Respirometry is the measurement of the biological oxygen consumption rate under well-defined conditions. This technique is generally employed to determine the kinetic and stoechiometric parameters of biological processes, such as: the oxygen transfer coefficient K L a, the endogenous oxygen uptake rate OUR end , exogenous oxygen uptake rate OUR exo , the total oxygen uptake rate OUR t ; the substrate affinity constant (K S ), the maximum growth rate (μ max ) and the yield (Y X/S ). According to the literature, there is a large variability in the estimation of these kinetic and stoechiometric parameters, attributed to three key factors: the culture history, the parameter identifiability and the initial substrate to biomass ratio (S 0 /X 0 ). K L a, depends on endogenous oxygen uptake rate OUR end , is a key constant in evaluating respirogramme-specific parameters. Generally, OUR end is assumed constant during dissolved oxygen variation. The effect of S 0 /X 0 ratio on kinetic and stoechiometric parameters has only been partially studied. The goal of this study was to assess respirometrically the effect of various S 0 /X 0 ratios (in the range of 0.01 to 0.23) on the kinetic and stoechiometric parameters of a heterotrophic activated sludge biomass (presence of ATU a nitrification inhibitor) and for both heterotrophicautotrophic activated sludge biomass.Key-words-Respirometry; S 0 /X 0 ratios; stoechiometric and kinetic parametres.
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