ca. 8-fold). NMR titration and theoretical calculation confirmed the binding of Mito-MPVQ and Zn2+. Mito-MPVQ also exhibited large two-photon absorption cross sections (150GM) at nearly 720 nm and insensitivity to pH within the biologically relevant pH range. Cell imaging indicated that Mito-MPVQ could efficiently located in mitochondria and monitor mitochondrial Zn2+ under two-photon excitation with low cytotoxicity.

Anti-tumour activity of zinc ionophore pyrithione in human ovarian cancer cells through inhibition of proliferation and migration and promotion of lysosome-mitochondrial apoptosis

“…However, there is no consistent conclusion about the subcellular localization of supplementary Zinc in the cells. Some studies suggested that Zinc targeted in mitochondria [43,44], while other researches advocated that Zinc could trigger apoptosis by accumulating Zinc into the lysosome [45,46]. In our study, in accordance, we observed that ZPT transported Zinc mostly into lysosomes, accumulated Zinc could cause the lysosomal membrane permeabilization (LMP) and consequent cathepsin D distribution.…”

A biocompatible two-photon absorbing fluorescent mitochondrial probe for deepin vivobioimaging

“…9–11 For example, a mitochondrial probe QBMP synthesized with a favorable 2PA cross-section (70.5 GM) achieved a tissue penetration depth of 225 μm in in vivo bioimaging. 9–11 However, these mitochondrial probes still suffer from low two-photon absorption properties 11–13 or poor biocompatibility, 14,15 restricting their application in live animal bioimaging. This study developed a water-soluble probe with low cytotoxicity and a relatively high 2PA cross-section of ca.…”

Bicolour fluorescent molecular sensors for cations: design and experimental validation

“…Representative examples are shown in Figure 9. [70][71][72][73] MPVQ-series, initially developed at Meng's group, 73 consisted of BPA-appended quinoline as fluorescent skeleton with a conjugated donor on the 6-position, whereas chromis-1 contains a 2-arylthiazol as a push-pull fluorophore. In all cases, a red-shifted fluorescence was observed in presence of Zn 2+ , which was accompanied by an increased TPA cross-section.…”