A microtest for the quantitation of rabies virus neutralizing antibodies

Zalan, E.; Wilson, C.; Pukitis, Dzidra

doi:10.1016/s0092-1157(79)80024-4

Cited by 112 publications

(47 citation statements)

References 5 publications

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“…Rabies virus neutralizing antibody titers (in IU/ml) were determined according to the rapid focus fluorescent inhibition test (RFFIT) adapted on 96 microplates. 10,11 Statistical analysis. To study the kinetics of rabies antibody levels in the dogs that were sampled at various intervals, we verified that the individual antibody titers expressed by the decimal logarithm of the number of IU/ml was a normal variable.…”

Section: Methodsmentioning

confidence: 99%

Rabies mass vaccination campaigns in Tunisia: are vaccinated dogs correctly immunized?

Seghaier¹,

Cliquet²,

Hammami³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. Among the 301 dogs vaccinated against rabies in a suburban area of Tunis, 165 were sero-surveyed for 13 months. One month after vaccination, 65% of the dogs less than 1 year of age and 76-84% of the older dogs had significant antibody titers. One month after annual revaccination, these percentages ranged between 92% and 100%. Puppies (less than 3 months old) responded to vaccination with no significant interference by passive maternal immunity. Based on these percentages, a 93% rate of protection may be expected for vaccinated dogs. This study confirms that all dogs (even those less than 3 months of age) must be vaccinated during mass campaigns. The expected protection conferred by locally produced potent vaccines reaches 79-99% based on the age of the dogs. The alleged relative inability of local dogs to respond to vaccination cannot explain the partial success of rabies control in Tunisia.

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Section: Methodsmentioning

confidence: 99%

Rabies mass vaccination campaigns in Tunisia: are vaccinated dogs correctly immunized?

Seghaier¹,

Cliquet²,

Hammami³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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“…This test was developed based on the "Rapid Fluorescent Focus Inhibition Test" (RFFIT) [17] and "Fluorescent Inhibition Microtest" (FIMT) [18]. CVS11 virus and the 2 nd International Standard for anti-rabies serum were used as controls.…”

Section: Titration Of Anti-rabiesmentioning

confidence: 99%

Effects of primovaccination and booster vaccination on serum cortisol and humoral immune response in cattle

Reis¹,

Pardo²,

Frazatti-Gallina³

et al. 2013

ABB

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The objective of this study was to evaluate the effect of one or two doses of the anti-rabies vaccination on the serum concentration of cortisol and the humoral immune response in cattle as well as the correlation between serum cortisol concentrations and the titers of rabies-neutralizing antibodies. Nelore cattle were randomly assigned to one of three groups, which were vaccinated with one dose of rabies vaccine (group GVSR, N = 15), two doses of rabies vaccine (group GVR, N = 15) or were not vaccinated (group Gc, N = 15). A commercial liquid inactivated rabies vaccine was used. The stressors imposed on the cattle were vaccination, corral handling and the presence of people. Blood samples were collected on days 0, 30 and 60 post-vaccination. Serum cortisol concentrations were determined using a solid-phase radioimmunoassay, and rabies antibody titers were determined using a serum neutralization test with BHK 21 cells (RFFIT). Both serum cortisol concentrations and antibody titers increased after the second (booster) vaccination (P < 0.05). In all the groups, the serum cortisol concentrations increased after the cattle were handled in the corral (P < 0.05). No correlation was observed between the serum cortisol concentrations and the antibody titers with any treatment or on any observation day. In conclusion, booster vaccination is indispensable for primovaccinated cattle in achieving high and protective levels of rabies antibodies. Although booster vaccination and frequent cattle handling in corrals are stressors, the response is not strong enough to cause immunosuppression in cattle.

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“…Antibody testing was performed using the rapid fluorescent focus inhibition test (RFFIT) in 96-well microplates according to the technique of Zalan et al (10).…”

mentioning

confidence: 99%

Promising Rabies Vaccine for Postexposure Prophylaxis in Developing Countries, a Purified Vero Cell Vaccine Produced in China

Wang

Zhang

Song

et al. 2010

Clin Vaccine Immunol

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A microtest for the quantitation of rabies virus neutralizing antibodies

Cited by 112 publications

References 5 publications

Rabies mass vaccination campaigns in Tunisia: are vaccinated dogs correctly immunized?

Rabies mass vaccination campaigns in Tunisia: are vaccinated dogs correctly immunized?

Effects of primovaccination and booster vaccination on serum cortisol and humoral immune response in cattle

Promising Rabies Vaccine for Postexposure Prophylaxis in Developing Countries, a Purified Vero Cell Vaccine Produced in China

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