Kinetic and microbiological characterization of aerobic granules performing partial nitritation of a low-strength wastewater at 10 °C

Reino, Clara; Suárez-Ojeda, María Eugenia; Pérez, Julio; Carrera, Julián

doi:10.1016/j.watres.2016.05.059

Cited by 97 publications

(39 citation statements)

References 51 publications

(62 reference statements)

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“…The nitrifying aerobic granular sludge was highly enriched in AOB during the whole operation of the airlift reactor. When the samples were collected, the abundance of AOB was 92% ± 4% of the total population as quantified by fluorescence in situ hybridization (FISH) coupled to confocal laser scanning microscope as described in Isanta et al (2015) and Reino et al (2016) (Figure 2a). Regarding NOB, Nitrobacter spp.…”

Section: Nitrifying Aerobic Granular Sludge and The Dominant Microomentioning

confidence: 99%

“…The ammonium oxidation rate was extremely high at such low temperature (ca. 0.6 g·N·L −1 ·d −1 at 10°C) (Reino, Suárez‐Ojeda, Pérez, & Carrera, ). Interestingly, no EPS could be extracted from these granules when the protocols of ALE (Lin et al., ), Granulan (Seviour et al., ) and acidic soluble EPS (Pronk et al., ) extraction were followed.…”

Section: Introductionmentioning

confidence: 99%

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Glycosylated amyloid‐like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium‐oxidizing bacteria

et al. 2018

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A new type of structural extracellular polymers (EPS) was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and FTIR spectroscopy to characterize specific amino acids and protein secondary structure, and by SDS-PAGE with different stains to identify different glycoconjugates. Its intrinsic fluorescence was captured to visualize the location of the extracted EPS in the nitrifying granules, and its hydrogel-forming property was studied by rheometry. The extracted EPS is abundant with cross ß-sheet secondary structure, contains glycosylated proteins/polypeptides, and rich in tryptophan. It forms hydrogel with high mechanical strength. The extraction and discovery of glycosylated proteins and/or amyloids further shows that conventionally used extraction and characterization techniques are not adequate for the study of structural extracellular polymers in biofilms and/or granular sludge. Confirming amyloids secondary structure in such a complex sample is challengeable due to the possibility of amyloids glycosylation and self-assembly. A new definition of extracellular polymers components which includes glycosylated proteins and a better approach to studying them is required to stimulate biofilm research. K E Y W O R D Saerobic granular sludge, ammonium-oxidizing bacteria, amyloid, extracellular polymeric substances, glycoproteins

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Section: Nitrifying Aerobic Granular Sludge and The Dominant Microomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Glycosylated amyloid‐like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium‐oxidizing bacteria

et al. 2018

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“…Therefore, instead of aiming to demonstrate the long-term stability of the NOB 64 repression (as done recently at low temperatures in Isanta et al, 2015 andReino et al, 2016), 65 assessment of the short term effects of the residual ammonium concentration was specifically 66 targeted. Several techniques were used during the research.…”

Section: Introduction 27mentioning

confidence: 99%

Effects of the residual ammonium concentration on NOB repression during partial nitritation with granular sludge

et al. 2016

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Citation (APA)Poot Important noteTo cite this publication, please use the final published version (if applicable). Please check the document version above. CopyrightOther than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons. Takedown policyPlease contact us and provide details if you believe this document breaches copyrights. We will remove access to the work immediately and investigate your claim. This work is downloaded from Delft University of Technology. For technical reasons the number of authors shown on this cover page is limited to a maximum of 10.Effects of the residual ammonium concentration on NOB repression

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“…AOB and nitrite oxidizing bacteria (NOB), heterotrophic denitrifying bacteria and organics decomposing bacteria. 33 Similarly, Proteobacteria (59%) dominated an intermittent-aeration fluidized bed reactor-membrane bioreactor combo system for nutrient removal. 34 Actinobacteria was the second phylum in order, with the relative abundance of 20.56%, followed by Chloroflexi (16.67%) and Nitrospirae (9.06%).…”

Section: Nitrogen Conversion By Active Functional Microbial Communitymentioning

confidence: 99%

Enhanced nitrogen removal and in situ microbial community in a two‐step feed oxic/anoxic/oxic‐membrane bioreactor (O/A/O‐MBR) process

Zhijia

Wang

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND The carbon in raw sewage can be reclaimed to reduce nitrate for the advanced denitrification in municipal wastewater treatment plants. A novel two‐step feed oxic/anoxic/oxic‐membrane bioreactor (O/A/O‐MBR) process was proposed to improve carbon‐use efficiency. RESULTS The flow distribution ratio (FDR), aeration mode and hydraulic loading were optimized to minimize the effluent total nitrogen (TN) concentration. When the FDR was 1:1, the intermittent aeration mode was 4 min air‐on/6 min air‐off and the influent flow was 5 L h−1, the nitrate removal efficiency in the post‐denitrification tank rose to 87.0% and the effluent TN concentration decreased below 15.0 mg L−1. The intermittent aeration strategy in the post oxic‐membrane tank effectively promoted TN loss. The 15N‐DNA based stable isotope probing experiment illustrated that Proteobacteria (35.09%), Actinobacteria (20.56%) and Chloroflexi (16.67%) dominated the intermittently aerated biofilm, and Nitrospira (9.06%) was the major nitrite oxidizing bacteria rather than Nitrobacter, representing a dominant simultaneous nitrification and denitrification pathway for nitrogen removal. CONCLUSION The integration of autotrophic nitrifying bacteria and heterotrophic denitrifying bacteria enhances the terminal TN removal under intermittent aeration mode. The two‐step feed O/A/O‐MBR process has the potential to solve carbon limitation in sewage wastewater treatment. © 2018 Society of Chemical Industry

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Kinetic and microbiological characterization of aerobic granules performing partial nitritation of a low-strength wastewater at 10 °C

Cited by 97 publications

References 51 publications

Glycosylated amyloid‐like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium‐oxidizing bacteria

Glycosylated amyloid‐like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium‐oxidizing bacteria

Effects of the residual ammonium concentration on NOB repression during partial nitritation with granular sludge

Enhanced nitrogen removal and in situ microbial community in a two‐step feed oxic/anoxic/oxic‐membrane bioreactor (O/A/O‐MBR) process

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